ID A0A4W6EKC0_LATCA Unreviewed; 1076 AA.
AC A0A4W6EKC0;
DT 18-SEP-2019, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2019, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE SubName: Full=Solute carrier family 12 member 4 {ECO:0000313|Ensembl:ENSLCAP00010038645.1};
GN Name=SLC12A4 {ECO:0000313|Ensembl:ENSLCAP00010038645.1};
OS Lates calcarifer (Barramundi) (Holocentrus calcarifer).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Carangaria; Carangaria incertae sedis; Centropomidae; Lates.
OX NCBI_TaxID=8187 {ECO:0000313|Ensembl:ENSLCAP00010038645.1, ECO:0000313|Proteomes:UP000314980};
RN [1] {ECO:0000313|Proteomes:UP000314980}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Sai Rama Sridatta P.;
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLCAP00010038645.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(in) + K(+)(in) = chloride(out) + K(+)(out);
CC Xref=Rhea:RHEA:72427, ChEBI:CHEBI:17996, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034453};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR AlphaFoldDB; A0A4W6EKC0; -.
DR Ensembl; ENSLCAT00010039562.1; ENSLCAP00010038645.1; ENSLCAG00010017027.1.
DR GeneTree; ENSGT00940000157672; -.
DR Proteomes; UP000314980; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015379; F:potassium:chloride symporter activity; IEA:InterPro.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR000076; KCL_cotranspt.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF46; SOLUTE CARRIER FAMILY 12 MEMBER 4; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 2.
DR Pfam; PF03522; SLC12; 2.
DR PRINTS; PR01081; KCLTRNSPORT.
PE 3: Inferred from homology;
KW Chloride {ECO:0000256|ARBA:ARBA00023214};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Potassium {ECO:0000256|ARBA:ARBA00022958};
KW Potassium transport {ECO:0000256|ARBA:ARBA00022538};
KW Reference proteome {ECO:0000313|Proteomes:UP000314980};
KW Symport {ECO:0000256|ARBA:ARBA00022847};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022847}.
FT TRANSMEM 21..39
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 124..143
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 155..180
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 200..225
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 253..270
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 276..295
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 456..482
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 494..513
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 555..574
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 581..602
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 614..640
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 124..295
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 395..695
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 709..827
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT DOMAIN 841..1076
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
SQ SEQUENCE 1076 AA; 120130 MW; 6030D0364C6F7835 CRC64;
MEIMSHFTSF ENLDNFDLPK NNFFLLLTLS CFFLFPIGHG NHKEDSPFLN SAHAANKKND
FYDRNLALFE EELDIRPKVS SLLSRLVNYT NITQGAKEHE EEESAEASRR KTPKSPNMGT
LMGVYLPCLQ NIFGVILFLR LTWIVGMAGI MQSLLIVLMC CSCTMLTAIS MSAIATNGVV
PAGGAYFMIS RSLGPEFGGA VGLCFYLGTT FASAMYILGA IEIFLKYLVP QAAIFHGTDP
LGSDSAMLNN MRVYGSICLT LMAVVVFVGV RYVNKLASLF LACVIISIVS IYAGAIKSMT
HPPEFPICML GNRTLVRDRF DVCAKTVIQG NITVPSQLWE RFCLPGNMSS AQCDDYFIQN
NVTEIQGIPG LGSGIIRENM WGNYLQKGEI LEKAGLQSVD THGALDNFGM YVSADIATSF
TLLVGIFFPS ATGIMAGSNR SGDLRDAQKS IPVGTILAIT TTSLVYLSSV VLFGSCIEGV
VLRDKFGDAV RKNLVVGTLS WPSPWVIVIG SFFSTVGAGL QSLTGAPRLL QAIAKDNIIP
FLRVFGHGKT NGEPTWALLL TGLIAELGIL IASLDMVAPI LSMFFLMCYL FVNLACAVQT
LLRTPNWRPR FKYYHWALSF LGMSMCLALM FISSWYYAIV AMGIAGMIYK YIEYQGAEKE
WGDGIRGLSL SAARYALLRL EVGPPHTKNW RPQLLVLLKL DEDLHVKYPR LLTFASQLKA
GKGLTIVGSV IQGNFLDSYG EMQAAEQAIK NMMEIERVKG FCQVVVASKV REGIVHLIQS
CGLGGMKHNT VVMGWPYGWR QSEDPRAWKT FINTVRCTTA AHLALMVPKN VSFYPSNHER
FTDGNIDVWW IVHDGGMLML LPFLLKQHKV WRKCKMRIFT VAQMDDNSIQ MKKDLATFLY
QLRIEAEVEV VEMHDSDISA YTYERTLMME QRSQMLRQMR LSSAERQREA QLVKDRHSLV
RMGSLYSDEE EEVVEAPPEK VQMTWTKERP TLLCLSFLLQ PRDQSNVRRM HTAVKLNEVI
VNRSHDARLV LLNMPGPPRN ADGDENYMEF LEVLTEGLER VLLVRGGGRE VITIYS
//
