ID A0A4W3J2R3_CALMI Unreviewed; 1147 AA.
AC A0A4W3J2R3;
DT 18-SEP-2019, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2019, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE SubName: Full=Solute carrier family 12 member 5 {ECO:0000313|Ensembl:ENSCMIP00000033916.1};
GN Name=slc12a5 {ECO:0000313|Ensembl:ENSCMIP00000033916.1};
OS Callorhinchus milii (Ghost shark).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
OC Holocephali; Chimaeriformes; Callorhinchidae; Callorhinchus.
OX NCBI_TaxID=7868 {ECO:0000313|Ensembl:ENSCMIP00000033916.1, ECO:0000313|Proteomes:UP000314986};
RN [1] {ECO:0000313|Proteomes:UP000314986}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17185593; DOI=10.1126/science.1130708;
RA Venkatesh B., Kirkness E.F., Loh Y.H., Halpern A.L., Lee A.P., Johnson J.,
RA Dandona N., Viswanathan L.D., Tay A., Venter J.C., Strausberg R.L.,
RA Brenner S.;
RT "Ancient noncoding elements conserved in the human genome.";
RL Science 314:1892-1892(2006).
RN [2] {ECO:0000313|Proteomes:UP000314986}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17407382;
RA Venkatesh B., Kirkness E.F., Loh Y.H., Halpern A.L., Lee A.P., Johnson J.,
RA Dandona N., Viswanathan L.D., Tay A., Venter J.C., Strausberg R.L.,
RA Brenner S.;
RT "Survey sequencing and comparative analysis of the elephant shark
RT (Callorhinchus milii) genome.";
RL PLoS Biol. 5:E101-E101(2007).
RN [3] {ECO:0000313|Proteomes:UP000314986}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=24402279; DOI=10.1038/nature12826;
RG International Elephant Shark Genome Sequencing Consortium;
RA Venkatesh B., Lee A.P., Ravi V., Maurya A.K., Lian M.M., Swann J.B.,
RA Ohta Y., Flajnik M.F., Sutoh Y., Kasahara M., Hoon S., Gangu V., Roy S.W.,
RA Irimia M., Korzh V., Kondrychyn I., Lim Z.W., Tay B.H., Tohari S.,
RA Kong K.W., Ho S., Lorente-Galdos B., Quilez J., Marques-Bonet T.,
RA Raney B.J., Ingham P.W., Tay A., Hillier L.W., Minx P., Boehm T.,
RA Wilson R.K., Brenner S., Warren W.C.;
RT "Elephant shark genome provides unique insights into gnathostome
RT evolution.";
RL Nature 505:174-179(2014).
RN [4] {ECO:0000313|Ensembl:ENSCMIP00000033916.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(in) + K(+)(in) = chloride(out) + K(+)(out);
CC Xref=Rhea:RHEA:72427, ChEBI:CHEBI:17996, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034453};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR RefSeq; XP_007910490.1; XM_007912299.1.
DR AlphaFoldDB; A0A4W3J2R3; -.
DR Ensembl; ENSCMIT00000034429.1; ENSCMIP00000033916.1; ENSCMIG00000014337.1.
DR GeneTree; ENSGT00940000160827; -.
DR OrthoDB; 3641328at2759; -.
DR Proteomes; UP000314986; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015379; F:potassium:chloride symporter activity; IEA:InterPro.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR000076; KCL_cotranspt.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF54; SOLUTE CARRIER FAMILY 12 MEMBER 5; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 2.
DR Pfam; PF03522; SLC12; 3.
DR PRINTS; PR01081; KCLTRNSPORT.
PE 3: Inferred from homology;
KW Chloride {ECO:0000256|ARBA:ARBA00023214};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Potassium {ECO:0000256|ARBA:ARBA00022958};
KW Potassium transport {ECO:0000256|ARBA:ARBA00022538};
KW Reference proteome {ECO:0000313|Proteomes:UP000314986};
KW Symport {ECO:0000256|ARBA:ARBA00022847};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022847}.
FT TRANSMEM 140..162
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 174..197
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 218..239
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 272..291
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 298..317
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 436..456
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 476..502
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 600..622
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 634..658
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 145..317
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 437..715
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 729..845
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT DOMAIN 847..991
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT DOMAIN 1075..1147
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 25..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 965..1038
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..39
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 965..983
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 997..1015
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1016..1033
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1147 AA; 127596 MW; 1B1EAAC11F647A88 CRC64;
MSKRFIVTSI LADDGEDVLA QSNVAEMKTE SSVSNGNEPS EVIEHRDPSF LPAVEPPKPG
DGNPKESSPF INSTDPEKGK EYDGKNMALF EEEMDTIPMV SSLLSGLANY TNLTQGVKEH
EEAENNEGTR KKQVKATRMG TIMGVYLPCL QNIFGVILFL RLTWVVGIGG IIESFFIVLI
CCSCTMLTAI SMSAIATNGV VPAGGAYYMI SRSLGPEFGG AVGLCFYLGT TFAGAMYILG
TIEILLTYIV PQMAIFKAEE ASQQAGALLN NMRVYGTCVL SCMALIVFVG VKYVNKFALV
FLFCVIVSIL SIYAGVIKTA FEPPDFPVCI LGNRTLSRRD FDVCAKFITV GNETVTTQLW
RLFCSSGLLN ATCDEYFQQN NVTEIQGIPG VASGLITKNL WGNYLTKDVI VEKEGLPSIT
SSSTFNMDQP YVFTDMTSYF TLLVGIYFPS VTGIMAGSNR SGDLRDAQKS IPIGTIMAIT
TTSAVYVSTI ILFGACIEGV VLRDKFGEAV NGNLVIGTLS WPSPWVIVFG SLFSTCGAGL
QSLTGAPRLL QAIARDGIVP FLRIFGHGKV NGEPTWALLM TACICEIGIL IASLDEVAPI
LSMFFLMCYM FVNLACALQT LLRTPNWRPR FKYYHWTLSF LGMSLCLSLM FICSWYYAIV
AMVIAGLIYK YIEFNGAEKE WGDGIRGISL SAARYALLRL EEGPPHTKNW RPQLLVLVKV
DQDQNVKHPQ LLTFTCQLKA GKGLTIVGSV LEGSFLENHT QAQRAEESIR RLMEVEKVKG
FCQVVISSNL RDGMSHLIQS CGLGGMQHNT VLMGWPRAWR QSEDPKTWKN LIEVVRETTA
GHLALLVAKN ITAFPSNQER FAEGNIDVWW IVHDGGMLML LPFLLRQHKV WRKCKMRIFT
VAQMEDNSIQ MKKDLTVFLY HLRIQAEVEV VEMHDSDISA YTYERTLMME QRSQILKEMH
LTKNEREREI QSITDESRGS IRRKHPANAK LHLSVQDEQE GSSEEKPEEE VQLIHDKNAS
TLSIPSQSPA DEAGTPSEKV HMTWTKEKFT ADKNKNRGPI TPEEIRDLFS MKPNQSNVRR
MHTAVKLNEV IVKKSQDAKL VLMNMPGPPK NRKGDENYME FLEVLTEGLD RVLLVRGGGR
EVITIYS
//
