ID   A0A4Q9UYW5_9ACTO        Unreviewed;      1090 AA.
AC   A0A4Q9UYW5;
DT   31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT   31-JUL-2019, sequence version 1.
DT   24-JAN-2024, entry version 16.
DE   SubName: Full=HNH endonuclease {ECO:0000313|EMBL:TBW20918.1};
GN   ORFNames=EZJ44_07435 {ECO:0000313|EMBL:TBW20918.1};
OS   Arcanobacterium bovis.
OC   Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC   Arcanobacterium.
OX   NCBI_TaxID=2529275 {ECO:0000313|EMBL:TBW20918.1, ECO:0000313|Proteomes:UP000293036};
RN   [1] {ECO:0000313|EMBL:TBW20918.1, ECO:0000313|Proteomes:UP000293036}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C605018/01/1 {ECO:0000313|EMBL:TBW20918.1,
RC   ECO:0000313|Proteomes:UP000293036};
RA   Sammra O., Foster G., Hassan A., Alssahen M., Laemmler C., Borowiak M.,
RA   Malorny B., Abdulmawjood A.;
RT   "Arcanobacterium bovis sp. nov., isolated from the milk of a cow with
RT   mastitis.";
RL   Submitted (FEB-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:TBW20918.1}.
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DR   EMBL; SJDT01000006; TBW20918.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A4Q9UYW5; -.
DR   OrthoDB; 9802901at2; -.
DR   Proteomes; UP000293036; Unassembled WGS sequence.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.30.50; -; 1.
DR   Gene3D; 3.30.70.3520; -; 1.
DR   Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 2.
DR   InterPro; IPR040619; Cas9_alpha-helical_lobe.
DR   InterPro; IPR040796; Cas9_b_hairpin.
DR   InterPro; IPR041217; Cas9_C.
DR   InterPro; IPR041225; Cas9_Topo.
DR   InterPro; IPR002711; HNH.
DR   InterPro; IPR033114; HNH_CAS9.
DR   InterPro; IPR003615; HNH_nuc.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR041383; RuvC_III.
DR   Pfam; PF18470; Cas9_a; 1.
DR   Pfam; PF17893; Cas9_b_hairpin; 1.
DR   Pfam; PF18525; Cas9_C; 1.
DR   Pfam; PF17894; Cas9_Topo; 1.
DR   Pfam; PF01844; HNH; 1.
DR   Pfam; PF18541; RuvC_III; 1.
DR   SMART; SM00507; HNHc; 1.
DR   PROSITE; PS51749; HNH_CAS9; 1.
PE   4: Predicted;
KW   Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PROSITE-
KW   ProRule:PRU01085};
KW   Endonuclease {ECO:0000256|PROSITE-ProRule:PRU01085,
KW   ECO:0000313|EMBL:TBW20918.1};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU01085}; Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Nuclease {ECO:0000256|PROSITE-ProRule:PRU01085};
KW   Reference proteome {ECO:0000313|Proteomes:UP000293036};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT   DOMAIN          509..669
FT                   /note="HNH Cas9-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51749"
SQ   SEQUENCE   1090 AA;  121791 MW;  69D4EB1AC54791B0 CRC64;
     MKTMNYNVGV DVGTNSVGLC ALEIGENGLP TRILNSVVYV HDSGVDPEQR KKAITRLATS
     GVARRTRRLY RRRKQRLRAL DDFLTAKGYP IINLEEIADP HAPWHIRAKL AREELPADEF
     PSALSITLRH MARHRGWRSP YASVKSLHLP AADSEEFKKL KERLTEYNGV MPDEDATPAE
     IIADMIADYT KVRGEGGILG GKLRQSDNAN ELRKIAAKQG ISTEDINDII DVVFDAKSPV
     GSAASRTGKD ELPGQHHLPR AEKAHPAFQK YRIVSVVANL RIREDAGTTR SLTKDERLKV
     VEFLMEASSD DAVTWTDVAE LLGVVRDNLE GTAKEGPDGE RPYAKPPVNV TNQRIVKAKP
     KALSLWWQDA SEDERAALIA LMSNADILED DADGAEAAQS FLAGLSDKEL EELSGVDLPA
     GRAAYSVDSL TRLTNRMLNE GIDLHEARKA EFGVDDSWKP SADPIGLPVG NPGVDRVLKI
     VNRWLTSAIK EFGYPNVVNI EHMRDGFVSE KMAREYEREN GKRAERNRNI VEQIHADQGS
     GRVRKSDITR YLAVKRQNCQ CAYCGAQISM NDCEMDHIVP RKGVGSTNTR NNLAAVCISC
     NRSKGNIPFR VWAQQNPNPE ITLAKAVDRV KHWHYEEGLS TKQWNNFKKD VIDRLSRTSE
     DPELDNRSME SVAWMANELR HRIEYFLKQE NPNSKVGVYQ GKITAQARKA SGFEDRVEMI
     GGGGKTRLDR RHHAMDAATI ALMSPGISKT LAERINLRDT ERMLRKAETW KEYRGRFDGA
     KQSFGEWINK MDRLSFLFND ALATDSIPVM ENLRLGLGNG RAHDDSIRKL TYKEVGSAWT
     TTEIDRASSE ALWCALTRCE DFDAKTGLPE NDTRQIRVKN EYFTAKDTVG IFGTGAAAIA
     VRGGYAEIGN TIHHARVYRI NGKKPVFAMV RVFMCDLISH RGEDLFNVEL KPQSISIRAA
     EPKIRKALAD GTAEYLGWLV AGDEMVIDIS NPKLISGQIK EMLDDFPGQN RFRVAGFYSN
     ARLRLRPAML SAEGLKDQHC ESSYKIIDGG GWFPAINVIC QYGSPHVIRR SALGVPRTHS
     KHGLPESWHA
//