ID A0A4W4H6E7_ELEEL Unreviewed; 2328 AA.
AC A0A4W4H6E7;
DT 18-SEP-2019, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE SubName: Full=Trio Rho guanine nucleotide exchange factor a {ECO:0000313|Ensembl:ENSEEEP00000047050.1};
GN Name=trioa {ECO:0000313|Ensembl:ENSEEEP00000047050.1};
OS Electrophorus electricus (Electric eel) (Gymnotus electricus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Gymnotiformes;
OC Gymnotoidei; Gymnotidae; Electrophorus.
OX NCBI_TaxID=8005 {ECO:0000313|Ensembl:ENSEEEP00000047050.1, ECO:0000313|Proteomes:UP000314983};
RN [1] {ECO:0000313|Proteomes:UP000314983}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=24970089;
RA Gallant J.R., Traeger L.L., Volkening J.D., Moffett H., Chen P.H.,
RA Novina C.D., Phillips G.N.Jr., Anand R., Wells G.B., Pinch M., Guth R.,
RA Unguez G.A., Albert J.S., Zakon H.H., Samanta M.P., Sussman M.R.;
RT "Nonhuman genetics. Genomic basis for the convergent evolution of electric
RT organs.";
RL Science 344:1522-1525(2014).
RN [2] {ECO:0000313|Proteomes:UP000314983}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=28695212;
RA Traeger L.L., Sabat G., Barrett-Wilt G.A., Wells G.B., Sussman M.R.;
RT "A tail of two voltages: Proteomic comparison of the three electric organs
RT of the electric eel.";
RL Sci. Adv. 3:e1700523-e1700523(2017).
RN [3] {ECO:0000313|Ensembl:ENSEEEP00000047050.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR Ensembl; ENSEEET00000047571.1; ENSEEEP00000047050.1; ENSEEEG00000022032.1.
DR GeneTree; ENSGT00940000154766; -.
DR Proteomes; UP000314983; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR CDD; cd13240; PH1_Kalirin_Trio_like; 1.
DR CDD; cd13241; PH2_Kalirin_Trio_p63RhoGEF; 1.
DR CDD; cd00160; RhoGEF; 2.
DR CDD; cd00170; SEC14; 1.
DR CDD; cd11852; SH3_Kalirin_1; 1.
DR CDD; cd00176; SPEC; 6.
DR Gene3D; 1.20.58.60; -; 5.
DR Gene3D; 3.40.525.10; CRAL-TRIO lipid binding domain; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 2.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 2.
DR Gene3D; 2.30.30.40; SH3 Domains; 2.
DR InterPro; IPR001251; CRAL-TRIO_dom.
DR InterPro; IPR036865; CRAL-TRIO_dom_sf.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR047054; Kalirin_TRIO_PH_1.
DR InterPro; IPR028570; Kalirin_TRIO_SH3_1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR InterPro; IPR018159; Spectrin/alpha-actinin.
DR InterPro; IPR002017; Spectrin_repeat.
DR PANTHER; PTHR22826; RHO GUANINE EXCHANGE FACTOR-RELATED; 1.
DR PANTHER; PTHR22826:SF206; TRIPLE FUNCTIONAL DOMAIN PROTEIN-RELATED; 1.
DR Pfam; PF13716; CRAL_TRIO_2; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00621; RhoGEF; 2.
DR Pfam; PF16609; SH3-RhoG_link; 1.
DR Pfam; PF00018; SH3_1; 1.
DR Pfam; PF00435; Spectrin; 4.
DR SMART; SM00233; PH; 2.
DR SMART; SM00325; RhoGEF; 2.
DR SMART; SM00516; SEC14; 1.
DR SMART; SM00326; SH3; 1.
DR SMART; SM00150; SPEC; 7.
DR SUPFAM; SSF52087; CRAL/TRIO domain; 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 2.
DR SUPFAM; SSF50729; PH domain-like; 2.
DR SUPFAM; SSF50044; SH3-domain; 1.
DR SUPFAM; SSF46966; Spectrin repeat; 6.
DR PROSITE; PS50191; CRAL_TRIO; 1.
DR PROSITE; PS50010; DH_2; 2.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50002; SH3; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000314983};
KW SH3 domain {ECO:0000256|ARBA:ARBA00022443, ECO:0000256|PROSITE-
KW ProRule:PRU00192}.
