ID   A0A4P7GQ42_9ACTN        Unreviewed;       457 AA.
AC   A0A4P7GQ42;
DT   31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT   31-JUL-2019, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   SubName: Full=Insulinase family protein {ECO:0000313|EMBL:QBR94149.1};
GN   ORFNames=EXE57_19045 {ECO:0000313|EMBL:QBR94149.1};
OS   Nocardioides euryhalodurans.
OC   Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC   Nocardioidaceae; Nocardioides.
OX   NCBI_TaxID=2518370 {ECO:0000313|EMBL:QBR94149.1, ECO:0000313|Proteomes:UP000294894};
RN   [1] {ECO:0000313|EMBL:QBR94149.1, ECO:0000313|Proteomes:UP000294894}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MMS17-SY117 {ECO:0000313|EMBL:QBR94149.1,
RC   ECO:0000313|Proteomes:UP000294894};
RA   Roh S.G., Lee C., Kim M.-K., Kim S.B.;
RT   "Three New Species of Nocardioides, Nocardioides euryhalodurans sp. nov.,
RT   Nocardioides seonyuensis sp. nov. and Nocardioides eburneoflavus sp. nov.,
RT   Iolated from Soil.";
RL   Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947};
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
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DR   EMBL; CP038267; QBR94149.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A4P7GQ42; -.
DR   KEGG; noy:EXE57_19045; -.
DR   OrthoDB; 9811314at2; -.
DR   Proteomes; UP000294894; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 2.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   PANTHER; PTHR11851:SF149; GH01077P; 1.
DR   PANTHER; PTHR11851; METALLOPROTEASE; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 1.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 2.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000294894}.
FT   DOMAIN          55..202
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          209..389
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   457 AA;  49132 MW;  F58DD11FC8D5E06E CRC64;
     MTSASTSRTT GRQLAAGRLS VDQTIGSTRT LETVRDESGQ VTSRVRRTVL PGGLRVITEQ
     LAGVRSASVG VWVGVGSRDE TPALHGCSHF LEHLLFKGTR SRSAMDISVA LDAVGGELNA
     FTAKEYTCFH ARVLDDDLPL AIDVLGDMVT DSQIRVEDVE AERDVILDEI AMHDDDPDDV
     VHNLFAEQAW GDTPLGRPIA GTVGSISALT RDQVARFYRR HYRAPGMVVA AAGNLDHTTV
     VRQVRKAFGR NGFLAGGDLP AVPRASSRVR RVHGGRTSAE RPFEQVNLVL GVNGLTRHDE
     RRYALGVLNA ALGGSTSSRL FQEVREHRGL AYSVYSFASH HADAGLVGVA VGCLPHRLDD
     VLETVRGELA KIAADGITDE ELERGKGQLR GGMVLGLEDS GSRMSRLGKA ELVYDELLSL
     DEVIARIDAV TRDDVRAVAA ALFRQPEILA VVGPGPA
//