ID   A0A4Z1KK63_9HELO        Unreviewed;       575 AA.
AC   A0A4Z1KK63;
DT   18-SEP-2019, integrated into UniProtKB/TrEMBL.
DT   18-SEP-2019, sequence version 1.
DT   27-MAR-2024, entry version 14.
DE   RecName: Full=tyrosinase {ECO:0000256|ARBA:ARBA00011906};
DE            EC=1.14.18.1 {ECO:0000256|ARBA:ARBA00011906};
GN   ORFNames=BPOR_0481g00070 {ECO:0000313|EMBL:TGO84652.1};
OS   Botrytis porri.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC   Helotiales; Sclerotiniaceae; Botrytis.
OX   NCBI_TaxID=87229 {ECO:0000313|EMBL:TGO84652.1, ECO:0000313|Proteomes:UP000297280};
RN   [1] {ECO:0000313|EMBL:TGO84652.1, ECO:0000313|Proteomes:UP000297280}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MUCL3349 {ECO:0000313|EMBL:TGO84652.1,
RC   ECO:0000313|Proteomes:UP000297280};
RA   Valero-Jimenez C.A., Tapia P., Veloso J., Silva-Moreno E., Staats M.,
RA   Valdes J.H., Van Kan J.A.L.;
RT   "Comparative genomics of Botrytis spp.";
RL   Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504,
CC         ChEBI:CHEBI:57924; EC=1.14.18.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000426};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924,
CC         ChEBI:CHEBI:58315; EC=1.14.18.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00001038};
CC   -!- COFACTOR:
CC       Name=Cu(2+); Xref=ChEBI:CHEBI:29036;
CC         Evidence={ECO:0000256|ARBA:ARBA00001973};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:TGO84652.1}.
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DR   EMBL; PQXO01000480; TGO84652.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A4Z1KK63; -.
DR   STRING; 87229.A0A4Z1KK63; -.
DR   Proteomes; UP000297280; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004503; F:tyrosinase activity; IEA:UniProtKB-EC.
DR   Gene3D; 2.60.310.20; -; 1.
DR   Gene3D; 1.10.1280.10; Di-copper center containing domain from catechol oxidase; 1.
DR   InterPro; IPR008922; Di-copper_centre_dom_sf.
DR   InterPro; IPR041640; Tyrosinase_C.
DR   InterPro; IPR002227; Tyrosinase_Cu-bd.
DR   PANTHER; PTHR11474:SF32; TYROSINASE; 1.
DR   PANTHER; PTHR11474; TYROSINASE FAMILY MEMBER; 1.
DR   Pfam; PF18132; Tyosinase_C; 1.
DR   Pfam; PF00264; Tyrosinase; 1.
DR   PRINTS; PR00092; TYROSINASE.
DR   SUPFAM; SSF48056; Di-copper centre-containing domain; 1.
DR   PROSITE; PS00497; TYROSINASE_1; 1.
DR   PROSITE; PS00498; TYROSINASE_2; 1.
PE   4: Predicted;
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000297280};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           27..575
FT                   /note="tyrosinase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5021357145"
FT   DOMAIN          137..154
FT                   /note="Tyrosinase copper-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS00497"
FT   DOMAIN          339..350
FT                   /note="Tyrosinase copper-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS00498"
SQ   SEQUENCE   575 AA;  63376 MW;  11171262D99F1125 CRC64;
     MVFFRGASIV SFLTIAGSFL SQTAQAIPRP HDHRPRGYDY GVDIGALLKR DVTPMPTTGV
     SFNGSNSSIP VRQEIRDLQK NADLWELYIL GVSMMQTINQ SDIRSWYQIA GIHGRPYLPY
     DEVQGTPGNE NNGYCTHVSI LFPTWHRPYL ALYEQVLHSV VEFIAAQFPA GAERDRYTAA
     AANFRIPYWD WAVVPPAGES VLPPSVSEPT VTVNGPAGVQ TISNPLFSYH FNPLDPSELP
     DAPFSQFPQT LRYPTSNDAS ATSQNNLVAQ QLDNSAASFR NRLYNLFTNY HDYSTFSNEA
     WITASSPGDF DSIESLHDQI HGLTGSGGHM SYIDYSSFDP LFFLHHTMID RCFAIWQTLN
     PDSYVTPREA SYSTFTMTAG TTQDVSTPLK PFYNADGNDF YTSSDVVSTE SFGYAYPETL
     PGGNTTAQAI KAINLLYGTT APAKTVSRGL IPGLTSKLLG REHQLNSTYD LISLALAPNG
     QYTEWIANIQ VEKFALSRSF FIHVFLGPFN DDPASWSFEP NLVGTHCVFA KLSSSGTVVA
     DPNQLVTGTI PLTSALLDDI SEGHLRSLND VDVEP
//