ID   A0A5C6UKF1_9SPHN        Unreviewed;       978 AA.
AC   A0A5C6UKF1;
DT   13-NOV-2019, integrated into UniProtKB/TrEMBL.
DT   13-NOV-2019, sequence version 1.
DT   27-MAR-2024, entry version 13.
DE   SubName: Full=Insulinase family protein {ECO:0000313|EMBL:TXC73553.1};
GN   ORFNames=FSZ31_02050 {ECO:0000313|EMBL:TXC73553.1};
OS   Sphingorhabdus soli.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingorhabdus.
OX   NCBI_TaxID=2601267 {ECO:0000313|EMBL:TXC73553.1, ECO:0000313|Proteomes:UP000321129};
RN   [1] {ECO:0000313|EMBL:TXC73553.1, ECO:0000313|Proteomes:UP000321129}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=D-2Q-5-6 {ECO:0000313|EMBL:TXC73553.1,
RC   ECO:0000313|Proteomes:UP000321129};
RA   Liu Y.;
RT   "Sphingorhabdus soil sp. nov., isolated from arctic soil.";
RL   Submitted (AUG-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947};
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:TXC73553.1}.
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DR   EMBL; VOPY01000001; TXC73553.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A5C6UKF1; -.
DR   OrthoDB; 9811314at2; -.
DR   Proteomes; UP000321129; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   PANTHER; PTHR43690:SF21; PROCESSING PROTEASE; 1.
DR   Pfam; PF00675; Peptidase_M16; 2.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000321129};
KW   Signal {ECO:0000256|SAM:SignalP}; Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           33..978
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5022882769"
FT   DOMAIN          75..202
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          232..407
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          561..677
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          711..889
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   978 AA;  104801 MW;  7A55445606D2132D CRC64;
     MNAMLTRSSR LARILLAASP AALIAGCATM PAADMAATPA APAAPAAFEP APVSTLVDAV
     NIPYEKFTLS NGLTVLVHED RKAPVVAVSV WYNVGSKDEP KGKTGFAHLF EHLMFNGSEN
     SPGDFFEPLQ QVGATDFNGT TNVDRTNYFE TVPTGALERA LMLESDRMGH LLGAVTQEKL
     DNQRGVVQNE KRQGDNQPYG LLRYEVQENL FPAGHPYHHS TIGSMADLNA ASLDDVKNWF
     RANYGPNNSV LVLAGDIDTA TARTLVTKWF GDIPRGPTVT HPDAPVTPLA APIDKTVTDQ
     IATTRLYRMW PIPGFTSTES IPLQLAASVL GGLSSSRLDN ALVRKDPVAV SVSTSTWDFM
     EGGAFIVQAD VKPGEDAAVV AQKLDAQIAD YLATGPSADE LQRAKTSMVS GIIGGLESVG
     GFGGKAVALA EGELYSDNPD YYKVQLDQMA AATPEQVTET MRKWLTKPRF TLVMKPGERT
     EGGENRGGAA IGNYLDQAEV APAPLDYYCD PKTCDAAALT LSYDGDRSKL PSVGQLQELD
     FPDIERARLA NGMEIYFARR TTVPKVTVRV AFDAGYAADP KSALGTQSLM LGMMDEGTRN
     LDSIALAEAQ ERLGANINGF ADADWTSFQL SALAPNLAPS LALLADYVRN PAFDAKVMER
     VRAQQLNRLN AELKNPQGIA ARALAPLLYG PDHPYGIPPS GLGNASAVAA LTPADLAAFH
     DAWLRPDDAR IFVVGDTTLA EVTALLNRSF GNWTATGAAH PVKNFAVATP EPQPRIVLID
     RPNSPQSIIT AGRVLDLKGA DDLVPLMAAN EVFGGSFLSR INMNLRETKG WSYGVRSGVQ
     GVVDRTAFTI SAPVQADRTG DSIRELRKEL TGYTSDKGIT ADELERTING NVRELPGQFE
     TSGAVLGGMV NIVKYGRPDN FYETLAARYR TMTAAGLDAV ARKEFLDNEL VFVVVGDAKV
     VRPQLDGLGL TVEEATAK
//