ID A0A4S4C8A7_9BACL Unreviewed; 537 AA.
AC A0A4S4C8A7;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE SubName: Full=rRNA cytosine-C5-methyltransferase {ECO:0000313|EMBL:THF83884.1};
GN ORFNames=E6C55_04180 {ECO:0000313|EMBL:THF83884.1};
OS Cohnella fermenti.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Cohnella.
OX NCBI_TaxID=2565925 {ECO:0000313|EMBL:THF83884.1, ECO:0000313|Proteomes:UP000310636};
RN [1] {ECO:0000313|EMBL:THF83884.1, ECO:0000313|Proteomes:UP000310636}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-MHH1044 {ECO:0000313|EMBL:THF83884.1,
RC ECO:0000313|Proteomes:UP000310636};
RA Lin S.-Y., Hung M.-H., Young C.-C.;
RT "Cohnella sp. nov. isolated from preserved vegetables.";
RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|ARBA:ARBA00007494,
CC ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:THF83884.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; SSOB01000003; THF83884.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4S4C8A7; -.
DR OrthoDB; 9810297at2; -.
DR Proteomes; UP000310636; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd21147; RsmF_methylt_CTD1; 1.
DR Gene3D; 2.30.130.60; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR InterPro; IPR031341; Methyltr_RsmF_N.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR027391; Nol1_Nop2_Fmu_2.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR031340; RsmF_methylt_CI.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22807:SF30; 28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF17125; Methyltr_RsmF_N; 1.
DR Pfam; PF13636; Methyltranf_PUA; 1.
DR Pfam; PF17126; RsmF_methylt_CI; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS01153; NOL1_NOP2_SUN; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000310636};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 22..324
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT REGION 329..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 242
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 120..126
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 144
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 189
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 537 AA; 58199 MW; 83C7A3333AC8D9FD CRC64;
MRLPDKFKNT MRSLLEGEAD AFFATYAQPR TAGLRINGLK LGLTEYEERF RDDGRAGFGS
AGRTADPIPW AAGGVYIGEE DRPGKHLHYY LGLYYIQEPS AMLPAELLDV RPGHRVLDLC
AAPGGKSTQL AAKLAGRGLL VTNDNAAERT KALAKNTERA GMANAIVTNE EPAALAAAFG
DFFDRVLVDA PCSGEGMFRK DEDMIREWER HSVERCAAMQ EAILREAAKL VAPGGKLLYS
TCTFSPKENE LSVARLLSER PDFDVVPLEA PPEWGLAPGR PDWLAAEDTF GLPEERLAQL
AGTVRVWPHR ARGEGHYAAL LVRRPEPGAE AGESVAPAND GDWQFPPLPA PPAIAAAAKR
PGRGEASPRG KKPAGKGTAG KGPATSAEDD PRDKLLAFAK TTLPGWRPRG TLYRRGEQLY
LQPEELPDLG RLNAVRPGLL LGSATKHRFE PSQALAMGLR MEDAALSLRL RSGDGDALRY
MKGETLQPEP AAIVGTDGEP APGRGWTLVC IDGFPAGWGR WDGTILKNEL LPGWRWI
//
