ID A0A4Q9PRY9_9APHY Unreviewed; 305 AA.
AC A0A4Q9PRY9;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 24-JAN-2024, entry version 12.
DE RecName: Full=thymidylate synthase {ECO:0000256|ARBA:ARBA00011947};
DE EC=2.1.1.45 {ECO:0000256|ARBA:ARBA00011947};
GN ORFNames=BD310DRAFT_881460 {ECO:0000313|EMBL:TBU57166.1};
OS Dichomitus squalens.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Polyporales; Polyporaceae; Dichomitus.
OX NCBI_TaxID=114155 {ECO:0000313|EMBL:TBU57166.1, ECO:0000313|Proteomes:UP000292082};
RN [1] {ECO:0000313|EMBL:TBU57166.1, ECO:0000313|Proteomes:UP000292082}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 464.89 {ECO:0000313|EMBL:TBU57166.1,
RC ECO:0000313|Proteomes:UP000292082};
RG DOE Joint Genome Institute;
RA Lopez S.C., Andreopoulos B., Pangilinan J., Lipzen A., Riley R.,
RA Ahrendt S., Ng V., Barry K., Daum C., Grigoriev I.V., Hilden K.S.,
RA Makela M.R., de Vries R.P.;
RT "Draft genome sequences of three monokaryotic isolates of the white-rot
RT basidiomycete fungus Dichomitus squalens.";
RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-
CC dihydrofolate + dTMP; Xref=Rhea:RHEA:12104, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:57451, ChEBI:CHEBI:63528, ChEBI:CHEBI:246422;
CC EC=2.1.1.45; Evidence={ECO:0000256|ARBA:ARBA00001707};
CC -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004992}.
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DR EMBL; ML145141; TBU57166.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4Q9PRY9; -.
DR STRING; 114155.A0A4Q9PRY9; -.
DR UniPathway; UPA00575; -.
DR Proteomes; UP000292082; Unassembled WGS sequence.
DR GO; GO:0004799; F:thymidylate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006231; P:dTMP biosynthetic process; IEA:InterPro.
DR GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd00351; TS_Pyrimidine_HMase; 1.
DR Gene3D; 3.30.572.10; Thymidylate synthase/dCMP hydroxymethylase domain; 1.
DR HAMAP; MF_00008; Thymidy_synth_bact; 1.
DR InterPro; IPR045097; Thymidate_synth/dCMP_Mease.
DR InterPro; IPR023451; Thymidate_synth/dCMP_Mease_dom.
DR InterPro; IPR036926; Thymidate_synth/dCMP_Mease_sf.
DR InterPro; IPR000398; Thymidylate_synthase.
DR InterPro; IPR020940; Thymidylate_synthase_AS.
DR NCBIfam; TIGR03284; thym_sym; 1.
DR PANTHER; PTHR11548:SF2; THYMIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11548; THYMIDYLATE SYNTHASE 1; 1.
DR Pfam; PF00303; Thymidylat_synt; 1.
DR PRINTS; PR00108; THYMDSNTHASE.
DR SUPFAM; SSF55831; Thymidylate synthase/dCMP hydroxymethylase; 1.
DR PROSITE; PS00091; THYMIDYLATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Nucleotide biosynthesis {ECO:0000256|ARBA:ARBA00022727};
KW Reference proteome {ECO:0000313|Proteomes:UP000292082};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 12..305
FT /note="Thymidylate synthase/dCMP hydroxymethylase"
FT /evidence="ECO:0000259|Pfam:PF00303"
FT ACT_SITE 178
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10016"
SQ SEQUENCE 305 AA; 34534 MW; B81C1E2AF1A42603 CRC64;
MTVPATGPHD EHQYLNLIRS VLDSGDVRPD RTGTGTVSLF APPNLRFSLA DNTLPLLTTK
RTFLRGIVEE LLWFVCGSTD SKLLSAKGIK IWDGNGSKDF LDKRGLAHRR EGDLGPVYGF
QWRHFGAKYE SCDADYTGKG VDQLQECIRK IKNDPTDRRI ILSAWNPADI PEMALPPCHM
MCQFYVHLPP PDHPDAKPKL SCLMYQRSAD LGLGIPFNIA SYALLTHMVA HVTRTEPHEL
IIQLGDAHVY RDHIDALRTQ LEREPRPFPK LRWSRNVQNI DDFTSEDIIV EGYDPHPSIP
MKMSV
//
