ID A0A4T0X6Z3_9ASCO Unreviewed; 331 AA.
AC A0A4T0X6Z3;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 08-NOV-2023, entry version 12.
DE RecName: Full=Endonuclease {ECO:0000256|RuleBase:RU366055};
DE EC=3.1.30.- {ECO:0000256|RuleBase:RU366055};
GN ORFNames=CANINC_000708 {ECO:0000313|EMBL:TID30792.1};
OS [Candida] inconspicua.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Pichiaceae; Pichia.
OX NCBI_TaxID=52247 {ECO:0000313|EMBL:TID30792.1, ECO:0000313|Proteomes:UP000307173};
RN [1] {ECO:0000313|EMBL:TID30792.1, ECO:0000313|Proteomes:UP000307173}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 180 {ECO:0000313|EMBL:TID30792.1,
RC ECO:0000313|Proteomes:UP000307173};
RX PubMed=31105748; DOI=10.3389/fgene.2019.00383;
RA Mixao V., Hansen A.P., Saus E., Boekhout T., Lass-Florl C., Gabaldon T.;
RT "Whole-Genome Sequencing of the Opportunistic Yeast Pathogen Candida
RT inconspicua Uncovers Its Hybrid Origin.";
RL Front. Genet. 10:383-383(2019).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|RuleBase:RU366055};
CC -!- SIMILARITY: Belongs to the DNA/RNA non-specific endonuclease family.
CC {ECO:0000256|ARBA:ARBA00010052, ECO:0000256|RuleBase:RU366055}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TID30792.1}.
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DR EMBL; SELW01000121; TID30792.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4T0X6Z3; -.
DR STRING; 52247.A0A4T0X6Z3; -.
DR Proteomes; UP000307173; Unassembled WGS sequence.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR CDD; cd00091; NUC; 1.
DR Gene3D; 3.40.570.10; Extracellular Endonuclease, subunit A; 1.
DR InterPro; IPR018524; DNA/RNA_endonuclease_AS.
DR InterPro; IPR001604; DNA/RNA_non-sp_Endonuclease.
DR InterPro; IPR044929; DNA/RNA_non-sp_Endonuclease_sf.
DR InterPro; IPR020821; Extracellular_endonuc_su_A.
DR InterPro; IPR044925; His-Me_finger_sf.
DR InterPro; IPR040255; Non-specific_endonuclease.
DR PANTHER; PTHR13966:SF5; ENDONUCLEASE G, MITOCHONDRIAL; 1.
DR PANTHER; PTHR13966; ENDONUCLEASE RELATED; 1.
DR Pfam; PF01223; Endonuclease_NS; 1.
DR SMART; SM00892; Endonuclease_NS; 1.
DR SMART; SM00477; NUC; 1.
DR SUPFAM; SSF54060; His-Me finger endonucleases; 1.
DR PROSITE; PS01070; NUCLEASE_NON_SPEC; 1.
PE 3: Inferred from homology;
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759,
KW ECO:0000256|RuleBase:RU366055}; Hydrolase {ECO:0000256|RuleBase:RU366055};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR640255-2,
KW ECO:0000256|RuleBase:RU366055};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|RuleBase:RU366055};
KW Reference proteome {ECO:0000313|Proteomes:UP000307173};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..17
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 18..331
FT /note="Endonuclease"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5020685491"
FT DOMAIN 87..306
FT /note="DNA/RNA non-specific endonuclease"
FT /evidence="ECO:0000259|SMART:SM00892"
FT DOMAIN 88..306
FT /note="Extracellular Endonuclease subunit A"
FT /evidence="ECO:0000259|SMART:SM00477"
FT REGION 25..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..47
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..66
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 154
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR640255-1"
FT BINDING 186
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR640255-2"
SQ SEQUENCE 331 AA; 36653 MW; 169F591F23B4BADF CRC64;
MFKHGFVLPA VIPGASALFF WGGSSSSTST PTQAPPVPPP PPMGAPLGAP NNGKDTSTTK
STNNADDINP SAFFKYGFPG PVHDMGTHSE FVSVYDRQTR NPYYVVEHIT ADSIKKGDTN
GDRKNSVFKE DESIPVKFRA LLRDYFRSGY DRGHQAPAAD AKFSQTAMDE TFYLSNMSPQ
VGAGFNRDYW AHFEDFCRRL TGEYRSVRIV TGPLYLPKKC DDGKYRVTYE VIGNPPNIAV
PTHFFKLVVG EKSLKRNAGS EDIAVAAFVM PNAIIPNEVP LKSFQVPVES LERSSGLEFL
KLVPQKQVKE LCKEVSCEII VREFNNALPK K
//
