ID   A0A4U1C178_9SPHI        Unreviewed;       387 AA.
AC   A0A4U1C178;
DT   31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT   31-JUL-2019, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   SubName: Full=RsmB/NOP family class I SAM-dependent RNA methyltransferase {ECO:0000313|EMBL:TKB98971.1};
GN   ORFNames=FA046_07605 {ECO:0000313|EMBL:TKB98971.1};
OS   Pedobacter cryophilus.
OC   Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC   Sphingobacteriaceae; Pedobacter.
OX   NCBI_TaxID=2571271 {ECO:0000313|EMBL:TKB98971.1, ECO:0000313|Proteomes:UP000308181};
RN   [1] {ECO:0000313|EMBL:TKB98971.1, ECO:0000313|Proteomes:UP000308181}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AR-3-17 {ECO:0000313|EMBL:TKB98971.1,
RC   ECO:0000313|Proteomes:UP000308181};
RA   Dahal R.H., Kim D.-U.;
RT   "Pedobacter sp. AR-3-17 sp. nov., isolated from Arctic soil.";
RL   Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:TKB98971.1}.
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DR   EMBL; SWBP01000002; TKB98971.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A4U1C178; -.
DR   OrthoDB; 9810297at2; -.
DR   Proteomes; UP000308181; Unassembled WGS sequence.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR   InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR   InterPro; IPR023267; RCMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR22807:SF30; 28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED; 1.
DR   PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR   Pfam; PF01189; Methyltr_RsmB-F; 1.
DR   PRINTS; PR02008; RCMTFAMILY.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000308181};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW   ProRule:PRU01023};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01023};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}.
FT   DOMAIN          120..387
FT                   /note="SAM-dependent MTase RsmB/NOP-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51686"
FT   ACT_SITE        341
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         241
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         268
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         288
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ   SEQUENCE   387 AA;  44825 MW;  5130A6835839DD5B CRC64;
     MKAIHQLATF QNIIAQYPSD QPLSRFLAAF YRQNKQMGSK DRRIASRLLY NYFRLGNILK
     EESPQQRLIV AEFLVNNQPN SFLNHFKPEW DELVHLPLTE KIKIVEEQFP AFHLADVFPF
     SDHLTSVLDP PAFYLSFFIQ PDLYIRIKRG QEKGIINLVT DAGINFKIIN QQTLAFPNGT
     KLDQLIADKG IYEVQDISSQ KVGTYFKPNK WDKWWDCCAA SGGKSLLLHD EQPDIKLLVT
     DNRESILENL KERFALAGIR NYQLKILDLT LNQDQYLHDY VFDGIIYDAP CSGSGTWGRT
     PEMIAQFADH KIKFFTELQR KIAINVVKHL KPGKPIIYIT CSVFKEENEE QVAWLVKTFD
     LEVESQELIK GYQAKADTMF VARLLKR
//