ID A0A550CJ65_9AGAR Unreviewed; 1409 AA.
AC A0A550CJ65;
DT 16-OCT-2019, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2019, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=5'-3' exoribonuclease 1 {ECO:0000256|PIRNR:PIRNR006743};
DE EC=3.1.13.- {ECO:0000256|PIRNR:PIRNR006743};
GN ORFNames=BD626DRAFT_235395 {ECO:0000313|EMBL:TRM64823.1};
OS Auriculariopsis ampla.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Schizophyllaceae; Auriculariopsis.
OX NCBI_TaxID=97359 {ECO:0000313|EMBL:TRM64823.1, ECO:0000313|Proteomes:UP000320762};
RN [1] {ECO:0000313|EMBL:TRM64823.1, ECO:0000313|Proteomes:UP000320762}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NL-1724 {ECO:0000313|EMBL:TRM64823.1,
RC ECO:0000313|Proteomes:UP000320762};
RX PubMed=31257601;
RA Almasi E., Sahu N., Krizsan K., Balint B., Kovacs G.M., Kiss B.,
RA Cseklye J., Drula E., Henrissat B., Nagy I., Chovatia M., Adam C.,
RA LaButti K., Lipzen A., Riley R., Grigoriev I.V., Nagy L.G.;
RT "Comparative genomics reveals unique wood-decay strategies and fruiting
RT body development in the Schizophyllaceae.";
RL New Phytol. 0:0-0(2019).
CC -!- FUNCTION: Multifunctional protein that exhibits several independent
CC functions at different levels of the cellular processes. 5'-3'
CC exonuclease component of the nonsense-mediated mRNA decay (NMD) which
CC is a highly conserved mRNA degradation pathway, an RNA surveillance
CC system whose role is to identify and rid cells of mRNA with premature
CC termination codons and thus prevents accumulation of potentially
CC harmful truncated proteins. {ECO:0000256|PIRNR:PIRNR006743}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR006743}.
CC -!- SIMILARITY: Belongs to the 5'-3' exonuclease family.
CC {ECO:0000256|PIRNR:PIRNR006743}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TRM64823.1}.
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DR EMBL; VDMD01000006; TRM64823.1; -; Genomic_DNA.
DR STRING; 97359.A0A550CJ65; -.
DR Proteomes; UP000320762; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004534; F:5'-3' RNA exonuclease activity; IEA:UniProt.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR CDD; cd18673; PIN_XRN1-2-like; 1.
DR Gene3D; 1.25.40.1050; -; 1.
DR Gene3D; 2.170.260.40; -; 1.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 2.30.30.750; -; 1.
DR Gene3D; 3.40.50.12390; -; 2.
DR InterPro; IPR027073; 5_3_exoribonuclease.
DR InterPro; IPR016494; 5_3_exoribonuclease_1.
DR InterPro; IPR014722; Rib_uL2_dom2.
DR InterPro; IPR041385; SH3_12.
DR InterPro; IPR040992; XRN1_D1.
DR InterPro; IPR047007; XRN1_D1_sf.
DR InterPro; IPR041106; XRN1_D2_D3.
DR InterPro; IPR041412; Xrn1_helical.
DR InterPro; IPR004859; Xrn1_N.
DR InterPro; IPR047008; XRN1_SH3_sf.
DR PANTHER; PTHR12341:SF41; 5'-3' EXORIBONUCLEASE 1; 1.
DR PANTHER; PTHR12341; 5'->3' EXORIBONUCLEASE; 1.
DR Pfam; PF18129; SH3_12; 1.
DR Pfam; PF18332; XRN1_D1; 1.
DR Pfam; PF18334; XRN1_D2_D3; 1.
DR Pfam; PF17846; XRN_M; 1.
DR Pfam; PF03159; XRN_N; 1.
DR PIRSF; PIRSF006743; Exonuclease_Xnr1; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|PIRNR:PIRNR006743};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|PIRNR:PIRNR006743};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR006743};
KW Nonsense-mediated mRNA decay {ECO:0000256|PIRNR:PIRNR006743};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|PIRNR:PIRNR006743};
KW Reference proteome {ECO:0000313|Proteomes:UP000320762};
KW RNA-binding {ECO:0000256|PIRNR:PIRNR006743}.
