ID A0A498R9F9_9FIRM Unreviewed; 161 AA.
AC A0A498R9F9;
DT 05-JUN-2019, integrated into UniProtKB/TrEMBL.
DT 05-JUN-2019, sequence version 1.
DT 24-JAN-2024, entry version 12.
DE RecName: Full=Cyanate hydratase {ECO:0000256|HAMAP-Rule:MF_00535};
DE Short=Cyanase {ECO:0000256|HAMAP-Rule:MF_00535};
DE EC=4.2.1.104 {ECO:0000256|HAMAP-Rule:MF_00535};
DE AltName: Full=Cyanate hydrolase {ECO:0000256|HAMAP-Rule:MF_00535};
DE AltName: Full=Cyanate lyase {ECO:0000256|HAMAP-Rule:MF_00535};
GN Name=cynS {ECO:0000256|HAMAP-Rule:MF_00535};
GN ORFNames=LUCI_2852 {ECO:0000313|EMBL:VBB07587.1};
OS Lucifera butyrica.
OC Bacteria; Bacillota; Negativicutes; Veillonellales; Veillonellaceae;
OC Lucifera.
OX NCBI_TaxID=1351585 {ECO:0000313|EMBL:VBB07587.1, ECO:0000313|Proteomes:UP000277811};
RN [1] {ECO:0000313|EMBL:VBB07587.1, ECO:0000313|Proteomes:UP000277811}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LUCI {ECO:0000313|EMBL:VBB07587.1};
RA Strepis N.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the reaction of cyanate with bicarbonate to produce
CC ammonia and carbon dioxide. {ECO:0000256|ARBA:ARBA00003561,
CC ECO:0000256|HAMAP-Rule:MF_00535}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+);
CC Xref=Rhea:RHEA:11120, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:28938, ChEBI:CHEBI:29195;
CC EC=4.2.1.104; Evidence={ECO:0000256|HAMAP-Rule:MF_00535};
CC -!- SIMILARITY: Belongs to the cyanase family. {ECO:0000256|HAMAP-
CC Rule:MF_00535}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; UPPP01000076; VBB07587.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A498R9F9; -.
DR OrthoDB; 9785870at2; -.
DR Proteomes; UP000277811; Unassembled WGS sequence.
DR GO; GO:0008824; F:cyanate hydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009439; P:cyanate metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd00559; Cyanase_C; 1.
DR CDD; cd00093; HTH_XRE; 1.
DR Gene3D; 3.30.1160.10; Cyanate lyase, C-terminal domain; 1.
DR Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1.
DR HAMAP; MF_00535; Cyanate_hydrat; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR008076; Cyanase.
DR InterPro; IPR003712; Cyanate_lyase_C.
DR InterPro; IPR036581; Cyanate_lyase_C_sf.
DR InterPro; IPR048564; CYNS_N.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR NCBIfam; TIGR00673; cynS; 1.
DR PANTHER; PTHR34186; CYANATE HYDRATASE; 1.
DR PANTHER; PTHR34186:SF2; CYANATE HYDRATASE; 1.
DR Pfam; PF02560; Cyanate_lyase; 1.
DR Pfam; PF21291; CYNS_N; 1.
DR PIRSF; PIRSF001263; Cyanate_hydratas; 1.
DR PRINTS; PR01693; CYANASE.
DR SMART; SM01116; Cyanate_lyase; 1.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF55234; Cyanase C-terminal domain; 1.
DR SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1.
DR PROSITE; PS50943; HTH_CROC1; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00535};
KW Reference proteome {ECO:0000313|Proteomes:UP000277811}.
FT DOMAIN 23..77
FT /note="HTH cro/C1-type"
FT /evidence="ECO:0000259|PROSITE:PS50943"
FT ACT_SITE 95
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00535"
FT ACT_SITE 98
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00535"
FT ACT_SITE 123
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00535"
SQ SEQUENCE 161 AA; 18275 MW; 50B8C055C384DEB0 CRC64;
MSATPVNNRD YLWDYREKAL AVLEKARLEK GLSYEEIAKK LEVSKVWLAS TFKGQQFVPA
DYTEKLATIL GLSIADVRVL QEHPYKGNVD PILYRLHEVF DTYGPAIKAI IHEKFGDGNG
IMSAIDFSVD VEKVEDPKGD RVVITFNGKF LPYSCKGKYP W
//
