ID A0A5A8C1N6_CAFRO Unreviewed; 960 AA.
AC A0A5A8C1N6;
DT 13-NOV-2019, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2019, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=tRNA (guanine(26)-N(2))-dimethyltransferase {ECO:0000256|ARBA:ARBA00039099};
DE EC=2.1.1.216 {ECO:0000256|ARBA:ARBA00039099};
GN ORFNames=FNF29_07916 {ECO:0000313|EMBL:KAA0146674.1};
OS Cafeteria roenbergensis (Marine flagellate).
OC Eukaryota; Sar; Stramenopiles; Bigyra; Opalozoa; Bicosoecida;
OC Cafeteriaceae; Cafeteria.
OX NCBI_TaxID=33653 {ECO:0000313|EMBL:KAA0146674.1, ECO:0000313|Proteomes:UP000323011};
RN [1] {ECO:0000313|EMBL:KAA0146674.1, ECO:0000313|Proteomes:UP000323011}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BVI {ECO:0000313|EMBL:KAA0146674.1,
RC ECO:0000313|Proteomes:UP000323011};
RA Fischer M.G., Hackl T., Roman M.;
RT "Genomes of Cafeteria roenbergensis.";
RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Trm1 family. {ECO:0000256|PROSITE-ProRule:PRU00958}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAA0146674.1}.
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DR EMBL; VLTN01000081; KAA0146674.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5A8C1N6; -.
DR Proteomes; UP000323011; Unassembled WGS sequence.
DR GO; GO:0160102; F:tRNA (guanine(10)-N2)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR Gene3D; 3.30.56.70; N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002905; Trm1.
DR InterPro; IPR042296; tRNA_met_Trm1_C.
DR PANTHER; PTHR10631; N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR10631:SF3; TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE; 1.
DR Pfam; PF02005; TRM; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 2.
DR PROSITE; PS51626; SAM_MT_TRM1; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; Reference proteome {ECO:0000313|Proteomes:UP000323011};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00958};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU00958};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; tRNA processing {ECO:0000256|PROSITE-ProRule:PRU00958};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|PROSITE-
KW ProRule:PRU00958}.
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 366..452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 521..540
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 559..579
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 595..621
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 669..738
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 842..869
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 918..960
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 401..417
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 689..706
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 715..730
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 933..960
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 960 AA; 98193 MW; 937166618C963600 CRC64;
MSSSGPASSM SAAEGGHVPP GLTPPEGFKT IVEGSATALY PDGRAVFYNP AQVYNRDLST
AALRVLAKER AIHWSSPDAA AHEVNRRSKR DSRDDGLGGW VPPAIKAILF APPAEELARL
DEYHAKSAEE RSELASAAAA TASGPAPGAL GASLGVEVVE ALAATGLRSV RYASEVPGLG
AVVVSDLDGA AVRACKHNVA FNGLDPAVVR PCQADAVSLM QAMRAPEWRV DWLDLDPYGT
PSPFIESAVS AVHHGGILAM TATDMIVLAG SMRSAAHGKY GSMAVRSAAC HELALRALIF
TVATAAARAK RTVEPLLSVN ANFYCRIFFR VRNNPAGAQN LSLMTGSLAQ CPQCGTRQWQ
PVGRVSTQAA PGKYGSRKGK GGRPAFRGRR PRITRGGAAA ACRGTSSSSS SAASGSKPGA
EAGNGADDAE TGDAAEATPS AGPDAEERAA QAERVRAAVL GLARGDDDAV RGAKAMPARV
AVSPLCPSCG GNMHVAGPYW TGPIHSPPFV RAVREEVKAL ADSDSGKRGG GFPVAPGEGA
VLPGRRLPME AVLSGQAGAG AHCAPAPSSS SSGGSTAAAG GAAAAAGSMV DGEPAVSPLG
VSTGLGKKEA HDAGSNTDET VAASRRRLLS MLRLMSREVG GEELERMYAA LDGDGQGVAG
SDAWNAALPR SGARPIGASG PAADGDQGEG SDGEGEGSDG EGEGSDGEGE GQSAQGAAPS
ATSAAASATS GRGPSDAALA VSAEQRTLNG LLYWNANTLG MATKMPLPPR RGLAACIINE
GFRVSDTHCD ETGFKTDAPI ALLWDALRTF GWSAEGMYPE QGLAKAREDL VTVRAKMAEA
RAAQESGSGA GAEGGKRFPN KQRKRQAQLL GGQALASATL LASPITFTGL TFEPRTDAAR
AALRKFLGYE ASADGETKVR KHFANPRGEW GPQQRAKGDK RPTDATDAES EPKRARPSEE
//