FT DOMAIN 1..227
FT /note="Xrn1 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03159"
FT DOMAIN 274..708
FT /note="Xrn1 helical"
FT /evidence="ECO:0000259|Pfam:PF17846"
FT DOMAIN 759..950
FT /note="5'-3' exoribonuclease 1 D1"
FT /evidence="ECO:0000259|Pfam:PF18332"
FT DOMAIN 954..1178
FT /note="Exoribonuclease Xrn1 D2/D3"
FT /evidence="ECO:0000259|Pfam:PF18334"
FT DOMAIN 1198..1264
FT /note="5'-3' exoribonuclease 1 SH3-like"
FT /evidence="ECO:0000259|Pfam:PF18129"
FT REGION 94..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 467..507
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1273..1409
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 100..121
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1302..1328
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1409 AA; 158697 MW; 728A81B1D18A552B CRC64;
MGIPKFFRYI SERYPLTSQL IQENKIPEFD NLYLDFNGII HNCSHPNDAD AHFRMSEEQI
FTGIFSYVDH LFAKIKPKKL LFLAVDGVAP RAKQNQQRSR RFRTAKEVQE VREKAESKGE
KLPEEKAFDS NCITPGTPFM ARLSEQLRYF VNKKITEDAN WHGVEVVLSG HEVPGEGEHK
IMEYIRLSRA QPDYNPNIRH CMYGLDADLV MLSLLSHDPH FCLLREEVKF GPASRKKNSG
GLETINFYLL HLSLMREYLD LEFHDIHSLL PFEYNLERII DDFVLLAVFV GNDFLPNLPD
LHIHENGLER LFDVYKKVLP GLDGYINESG TINTKRLQVV LNEMKEWERE MFEKEYADLN
WYKGKQAKHM REMEMGKKRS KMVLTRPQRD IFDEVQKFVL EGRENGNYKT AKLLMKNDFA
ARERKFVGEL AEELHLCLTW DEYDMMDEEV EGGVNVVVWR FPGALEEPVP EPTKEVGETE
VATNGDSTAP AEAAAAEEDE SEDEESRAAV DRILKKYAKA PVHDDDEGGG FDARHDRAME
EKMDEWKRNY YRGKLEIDYD DPQELGDLIF RYVEGLQWVM HYYYSGVASW GWFYNYHYAP
RMSDLVGVDK MTFDFDLGRP FRPFEQLMGV LPAASKDHIP SAYQDLMYDE HSPIIDFYPT
EFESDLNGKK QEWEAVVKIP FIDEKRLLSA MGKREHRLTE EEKQRNSFST STKFSYKADG
DPFFYPSSLP GFFPAIAKCR CNMEPFDLPT LNGLHLIDGL CEGVFLGADA LAGFPSLKTL
PHTATLGHHG VNVHGSESRN KSMVVHVQNE REGIKVEDVA QAMIGQRIYT GWPFLQEGKV
VAVSDALFKY EMMSVAPGTK ARVISTPHSQ PGLGHWKGKA ERIQDVYSKR CGVITGDVEV
LLHVRPLKGL MRLDTGAFVK DYEGPDKEIE AALQMTVSNV VSEDPRYTER EAPPLADEFP
DGTKIFFLGE HAYGVAAQVS STSDNTLSVI LAFFPSEKSE NDQFKAIVNN RKSARYLPSF
QVAQMLDMSG RALSRITSSF MVVTSDGSKT NLGLSLKFEA KGMKVVDYSR KSGRNWEFSE
KAIDLLREYK QTYPDVFRAL DQDGDAMARA SDVLPGQNSD ARVKEVKAWL KEKGVRDFEP
VTLFCDQLGK DMVKEIEQLA DGFNSSKSTS GFKKAIVKGI PRQAVLKPAH AQYRLQNQRF
ALGDRVTMIQ DSGAVPLSVK GTVIGLNAKS MDIVWDVAFM AGTTLGDRCS LYRGSTVEFN
TCLNLSNPQF VASTNPKTPA APARNGPAFK PQIGPRPVIQ PGVGQQSASG FRPAPQQQGG
NPVQIMSNPN RGGRGGRGGP PPRPYNETAQ AADMAPAAGR GGPFPRGGRG FAPPFRGRGG
FAPGFDRGRG APRGMNRGRR RGFAPPLQS
//
