ID A0A4V2JF85_9MICO Unreviewed; 449 AA.
AC A0A4V2JF85;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE SubName: Full=rRNA small subunit methyltransferase B {ECO:0000313|EMBL:TBN58529.1};
GN ORFNames=EYE40_04655 {ECO:0000313|EMBL:TBN58529.1};
OS Glaciihabitans arcticus.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Glaciihabitans.
OX NCBI_TaxID=2668039 {ECO:0000313|EMBL:TBN58529.1, ECO:0000313|Proteomes:UP000294194};
RN [1] {ECO:0000313|Proteomes:UP000294194}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RP-3-7 {ECO:0000313|Proteomes:UP000294194};
RA Dahal R.H.;
RT "Glaciihabitans arcticus sp. nov., a psychrotolerant bacterium isolated
RT from polar soil.";
RL Submitted (FEB-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|ARBA:ARBA00007494,
CC ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TBN58529.1}.
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DR EMBL; SISG01000001; TBN58529.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4V2JF85; -.
DR Proteomes; UP000294194; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.10.940.10; NusB-like; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR035926; NusB-like_sf.
DR InterPro; IPR006027; NusB_RsmB_TIM44.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22807:SF61; NOL1_NOP2_SUN FAMILY PROTEIN _ ANTITERMINATION NUSB DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF01029; NusB; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF48013; NusB-like; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS01153; NOL1_NOP2_SUN; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000294194};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 160..449
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT ACT_SITE 382
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 260..266
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 285
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 311
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 329
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 449 AA; 47958 MW; 7B987462E35B421C CRC64;
MQPARRIALD VILAVRESDA YANLLLPVRL QRAKLSTADA GFATELTYGT LRRQGYYDAV
IALAAGRTVD RIDAPILDVL RLACHQLLSM RTAQHAAVDE SVQLAKTVGS NSATGFVNGV
LRTITRSTAD EWRKRVLGDL DGDARLAAEF SHPLWIVRAF KAALAAEGRD SELENLLEAD
NVAPRVSLVA LPGFSTVEEL DAYPSPFSPV GATLDGGDPL QLEPVRAGRA RVQDEGSQLA
ALALSRARPV VAGETWLDLC AGPGGKSALL AAEARASGAV LTANELVPAR AELVRKALAV
FDPAPEVWEL DGTTIGDSHP DAFDRILIDA PCTGLGALRR RPEARWRKVP RDVAHLSGLQ
SNLLDAGLKA LKPGGVLAYV TCSPHLAETK VIVEGALKKW GAAVERIDTP AVLSTVTGEP
LDLGDGQHVQ LWPHRHSTDA MFIQLLTKV
//
