ID A0A5B0QJG9_PUCGR Unreviewed; 751 AA.
AC A0A5B0QJG9;
DT 13-NOV-2019, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2019, sequence version 1.
DT 27-MAR-2024, entry version 12.
DE RecName: Full=5'-3' exoribonuclease {ECO:0000256|PIRNR:PIRNR037239};
DE EC=3.1.13.- {ECO:0000256|PIRNR:PIRNR037239};
GN ORFNames=PGT21_027595 {ECO:0000313|EMBL:KAA1113306.1};
OS Puccinia graminis f. sp. tritici.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia.
OX NCBI_TaxID=56615 {ECO:0000313|EMBL:KAA1113306.1, ECO:0000313|Proteomes:UP000324748};
RN [1] {ECO:0000313|EMBL:KAA1113306.1, ECO:0000313|Proteomes:UP000324748}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=21-0 {ECO:0000313|EMBL:KAA1113306.1};
RA Li F., Upadhyaya N.M., Sperschneider J., Matny O., Nguyen-Phuc H., Mago R.,
RA Raley C., Miller M.E., Silverstein K.A.T., Henningsen E., Hirsch C.D.,
RA Visser B., Pretorius Z.A., Steffenson B.J., Schwessinger B., Dodds P.N.,
RA Figueroa M.;
RT "Emergence of the Ug99 lineage of the wheat stem rust pathogen through
RT somatic hybridization.";
RL Submitted (MAY-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Possesses 5'->3' exoribonuclease activity. May promote
CC termination of transcription by RNA polymerase II.
CC {ECO:0000256|PIRNR:PIRNR037239}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the 5'-3' exonuclease family. XRN2/RAT1
CC subfamily. {ECO:0000256|ARBA:ARBA00006994,
CC ECO:0000256|PIRNR:PIRNR037239}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAA1113306.1}.
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DR EMBL; VSWC01000015; KAA1113306.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5B0QJG9; -.
DR Proteomes; UP000324748; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0004534; F:5'-3' RNA exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006353; P:DNA-templated transcription termination; IEA:UniProtKB-KW.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-UniRule.
DR CDD; cd09897; H3TH_FEN1-XPG-like; 1.
DR CDD; cd18673; PIN_XRN1-2-like; 1.
DR Gene3D; 1.25.40.1050; -; 1.
DR Gene3D; 3.40.50.12390; -; 2.
DR InterPro; IPR027073; 5_3_exoribonuclease.
DR InterPro; IPR041412; Xrn1_helical.
DR InterPro; IPR004859; Xrn1_N.
DR InterPro; IPR017151; Xrn2/3/4.
DR PANTHER; PTHR12341:SF41; 5'-3' EXORIBONUCLEASE 1; 1.
DR PANTHER; PTHR12341; 5'->3' EXORIBONUCLEASE; 1.
DR Pfam; PF17846; XRN_M; 2.
DR Pfam; PF03159; XRN_N; 1.
DR PIRSF; PIRSF037239; Exonuclease_Xrn2; 2.
PE 3: Inferred from homology;
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|PIRNR:PIRNR037239};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR037239};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664,
KW ECO:0000256|PIRNR:PIRNR037239};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|PIRNR:PIRNR037239};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000324748};
KW rRNA processing {ECO:0000256|ARBA:ARBA00022552};
KW Transcription {ECO:0000256|ARBA:ARBA00022472};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00022472};
KW Transcription termination {ECO:0000256|ARBA:ARBA00022472}.
FT DOMAIN 1..227
FT /note="Xrn1 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03159"
FT DOMAIN 271..356
FT /note="Xrn1 helical"
FT /evidence="ECO:0000259|Pfam:PF17846"
FT DOMAIN 508..682
FT /note="Xrn1 helical"
FT /evidence="ECO:0000259|Pfam:PF17846"
SQ SEQUENCE 751 AA; 87130 MW; 1A1DF61652274249 CRC64;
MGIPKFFRWM TQKYPVVLHQ VIQGDCIPEI DNLYLDMNGI IHNCSHPSTP TADFQITEPE
IFSGIFAALE HLFTLAKPKK LFFMAVDGVA PRAKMNEQRS RRFKAAQANQ TMLQRNAFRR
FQTVSNSFDS NCITPGTPFM ARLHEQLKYF INKKVTEDPA WQAVQVIFSG HDVPGEGEHK
IMEYIRRAKT QQDYSPHIRH CIYGLDADLI MLALLSHEPH FCLLREENRA PLKRKPTINL
KDQQFHLFQI SLFRDYLNWE FSSLQLIKNF KYDIRRIIDD FILLCIFVGN DFLPHLPNLH
IDDGAIGVLF KIYKKILPQA GGYLNDSGTL HTHRLQLLLD ELVSHEYQHF ETSTGSNQKC
VGIPGTNFPL NDTQRALVDR VESFFRTHTY SPNKWTAKLV LPGCLDRQDR NVLRDLADKL
DLWISFDNDP DTHAPLISLG FFQDDLLLTD PVVDAVHGIL RDLQGNGTSQ PETPTQIHTL
DSCTEEILRK IKRTPGNWGM YGRKPPKQSD DDDFHAYLKQ WKSKYYKNKM ELQYNDSVQL
HKLTHAYVEG LQWILQYYYT GVASWSWAYP YHYAPMVSDL NKVASYKFSF ELGSPFKPFE
QLMGVLPGLS STHVPSAFQD LMTKPDSPIL DLYPTSFETD LNGKRHEWQA IAKIPFIDSR
RLLEAMKAHE YKLKDEEIER NRFGPTWQFR YTPLSPTYYR SLLPSYFPDL YPCLSEMTVY
HLPTVPQKIL GRLKETDEIA KNIEKLMALC L
//
