ID A0A4Q9QD81_9APHY Unreviewed; 556 AA.
AC A0A4Q9QD81;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=tRNA (guanine(26)-N(2))-dimethyltransferase {ECO:0000256|ARBA:ARBA00039099, ECO:0000256|PROSITE-ProRule:PRU00958};
DE EC=2.1.1.216 {ECO:0000256|ARBA:ARBA00039099, ECO:0000256|PROSITE-ProRule:PRU00958};
GN ORFNames=BD310DRAFT_911960 {ECO:0000313|EMBL:TBU65737.1};
OS Dichomitus squalens.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Polyporales; Polyporaceae; Dichomitus.
OX NCBI_TaxID=114155 {ECO:0000313|EMBL:TBU65737.1, ECO:0000313|Proteomes:UP000292082};
RN [1] {ECO:0000313|EMBL:TBU65737.1, ECO:0000313|Proteomes:UP000292082}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 464.89 {ECO:0000313|EMBL:TBU65737.1,
RC ECO:0000313|Proteomes:UP000292082};
RG DOE Joint Genome Institute;
RA Lopez S.C., Andreopoulos B., Pangilinan J., Lipzen A., Riley R.,
RA Ahrendt S., Ng V., Barry K., Daum C., Grigoriev I.V., Hilden K.S.,
RA Makela M.R., de Vries R.P.;
RT "Draft genome sequences of three monokaryotic isolates of the white-rot
RT basidiomycete fungus Dichomitus squalens.";
RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) +
CC N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43140, Rhea:RHEA-COMP:10359, Rhea:RHEA-COMP:10360,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74513; EC=2.1.1.216;
CC Evidence={ECO:0000256|PROSITE-ProRule:PRU00958};
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Trm1 family. {ECO:0000256|PROSITE-ProRule:PRU00958}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; ML145084; TBU65737.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4Q9QD81; -.
DR STRING; 114155.A0A4Q9QD81; -.
DR Proteomes; UP000292082; Unassembled WGS sequence.
DR GO; GO:0160102; F:tRNA (guanine(10)-N2)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0160104; F:tRNA (guanine(26)-N2)-dimethyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.30.56.70; N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002905; Trm1.
DR InterPro; IPR042296; tRNA_met_Trm1_C.
DR NCBIfam; TIGR00308; TRM1; 1.
DR PANTHER; PTHR10631; N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR10631:SF3; TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE; 1.
DR Pfam; PF02005; TRM; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51626; SAM_MT_TRM1; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; Reference proteome {ECO:0000313|Proteomes:UP000292082};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00958};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU00958};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; tRNA processing {ECO:0000256|PROSITE-ProRule:PRU00958};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|PROSITE-
KW ProRule:PRU00958}.
FT REGION 60..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 532..556
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 60..92
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 556 AA; 61503 MW; 8F353C495FF678C7 CRC64;
MSAPAPTLIV PEGFTLHKEN TSAILLPASN DAFLNPVQEF NRDLSVACIR TWGDLMNKEK
EKKWRQSLER RAKKAEAGHK AKKAKTEENG HASEQPAEGA AVPQETSEGP PEFRPQRFVI
LEALSATGLR SIRYANEIPL VKYVIANDLS PAAVEAMRRN IELNDMHEKE VSPSESSSEK
VVRPAKVRVN EGDACALMYN HRAERNRVDV VDLDPYGTAA PFVDASVQCV NDGGLLCVTC
TDMSVLATTN YPEKCFSNYG GAPVKAEYCH EAALRLVLHT LATSAARYGR YIQPLLSLSI
DFYVRIFVRV YSSPIDVKKV FSKTAIYYVC TGCQSFYEQP IGRMVENVSG KGNVNLHFKA
HTGPPVSERC PECNSALHAA GPMWSGPLHD PEFVGSVLGH VESNEDKYGT SARMKGMLTE
LDTPFYFTPS RVSSHFHCVC PSLDETASAL LHAGYKVSRS HACAGSLKTT ATYADIHDIF
RSWVKTHPVR MDKISENSPA RHLLSKEPRI EANFKRHPDS VTPSSKVKLV RYQQNPTPNW
GPGAKPSGKR KRNDDD
//
