ID A0A4Y8DER1_9HELO Unreviewed; 718 AA.
AC A0A4Y8DER1;
DT 18-SEP-2019, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2019, sequence version 1.
DT 24-JAN-2024, entry version 14.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=BOTCAL_0038g00210 {ECO:0000313|EMBL:TEY80423.1};
OS Botryotinia calthae.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Helotiales; Sclerotiniaceae; Botryotinia.
OX NCBI_TaxID=38488 {ECO:0000313|EMBL:TEY80423.1, ECO:0000313|Proteomes:UP000297299};
RN [1] {ECO:0000313|EMBL:TEY80423.1, ECO:0000313|Proteomes:UP000297299}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MUCL2830 {ECO:0000313|EMBL:TEY80423.1,
RC ECO:0000313|Proteomes:UP000297299};
RA Valero-Jimenez C.A., Tapia P., Veloso J., Silva-Moreno E., Staats M.,
RA Valdes J.H., Van Kan J.A.L.;
RT "Comparative genomics of Botrytis spp.";
RL Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TEY80423.1}.
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DR EMBL; PHWZ01000038; TEY80423.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4Y8DER1; -.
DR STRING; 38488.A0A4Y8DER1; -.
DR Proteomes; UP000297299; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 3.40.970.10; Ribonuclease H1, N-terminal domain; 1.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR InterPro; IPR009027; Ribosomal_bL9/RNase_H1_N.
DR InterPro; IPR011320; RNase_H1_N.
DR InterPro; IPR037056; RNase_H1_N_sf.
DR PANTHER; PTHR47642; ATP-DEPENDENT DNA HELICASE; 1.
DR PANTHER; PTHR47642:SF5; ATP-DEPENDENT DNA HELICASE; 1.
DR Pfam; PF01693; Cauli_VI; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF55658; L9 N-domain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000297299}.
FT DOMAIN 10..51
FT /note="Ribonuclease H1 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF01693"
FT DOMAIN 535..569
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
SQ SEQUENCE 718 AA; 81532 MW; 6CFEBA0DDDC82DC4 CRC64;
MGKRGPKKNY FVVCRGRTTG ILDGWSQCEA STSGYSGAKF KGYETYDEAQ KAWNEWLQTQ
GFAPTEGAPH DTLENNLTDT SRVVNQTAID NHLEARVGLK RKYEDEDSSL NTNSFVITSA
RPMEAEVNAM STIKTFIDLT ESPEHKISAE KLLDSEGEKT PAFKKAKSGD NTPTLNLDVE
IPDSEFELSD EDIFDNITST CENVPKPVEL TTEQKSVVKM AMGRDNIFLT GAAGSRKTVT
LLQIIENLKN RLQDKNSNTP KVQVAAPTGL AALPLNGRTT YSVAGWKPDS FKMPLDELLD
DNNIKKRTIK ALRKLEVLIL EEISMVESQF LERLNLLLQT VKQTKKPFGG IQVIFLGDFY
QLPPVKPFEH CMICGVEMVG VFDRVCKSNL CKDRSDNTPF RPGDKWAFNA PVWKQLNFRH
VKLEQIHRQK DEKFKDLLNK VRNGVDLTDA EWHDLIRPKV LPPNAFPIRL MAKRFDVDRF
NDSQLELIKS EAKVWEALDD SCQLYRNELD YLRQHEINRK MEEHKIAISE YHRFPKRLTL
KVGAKVVLLH NQEQKGGLVN GSQGTIVGFV NAQKWPTAVI QGDHADWRQI QMNHFIEKNP
WRPVVKFADG KTTAIAPVPS ETLMGTVEDR YLACRTQIPL ILAWALSIHK SQGMTMEYVE
VSRKDIFESG QLYVALSRAT TLEGLTVTGY SREQIAMDED VVDFYQKTQW ENLQPPSE
//
