ID A0A4R4M8S0_9ACTN Unreviewed; 291 AA.
AC A0A4R4M8S0;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=4-hydroxy-tetrahydrodipicolinate synthase {ECO:0000313|EMBL:TDB91810.1};
GN ORFNames=E1264_00550 {ECO:0000313|EMBL:TDB91810.1};
OS Actinomadura sp. KC216.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Thermomonosporaceae; Actinomadura.
OX NCBI_TaxID=2530370 {ECO:0000313|EMBL:TDB91810.1, ECO:0000313|Proteomes:UP000294687};
RN [1] {ECO:0000313|EMBL:TDB91810.1, ECO:0000313|Proteomes:UP000294687}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KC216 {ECO:0000313|EMBL:TDB91810.1,
RC ECO:0000313|Proteomes:UP000294687};
RA Sahin N., Ay H., Saygin H.;
RT "Draft genome sequences of novel Actinobacteria.";
RL Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the DapA family.
CC {ECO:0000256|PIRNR:PIRNR001365}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TDB91810.1}.
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DR EMBL; SMJX01000002; TDB91810.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4R4M8S0; -.
DR OrthoDB; 9782828at2; -.
DR Proteomes; UP000294687; Unassembled WGS sequence.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR002220; DapA-like.
DR InterPro; IPR020625; Schiff_base-form_aldolases_AS.
DR InterPro; IPR020624; Schiff_base-form_aldolases_CS.
DR PANTHER; PTHR12128:SF72; 4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR12128; DIHYDRODIPICOLINATE SYNTHASE; 1.
DR Pfam; PF00701; DHDPS; 1.
DR PIRSF; PIRSF001365; DHDPS; 1.
DR PRINTS; PR00146; DHPICSNTHASE.
DR SMART; SM01130; DHDPS; 1.
DR SUPFAM; SSF51569; Aldolase; 1.
DR PROSITE; PS00665; DHDPS_1; 1.
DR PROSITE; PS00666; DHDPS_2; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|PIRNR:PIRNR001365};
KW Schiff base {ECO:0000256|ARBA:ARBA00023270}.
FT ACT_SITE 135
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001365-1"
FT ACT_SITE 163
FT /note="Schiff-base intermediate with substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001365-1"
FT BINDING 47
FT /ligand="pyruvate"
FT /ligand_id="ChEBI:CHEBI:15361"
FT /evidence="ECO:0000256|PIRSR:PIRSR001365-2"
FT BINDING 205
FT /ligand="pyruvate"
FT /ligand_id="ChEBI:CHEBI:15361"
FT /evidence="ECO:0000256|PIRSR:PIRSR001365-2"
SQ SEQUENCE 291 AA; 29993 MW; 05E6F4E617DB34CB CRC64;
MRAVHGIHAS LVTPFTRSGE VDVKSLERLA VHCLDGGADG LVALGTTGEA AMLSPAEQRT
VLEVCRAVSI EHGTPLTVGA GTIGTEDSIR QARERAPLAD ALLVVVPYYL RPSDDGVIDH
FAAIGSAVDV PLIAYHVPYR TGKPLPAGTL LRILALDCVA GIKQCAGAVD HDTLAVLAAH
TRKAVLCGDD AYLYPMLQLG ASGGVTASAC LAPAAYAAMA AATRDGNAPR ALALHNALLP
MADALFGEPS PSVLKACLVD EGLIDEPAVR APLHAPRPES VAAALRAFRA V
//
