ID A0A4Q7J8W7_9PSEU Unreviewed; 1214 AA.
AC A0A4Q7J8W7;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 08-NOV-2023, entry version 18.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN ORFNames=EWH70_12995 {ECO:0000313|EMBL:RZQ63366.1};
OS Amycolatopsis suaedae.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Amycolatopsis.
OX NCBI_TaxID=2510978 {ECO:0000313|EMBL:RZQ63366.1, ECO:0000313|Proteomes:UP000292003};
RN [1] {ECO:0000313|EMBL:RZQ63366.1, ECO:0000313|Proteomes:UP000292003}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=8-3EHSu {ECO:0000313|EMBL:RZQ63366.1,
RC ECO:0000313|Proteomes:UP000292003};
RA Duangmal K., Chantavorakit T.;
RT "Draft genome sequence of Amycolatopsis sp. 8-3EHSu isolated from roots of
RT Suaeda maritima.";
RL Submitted (FEB-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RZQ63366.1}.
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DR EMBL; SFCC01000006; RZQ63366.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4Q7J8W7; -.
DR OrthoDB; 9759518at2; -.
DR Proteomes; UP000292003; Unassembled WGS sequence.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000313|EMBL:RZQ63366.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000292003}.
FT DOMAIN 51..115
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
FT REGION 839..860
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1214 AA; 135104 MW; C75409F4581B064D CRC64;
MRRLTENVAS EALLRLGRYL TRWETSEDLH TVHRVGGRDA DGFYRDRWSH DKVVPSTHGV
NCTGSCRWQV YVSDGIITWE SQDPDYPSAG PDKPEYEPRG CPRGASFSWY SYSPTRVRFP
YARGVLVEMY REARRRLEDP VEAWKSVVTD PLKRRVYQRA RGKGGLVRIS WDEAAELAAA
AHVHAIAEYG PDRVAGFSPI PAMSLVSHAV GARFMSLIGA PMLSFYDWYA DLPVASPQVF
GDQTDVPESA DWWDAAYLMM WGSNVPVTRT PDAHWMVEAR YRGQKVVVVS PDYADNTKFA
DEWLAPHPGT DGALAMAMGH VLLTEFFVNS QVLYFLDYVE QYTDLPFLVT LEERDGAWVP
AKFLTEADLG GDGASPEWKT VLIDDRTGEP VVPGGSIGFR WNDADAGRWN LDLGDVEPRL
SMLDAADHTG VEVLLPRFDT PENDVLRRGV PAVEVAGHTV TTVYDLLLAQ YGVHRDDLPG
SWPSGYDDPS QPATPAWQQE ITSVPAERVT RIAREFAETA AATEGRCMIL MGAGTNHWFH
SDTIYRSFLA LLTLTGCQGR NGGGWAHYVG QEKVRPLTGH ATLAGAMDWV RPARQMIGTA
YWYLHTDQWR YDGLRHDTLL SPTGGGALAG TTAVDTFATS ARLGWMPSYP TFDRNPLDLV
DEAEAAGADP VEHAVEQLRK GRLGFAVDDP DAPENWPRVL TVWRSNLLGS SAKGHEYFLR
HLLGTDANLR ADETPHRPSV RWRDEAPEGK LDLLLCLDYR MTSTALFADV VLPAATWYEK
HDLSSTDMHP FVHSFNAAVD PPWQTRTDFD AFHTIARRFS ELAERHLGVR RDLVATPMVH
DTPGETAQPG GVVRDWRDGD VPPVPGVTMP RLTVVERDYP AVAAKLATLG PLLDEQGTTT
KGITYDVRAE LDWLRAKNGT SGGRPRIDTD IRAADAILAL SGTTNGRLAT QGFRTLGERV
GTELAGLSAE HEGKRISFED TRSRPASVVT SPEWSGSETG GRRYSAFVIN VEHRKPWHTL
TGRQHFFLDH DWMHELGEAM PIYRPPLDMH HIFGEPRIGP SGAEVTVRYL TPHSKWSIHS
TYQDNLHMLT LSRGGQAIWM SPADAEAIGA SDNDWIEAVN RNGIVVARAI VSHRMPTGTV
FLYHAQDRAV NVPRSETNGK RGGTHNSLTR LLMKPTHLIG GYAQLSFGFN YLGPTGNQRD
EVTVIRRRSQ EVQY
//
