ID   A0A4Q9DTG8_9BACL        Unreviewed;       421 AA.
AC   A0A4Q9DTG8;
DT   31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT   31-JUL-2019, sequence version 1.
DT   27-MAR-2024, entry version 13.
DE   SubName: Full=Insulinase family protein {ECO:0000313|EMBL:TBL77778.1};
GN   ORFNames=EYB31_16690 {ECO:0000313|EMBL:TBL77778.1};
OS   Paenibacillus thalictri.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=2527873 {ECO:0000313|EMBL:TBL77778.1, ECO:0000313|Proteomes:UP000293142};
RN   [1] {ECO:0000313|EMBL:TBL77778.1, ECO:0000313|Proteomes:UP000293142}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N2SHLJ1 {ECO:0000313|EMBL:TBL77778.1,
RC   ECO:0000313|Proteomes:UP000293142};
RA   Tuo L.;
RT   "Paenibacillus sp. nov., isolated from surface-sterilized tissue of
RT   Thalictrum simplex L.";
RL   Submitted (FEB-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947};
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:TBL77778.1}.
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DR   EMBL; SIRE01000011; TBL77778.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A4Q9DTG8; -.
DR   OrthoDB; 9811314at2; -.
DR   Proteomes; UP000293142; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 2.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   PANTHER; PTHR11851:SF149; GH01077P; 1.
DR   PANTHER; PTHR11851; METALLOPROTEASE; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 1.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 2.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000293142}.
FT   DOMAIN          11..158
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          166..337
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   421 AA;  47147 MW;  07FFD0A87F1A7870 CRC64;
     MDKIKLSNGL RVVMEKIPTC RSVSFGIWVK TGSRNENELN NGISHFIEHM LFKGTDKYSA
     KDIAEIFDGI GGNVNAFTSK EYTCYYAKVL DEHLPIAVDV LSSMFFRSAF DEGEMEKEKN
     VIYEEISMYE DTPDDLVHDL LAKAAYGGNP LGYTIIGTEN NLSPMKPDDL RDYMKQHYHV
     DNTVISVAGN IDDSVIGLLE QHFGSFADVG QVHTASTPAF LGDAIFHEKK TEQNHICISF
     PGLSVTHDQL YSMVLLNNTI GGGMSSRLFQ EIREKRGLAY SVYSYHSSHY DSGLFTIYTG
     TAPKQTEEVL KVTMEMLNDI KENGITAAEL KKGKEQLKGS LILSLESTSS RMNRLGKNEL
     MLGKHYSMDE LIERIEAVQL ENVEELIHTI FNSPFAMATV GKVEQKIQDT RRDQLVSSSA
     N
//