ID A0A4Q7LFW5_9BURK Unreviewed; 610 AA.
AC A0A4Q7LFW5;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE SubName: Full=PQQ-dependent dehydrogenase (Methanol/ethanol family) {ECO:0000313|EMBL:RZS52218.1};
GN ORFNames=EV685_3409 {ECO:0000313|EMBL:RZS52218.1};
OS Sphaerotilus mobilis.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Sphaerotilaceae; Sphaerotilus.
OX NCBI_TaxID=47994 {ECO:0000313|EMBL:RZS52218.1, ECO:0000313|Proteomes:UP000293433};
RN [1] {ECO:0000313|EMBL:RZS52218.1, ECO:0000313|Proteomes:UP000293433}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 10617 {ECO:0000313|EMBL:RZS52218.1,
RC ECO:0000313|Proteomes:UP000293433};
RA Goeker M.;
RT "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the
RT most valuable type-strain genomes for metagenomic binning, comparative
RT biology and taxonomic classification.";
RL Submitted (FEB-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR617512-3};
CC Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR617512-3};
CC -!- COFACTOR:
CC Name=pyrroloquinoline quinone; Xref=ChEBI:CHEBI:58442;
CC Evidence={ECO:0000256|PIRSR:PIRSR617512-2};
CC Note=Binds 1 PQQ group per subunit. {ECO:0000256|PIRSR:PIRSR617512-2};
CC -!- SIMILARITY: Belongs to the bacterial PQQ dehydrogenase family.
CC {ECO:0000256|ARBA:ARBA00008156}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RZS52218.1}.
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DR EMBL; SGWV01000011; RZS52218.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4Q7LFW5; -.
DR Proteomes; UP000293433; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0016614; F:oxidoreductase activity, acting on CH-OH group of donors; IEA:InterPro.
DR CDD; cd10278; PQQ_MDH; 1.
DR Gene3D; 2.140.10.10; Quinoprotein alcohol dehydrogenase-like superfamily; 1.
DR InterPro; IPR018391; PQQ_beta_propeller_repeat.
DR InterPro; IPR017512; PQQ_MeOH/EtOH_DH.
DR InterPro; IPR002372; PQQ_repeat.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR NCBIfam; TIGR03075; PQQ_enz_alc_DH; 1.
DR PANTHER; PTHR32303:SF22; METHANOL DEHYDROGENASE LARGE SUBUNIT-LIKE PROTEIN; 1.
DR PANTHER; PTHR32303; QUINOPROTEIN ALCOHOL DEHYDROGENASE (CYTOCHROME C); 1.
DR Pfam; PF13360; PQQ_2; 1.
DR SMART; SM00564; PQQ; 4.
DR SUPFAM; SSF50998; Quinoprotein alcohol dehydrogenase-like; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837, ECO:0000256|PIRSR:PIRSR617512-3};
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR617512-4};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR617512-3};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW PQQ {ECO:0000256|PIRSR:PIRSR617512-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000293433};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..34
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 35..610
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5020293307"
FT ACT_SITE 332
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-1"
FT BINDING 87
FT /ligand="pyrroloquinoline quinone"
FT /ligand_id="ChEBI:CHEBI:58442"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-2"
FT BINDING 140
FT /ligand="pyrroloquinoline quinone"
FT /ligand_id="ChEBI:CHEBI:58442"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-2"
FT BINDING 184
FT /ligand="pyrroloquinoline quinone"
FT /ligand_id="ChEBI:CHEBI:58442"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-2"
FT BINDING 202
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-3"
FT BINDING 270
FT /ligand="pyrroloquinoline quinone"
FT /ligand_id="ChEBI:CHEBI:58442"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-2"
FT BINDING 290
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-3"
FT BINDING 332
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-3"
FT DISULFID 134..135
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-4"
SQ SEQUENCE 610 AA; 66577 MW; 5FE79D53990EF3B8 CRC64;
MTSTGNTAGK TGQWLIRGAC VALLGMPALT MANADVEKHI ANPKNWAMQA GDMYNQRYSQ
LKQINKSNVG KMQVAWTFST GVLRGHEGSP LVVDGTMYLH SPFPNKVFAI DLDTQKILWK
YEPKQDPAVI PQMCCDTVNR GLAYAEGKVF LQQADSNLIA LDAKSGKLVW TVKNGDPKLG
AVNTNAPHVF KDKVITGISG GEWGVRGFLA AYDIKTGKQV WKGYSVGPDS EMLMDPDKTT
TWADGKVTPV GRDSSLKTWQ GDQWKIGGGT TWGWYSYDKA TNAVYYGTGN PSTWNPAQRP
GDNKWTMSIW SRDVDSGKVN WVYQMTPYDE WDFDGINEMI LADINVKGKL TKALVHFDRN
GFAYTLDRVS GALLVAEKYD PKVNWATHVD MKSGRPQVVS KYSTAQNGPD VNTKGICPAA
LGSKDQQPAS YDPETKLFYV PTNHVCMDYE PFKVEYTAGQ PYVGATLSMF PAPGSHGGMG
NYITWDAGTG KIVQSKAEKF SVWSGSLNTA GGLSCYGTLE GYLKCVDKND INKELFKFKT
PSGIIGNVFT YEHKGKQYLG VFSGIGGWAG IGMAAGLEKD TDGLGAVGGY RELNQYTELG
GSLTVFALPN
//
