ID A0A4S2MK77_9PEZI Unreviewed; 328 AA.
AC A0A4S2MK77;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 08-NOV-2023, entry version 12.
DE RecName: Full=Endonuclease {ECO:0000256|RuleBase:RU366055};
DE EC=3.1.30.- {ECO:0000256|RuleBase:RU366055};
GN ORFNames=EX30DRAFT_323669 {ECO:0000313|EMBL:TGZ77350.1};
OS Ascodesmis nigricans.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes;
OC Pezizales; Ascodesmidaceae; Ascodesmis.
OX NCBI_TaxID=341454 {ECO:0000313|EMBL:TGZ77350.1, ECO:0000313|Proteomes:UP000298138};
RN [1] {ECO:0000313|EMBL:TGZ77350.1, ECO:0000313|Proteomes:UP000298138}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 389.68 {ECO:0000313|EMBL:TGZ77350.1,
RC ECO:0000313|Proteomes:UP000298138};
RG DOE Joint Genome Institute;
RA Lutkenhaus R., Traeger S., Breuer J., Kuo A., Lipzen A., Pangilinan J.,
RA Dilworth D., Sandor L., Poggeler S., Barry K., Grigoriev I.V.,
RA Nowrousian M.;
RT "Comparative genomics and transcriptomics to analyze fruiting body
RT development in filamentous ascomycetes.";
RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|RuleBase:RU366055};
CC -!- SIMILARITY: Belongs to the DNA/RNA non-specific endonuclease family.
CC {ECO:0000256|ARBA:ARBA00010052, ECO:0000256|RuleBase:RU366055}.
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DR EMBL; ML220154; TGZ77350.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4S2MK77; -.
DR STRING; 341454.A0A4S2MK77; -.
DR InParanoid; A0A4S2MK77; -.
DR Proteomes; UP000298138; Unassembled WGS sequence.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR CDD; cd00091; NUC; 1.
DR Gene3D; 3.40.570.10; Extracellular Endonuclease, subunit A; 1.
DR InterPro; IPR018524; DNA/RNA_endonuclease_AS.
DR InterPro; IPR001604; DNA/RNA_non-sp_Endonuclease.
DR InterPro; IPR044929; DNA/RNA_non-sp_Endonuclease_sf.
DR InterPro; IPR020821; Extracellular_endonuc_su_A.
DR InterPro; IPR044925; His-Me_finger_sf.
DR InterPro; IPR040255; Non-specific_endonuclease.
DR PANTHER; PTHR13966:SF5; ENDONUCLEASE G, MITOCHONDRIAL; 1.
DR PANTHER; PTHR13966; ENDONUCLEASE RELATED; 1.
DR Pfam; PF01223; Endonuclease_NS; 1.
DR SMART; SM00892; Endonuclease_NS; 1.
DR SMART; SM00477; NUC; 1.
DR SUPFAM; SSF54060; His-Me finger endonucleases; 1.
DR PROSITE; PS01070; NUCLEASE_NON_SPEC; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759,
KW ECO:0000256|RuleBase:RU366055}; Hydrolase {ECO:0000256|RuleBase:RU366055};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR640255-2,
KW ECO:0000256|RuleBase:RU366055};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|RuleBase:RU366055};
KW Reference proteome {ECO:0000313|Proteomes:UP000298138}.
FT DOMAIN 79..297
FT /note="DNA/RNA non-specific endonuclease"
FT /evidence="ECO:0000259|SMART:SM00892"
FT DOMAIN 80..297
FT /note="Extracellular Endonuclease subunit A"
FT /evidence="ECO:0000259|SMART:SM00477"
FT ACT_SITE 144
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR640255-1"
FT BINDING 176
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR640255-2"
SQ SEQUENCE 328 AA; 35945 MW; 4715B8B353DD60D7 CRC64;
MSKFTALIAA ASAATGAACT AFFFSTRSQP APVTAAAPAS APVLSGSGSA AVKQALQSPV
NPAGLFQYGF PGPVNDLATR QALVSSFDRR TRNPIWVAEH ITAASLKMHG GDRKHSQFLE
DDAIPEKFRG KLKDYFRSGY DRGHQVPAAD AKFSQDAMND TFYLSNMCPQ VGDGFNRDYW
AHFEDFSRRL TQKYTSVRII TGPLYLPRLH PDGKYRVSYE VIGNPPNIAV PTHFYKVIIG
EGQLDAQGNA VGPVAVGAFV MPNAVIDNKT PLTEFVAPLE IVERAAGVEF MPLLKAERKK
ELCREVKCEI HVREFAKQVK GQQPVQKR
//
