ID   A0A4P9XDI0_9FUNG        Unreviewed;       934 AA.
AC   A0A4P9XDI0;
DT   31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT   31-JUL-2019, sequence version 1.
DT   24-JAN-2024, entry version 16.
DE   RecName: Full=Insulin-degrading enzyme {ECO:0008006|Google:ProtNLM};
DE   Flags: Fragment;
GN   ORFNames=CXG81DRAFT_9446 {ECO:0000313|EMBL:RKP03512.1};
OS   Caulochytrium protostelioides.
OC   Eukaryota; Fungi; Fungi incertae sedis; Chytridiomycota;
OC   Chytridiomycota incertae sedis; Chytridiomycetes; Caulochytriales;
OC   Caulochytriaceae; Caulochytrium.
OX   NCBI_TaxID=1555241 {ECO:0000313|EMBL:RKP03512.1, ECO:0000313|Proteomes:UP000274922};
RN   [1] {ECO:0000313|Proteomes:UP000274922}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 52028 {ECO:0000313|Proteomes:UP000274922};
RX   PubMed=30297742; DOI=10.1038/s41564-018-0261-0;
RA   Ahrendt S.R., Quandt C.A., Ciobanu D., Clum A., Salamov A.,
RA   Andreopoulos B., Cheng J.F., Woyke T., Pelin A., Henrissat B.,
RA   Reynolds N.K., Benny G.L., Smith M.E., James T.Y., Grigoriev I.V.;
RT   "Leveraging single-cell genomics to expand the fungal tree of life.";
RL   Nat. Microbiol. 3:1417-1428(2018).
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; ML014122; RKP03512.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A4P9XDI0; -.
DR   STRING; 1555241.A0A4P9XDI0; -.
DR   Proteomes; UP000274922; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   InterPro; IPR032632; Peptidase_M16_M.
DR   PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   Pfam; PF16187; Peptidase_M16_M; 1.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000274922};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          61..195
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          224..402
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          409..703
FT                   /note="Peptidase M16 middle/third"
FT                   /evidence="ECO:0000259|Pfam:PF16187"
FT   DOMAIN          721..893
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   REGION          1..41
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         934
FT                   /evidence="ECO:0000313|EMBL:RKP03512.1"
SQ   SEQUENCE   934 AA;  104566 MW;  4D3B887BBC87D03C CRC64;
     MVARSADASA APGAAITPTP AATRRRAPIP PSTTLSGLEK PTLDQREYRR VVLPNGLEAM
     LISDVHADRA GAALDVNIGY FQDPEDYPGL AHFLEHLLFL GTAKFPKENE YSAYLQDHSG
     WSNAYTDSEN TNYHFEVDAP AFEGAIDRFA QFFIAPLFDP SCTDRELKAV DSEHKKNLQA
     DAWRLQQVDA ELAAPEHPYH KFGTGSSETL KDRVSEDGQT VIPTRDRVMA FYKEYYSANL
     MRVALVGPQS LDTLESWLTT YFSPIPNQNL ALPSYVGQPF DARHVGRVVY AVPIRDEPYL
     SLTWQFDDQR PHYGKAGLQY LGDLLGQETK GSLLSLLKAK SYAHELFAGA DDSGRGFAFF
     NIQIGLTRRG AHHWVAVLAH AFQWIRMIQS HGVLRWYWDE CRDLADMSFR FSEAGAPGDY
     AVTVSNHMHL YAPNHVLSGS TLFREYDADH LGALLQHLTF DQCRVLLVSQ SLCDEAPPPP
     PPGWKILPWG YACQYQAQPC PPELRASIAM LKAHPDLHLP PPNPFIPNVP DEQGVPNPKL
     PQPTRPALVK ETPLYRLWHL TDQQFAVPKS VIHLYVRNPR VYGSVHMAAA HLMWIAALRD
     ALNETTYQAS LASVEVHIDA SLEGTVIVLS GYTEILPTVF ARIWAAMWAK PFTAPSHRWD
     AVKRHLIRNL RNTRYKSAQS FAVYSCLLAI QSALYMWNDK AAALEGITQS DVERLVHTSR
     EQLSMEVLIT GNMTAAASEA LIADALAQIR GPDTARPAPL PTSIRYALSR NHLIPTQRDW
     IYPLVLPNED DVNSAIEWYV QLSPGLTAKD RLAVQLFDQI TNEACFDVLR TQQQLGYLVH
     SGVRRHVGLI GFYVLIQSDR APTDLHDRIE AFIAGTPARI RALSPQAFRK HRESLAATLT
     TRERNLAEEH ARVWSAIYMG TWNFSVSHDD AAAL
//