ID A0A4W6G8A9_LATCA Unreviewed; 1138 AA.
AC A0A4W6G8A9;
DT 18-SEP-2019, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2019, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Solute carrier family 12 member 2 {ECO:0000313|Ensembl:ENSLCAP00010059749.1};
GN Name=LOC108881529 {ECO:0000313|Ensembl:ENSLCAP00010059749.1};
OS Lates calcarifer (Barramundi) (Holocentrus calcarifer).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Carangaria; Carangaria incertae sedis; Centropomidae; Lates.
OX NCBI_TaxID=8187 {ECO:0000313|Ensembl:ENSLCAP00010059749.1, ECO:0000313|Proteomes:UP000314980};
RN [1] {ECO:0000313|Proteomes:UP000314980}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Sai Rama Sridatta P.;
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLCAP00010059749.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 chloride(out) + K(+)(out) + Na(+)(out) = 2 chloride(in) +
CC K(+)(in) + Na(+)(in); Xref=Rhea:RHEA:72395, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72396;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR AlphaFoldDB; A0A4W6G8A9; -.
DR Ensembl; ENSLCAT00010061366.1; ENSLCAP00010059749.1; ENSLCAG00010027711.1.
DR GeneTree; ENSGT00940000155742; -.
DR Proteomes; UP000314980; Unassembled WGS sequence.
DR GO; GO:0098590; C:plasma membrane region; IEA:UniProt.
DR GO; GO:0015377; F:chloride:monoatomic cation symporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR002444; NKCC1.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002443; SLC12A1/SLC12A2.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF58; SOLUTE CARRIER FAMILY 12 MEMBER 2; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 1.
DR PRINTS; PR01207; NAKCLTRNSPRT.
DR PRINTS; PR01208; NAKCLTRSPRT1.
PE 3: Inferred from homology;
KW Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000314980};
KW Sodium {ECO:0000256|ARBA:ARBA00023053};
KW Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT TRANSMEM 233..266
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 286..307
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 328..347
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 353..374
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 405..423
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 435..454
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 572..591
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 597..616
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 628..648
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 654..671
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 142..195
FT /note="Amino acid permease N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08403"
FT DOMAIN 237..712
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 721..1138
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 1..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 876..929
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 55..74
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 120..138
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 900..916
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1138 AA; 124044 MW; 5ECEBCCAC70C8C3C CRC64;
MSAPSSSPSA PAENVATEGD FLAPDAGQRP AGPTPSQSRF QVDLVAEAAG ATEDKSPNSD
ASSTVPSSDK ATPATPQDAD PGTGGEEAKG RFRVVNFADP SGSGSAASPE AAPAEGLQNG
DTVMSETSLH SSTGGQHHYH YDTHTNTYYL RTFGHNTIDA VPNIDFYRQT AAPLGEKLIR
PTLSELHDEL DKEPFEDGFA NGDELTPAEE AAAKEAAESK GVVKFGWIKG VHYIFHFLSC
VIVAMATVVT TITGLSTSAI ATNGFVRGGG AYYLISRSLG PEFGGSIGLI FAFANAVAVA
MYVVGFAETV VELLAGVDAI MTDQINDIRI VGTITVIVLL GISVAGMEWE AKAQIFLLVV
LITAIVNFFI GTFIPSKSKE SVGFFGYDGA IMWENMGPDF RGESFFSVFA IFFPAATGIL
AGANISGDLA DPQLAIPRGT LLAILITGIV YLGVAISTGS CIVRDATGNV NDTLSAQFMN
CTDAACKFGY DFSACKSEKG SCKYGLHNDF QVMSLVSGFG PIISAGIFSA TLSSALASLV
SAPKVFQALC KDNIYPGLGM FAKGYGKNNE PLRGYILTFG IALAFILIAE LNTIAPIISN
FFLASYALIN FSVFHASLAN SPGWRPSFIY YNMWVSLAGA ILCCVVMFVI NWWAALLTNV
IVLGLYIYVS YKKPDVNWGS STQALTYHQA LTHTLHLSGV EDHIKNFRPQ CLVMTGYPNS
RPALLDLVHS FTKNVGLMIC GHVRAGYRRP NFKDLATDQS RYQRWLLKNE TKAFYTPVFS
EDMRQGTQYL LQAAGLGRLK PNTLVLGFKN DWRDGDMMNV ETYISMIHDA FDFQFGAVIL
RLKEGLDVSH IQGQDELLSS QEKSSGMKDV IVSIDTSKDS DADSSKPSSK ATSLQNSPAI
QKDDDDDGKA TTQPLLKKDK RSPTVPLNVS DQRLLEASQQ FQRKQGKGTV DVWWLFDDGG
LTLLIPYLLT NKKRWKDCKI RVFIGGKINR IDHDRRAMAT LLSKFRIDFS DITVLGDINT
KPKKEHMVAF EEMIEPYRLK EDDMEQEAAE RLKNSEPWRI TDNELELYRA KTNRQIRLNE
LLKEHSSTAN LIVMSLPLAR KGAVSSALYM AWLEALSKDL PPILLVRGNH QSVLTFYS
//
