ID A0A4D9DYN4_9SAUR Unreviewed; 675 AA.
AC A0A4D9DYN4;
DT 03-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 03-JUL-2019, sequence version 1.
DT 27-MAR-2024, entry version 14.
DE RecName: Full=sn-1-specific diacylglycerol lipase {ECO:0000256|ARBA:ARBA00026104};
DE EC=3.1.1.116 {ECO:0000256|ARBA:ARBA00026104};
GN ORFNames=DR999_PMT15605 {ECO:0000313|EMBL:TFK02097.1};
OS Platysternon megacephalum (big-headed turtle).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Testudinata; Testudines; Cryptodira; Durocryptodira;
OC Testudinoidea; Platysternidae; Platysternon.
OX NCBI_TaxID=55544 {ECO:0000313|EMBL:TFK02097.1, ECO:0000313|Proteomes:UP000297703};
RN [1] {ECO:0000313|EMBL:TFK02097.1, ECO:0000313|Proteomes:UP000297703}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DO16091913 {ECO:0000313|EMBL:TFK02097.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:TFK02097.1};
RA Gong S.;
RT "Draft genome of the big-headed turtle Platysternon megacephalum.";
RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:TFK02097.1, ECO:0000313|Proteomes:UP000297703}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DO16091913 {ECO:0000313|EMBL:TFK02097.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:TFK02097.1};
RA Gong S.;
RT "The genome sequence of big-headed turtle.";
RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty
CC acid + H(+); Xref=Rhea:RHEA:33275, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17389, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:28868; EC=3.1.1.116;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33276;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC {ECO:0000256|ARBA:ARBA00010701}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TFK02097.1}.
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DR EMBL; QXTE01000200; TFK02097.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4D9DYN4; -.
DR STRING; 55544.A0A4D9DYN4; -.
DR Proteomes; UP000297703; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR CDD; cd00519; Lipase_3; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002921; Fungal_lipase-like.
DR PANTHER; PTHR45792; DIACYLGLYCEROL LIPASE HOMOLOG-RELATED; 1.
DR PANTHER; PTHR45792:SF2; DIACYLGLYCEROL LIPASE-BETA; 1.
DR Pfam; PF01764; Lipase_3; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000297703};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 20..42
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 54..79
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 131..156
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 373..505
FT /note="Fungal lipase-like"
FT /evidence="ECO:0000259|Pfam:PF01764"
SQ SEQUENCE 675 AA; 75891 MW; FAEEFFE79B902CC6 CRC64;
MPGMVILTRR WAIASDDFVF PGAFELFIRM IWWIGILVLY SIHKGKFNCT GGGLLHSYLL
VLLILLAAII CAVSAIVYIS MQGTISNPGP RKSLPKLLYT RLGLYIPELV WAVVGAIWVS
DTDVRCERTV VNVVFWTVIA SWVIIIFTVV AVVIVFDPLG GKKTLYLADD INRNLESSQS
GQLFYNVKKT ATRVWETRIR LLCCCIMQDD DHRVAFTSIA ELFRTYFSDT DLVPSDIAAG
LTLLHQEQDK VENCPKEPEE VLCHSHLSHV TDDLDIELEN AAHYMQFAAA AYGWPYYIYT
NPCTALCKLN GDCCRNSPTD SDIIGGDRLN FHFGSILKIT GLQYRDFIHI SFHNKIFEIP
FFVALDHKKE AIVVAVRGTL SFEDLLTDLS AECEDLTLED VLENGFVHKG ITQAANYIYR
RLINDGILNQ AFTIAPEYKL VIVGHSLGGG TASILAIMLR NSFPTLRCYA FSPPGGLLSK
SLADYSKRFI VSVIVGKDLV ARLSMPNMED LKRRIVRIVA HCNRPKYQIL LYGCWYEVFG
GDPDNFPTEL DGRSQDALTQ PLLAEESLMM HRSPSYNAIE DESPLNAPPQ YPHLYLPGKI
IHIVEETSSR RWCSSDVKYT ARWSKETAFS NILISPKMIT DHMPDVVLKA LNNLTEEKAS
CITCQIEGDF HSNTV
//