FT DOMAIN 1..148
FT /note="CRAL-TRIO"
FT /evidence="ECO:0000259|PROSITE:PS50191"
FT DOMAIN 1227..1402
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
FT DOMAIN 1414..1526
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 1590..1655
FT /note="SH3"
FT /evidence="ECO:0000259|PROSITE:PS50002"
FT DOMAIN 1904..2080
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
FT REGION 1536..1585
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1685..1842
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1859..1890
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2238..2284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 689..719
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 813..840
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1536..1561
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1562..1585
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1741..1774
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1775..1790
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1814..1842
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1866..1890
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2328 AA; 264803 MW; D6B6A346BC05BEA8 CRC64;
KKSLSYIPLH PVDFPCGRDK RGGPVLTFPS RTNHDRIRQE DLRRLIAYLA GIPSEDVCKH
GFTVIVDMRG SKWDSIKPLL KILQESFPCC IHVALIIKPD NFWQKQRTNF GSSKFEFETT
MVSLEGLSKV VDPSQLTPDF EGSLDYNHEE WIEIRVAFED FTGNARHLLS RLEEMQATVT
RKDFPQDLDG ARRMIEEHAA LKKKVMKAPV EEVDGEGQCL LQRIQSSESY GTGQANADTQ
GLVPRITTLL EQLHATQQAL HQAWHVRKLQ LDQCFQLRLF EQDAEKMFDW IMHNKGLFLA
GYTEIGNNHS HAMELQTQHN HFAMNCMNVY VNINRIMSVG NRLLEAGHYA SQQIKQISSQ
LEQEWKAFAA ALDERSTLLE MSAAFHQKCD QYMSNVDSWC KACGEVDLPS ELQDLEDAIH
HHQGLYEHIS TAYSEVSQDG KALLDKLQRP LTPGSADSLT ASANYSKAVH HVLDIIHEVL
HHQRQLENIW QHRKVRLHQR LQLCVFQQDV QQVLDWIENH GEAFLSKHTG VGKSLHRARA
LQKRHEDFEE VAQNTYTNAD KLLEAAEQLA QTGECDPEEI YQAAHQLEDR IQDFVRRVEQ
RKVLLDMSVA FHTHVKELWT WLEELQKELL DDVYAESVEA VQDLIKRFGQ QQQTTLQVTV
NVIKEGEDLI QQLRDSAISS NKAPHNSSIN HIESVLQQLD EAQAQMEELF QERKIKLELF
LQLRIFERDA IDIISDLDSW NEELSQQMSD FDTEDLTLAE QRLQHHADKA LTMNNLTFDV
IHQGQELLQY VNEVQASGVE LLCDRDVDMA TRVQDLLEFL HEKQQELDAA AEQHRRHLEQ
CVQLRHLQAE VKQVLGWIRN GESMLNAGLI TASSLQEAEQ LQREHEQFQH AIEKTHQSAL
QVQQKAEALL QANHYDMDVI RDCAENVASH WQQLMLKMED RLKLVNASVA FYKTSEQVCS
VLESLEQEYK RDEDWCGGAD KLGPNCETDH VTPMISKHLE QKEAFLKACT LARRNADVFL
KYMHRNSVNM PGMLSHVKAP EQQVKNILNE LLQRENRVLH FWTMRKRRLD QCQQYVVFER
SAKQALEWIH DTGEFYLSTH TSTGSSIHHT QELLKEHEDF HITAKQTKER VKLLIQLADG
FCDKGHAHAT EIKKWVTAVD KRYRDFSLRM DKYRMSLEKA LGISCDSNKA SKDLQLDIIP
ASTPGSEVKL RDAAHELNEE KRKSARRKDF IMAELIQTEK AYVRDLRECM DTYLWEMTSG
VEEIPPGIVN KEHIIFGNMQ DLYEFHHNIF LKELEKYEQL PEDVGHCFVT WADKFQMYVN
YCKNKPDSTQ LILELAGSYF DEIQQRHRLA NSISSYLIKP VQRITKYQLL LKELLTCCEE
GKGEIKDGLE VMLSVPKRAN DAMHLSMLEG FDENIESQGE LILQESFQVW DPKTLIRKGR
ERHLFLFEMS LVFSKEVKDS NGRSKYIYKS KLFTSELGVT EHVEGDPCKF ALWVGRTPTS
DNKIVLKASS IENKQDWIKH IREVIQERTV HLKGALKEPI HIPKPSTTKH KGRRDGEDLD
SQGDGSSQPD TISLASRTSQ NTLDSDKLSG GCELTVVIHD FMPANSNELL VRRGQTVEVL
ERLHDKPDWC LVRTTDRSPA QEGLVPCATL CLAHSRSSME MEGIFNHKDT LSVCSNDAIL
PGSSATLQPS HVMGSHSSPG PKRPGNTLRK WLTSPVRRLS SGKADGHVKK LAHKHKKSRD
VRKSADVGSQ KDSDDSAATP QDETLEERVR HEGLSSGTLS KSSSSGMQSC GEEEGEEGPD
AVPLPPPMAI QQHSLLQPDP QDDKTSSRLS VRPPSSETPS AAELVSAIEE LVKSKMALED
RPSSLCVEQG DSSSPSFNPS DNSLLSSSSP IDELDERKTT FLKKRHYVLL ELIETERDYV
RDLGLVVEGY MARMREEGVP DDMKGKDKIV FGNIHQIYDW HRDFFLGELE KCLEDPDHLG
LLFMKHERRL HMYIVYCQNK PKSEHIVSEY IDTYFEDQKQ RLGHKLQITD LLIKPVQRIM
KYQLLLKDFL KFSKKAGLDS LDLEKAVEVM CIVPKRCNDM MNVGRLQGFD GKIVAQGRLL
LQDTFMVSDS DGGLLGRMKE RRVFLFEQIV IFSEPLDKKR GFTMPGFLYN NSIKVSCLGL
EDSVDGDPCK FTLTSRMSTG STEAFVLHSS HPGVRQVWTL QISQILESQR NFFTALTSPI
EYQKNHVGGS GSVSVGSAGG AHAGTGGGQG IVPGGGGIGS AGSGPRSRPS RIPQPSRLPQ
PLRHHSPSMF LVFRAATEQS PAAEGWIPGY VLGHTSTSIP DTPEGTIK
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