ID   A0A4P6ERU8_9BACL        Unreviewed;       421 AA.
AC   A0A4P6ERU8;
DT   31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT   31-JUL-2019, sequence version 1.
DT   27-MAR-2024, entry version 15.
DE   SubName: Full=Insulinase family protein {ECO:0000313|EMBL:QAY65642.1};
GN   ORFNames=ET464_03845 {ECO:0000313|EMBL:QAY65642.1};
OS   Paenibacillus protaetiae.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=2509456 {ECO:0000313|EMBL:QAY65642.1, ECO:0000313|Proteomes:UP000293568};
RN   [1] {ECO:0000313|EMBL:QAY65642.1, ECO:0000313|Proteomes:UP000293568}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FW100M-2 {ECO:0000313|EMBL:QAY65642.1,
RC   ECO:0000313|Proteomes:UP000293568};
RA   Heo J., Kim S.-J., Kim J.-S., Hong S.-B., Kwon S.-W.;
RT   "Genome sequencing of strain FW100M-2.";
RL   Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947};
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP035492; QAY65642.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A4P6ERU8; -.
DR   KEGG; pprt:ET464_03845; -.
DR   OrthoDB; 9811314at2; -.
DR   Proteomes; UP000293568; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 2.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   PANTHER; PTHR11851:SF149; GH01077P; 1.
DR   PANTHER; PTHR11851; METALLOPROTEASE; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 1.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 2.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000293568}.
FT   DOMAIN          11..157
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          165..338
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   421 AA;  47028 MW;  729464BBF49270F5 CRC64;
     MNKYTLSNGL RVVVEYIPHV RSVSFGIWVK TGSRNETPDN NGISHFIEHM LFKGTAKRSA
     KDIADLFDGI GGNVNAFTAK EYTCYFAKVL DEHLPIAVDA LSDMFFESVF DAGELAKEKN
     VILEEISMYE DTPDDKVHDE ASRAAFGDHP LAYSILGLEE RLTAMTSDTL RGYMKERYTI
     ENTVISVAGN VEEAKLLELL EKHFGGFSNH GIGSPVAAPD FKGDYIFFKK KSEQNHLCLS
     FTGCSNNDPN LYAMILLNNA LGGGMSSRLF QEIREKRGLA YSVYSYHTSY ADTGLFTIYA
     GTAPKQTQEV LDLTMEQLEE LAVKGLTEEE IHRGKEQLKG SLILSLESTS SRMNRLGKNE
     LMLGQHYTMD DLLQRVDAVT MADVQQMVEK MLRVPFSVAM VGTSDKPAAQ LRRDRFASRI
     V
//