ID A0A5C7EXH5_9PROT Unreviewed; 903 AA.
AC A0A5C7EXH5;
DT 13-NOV-2019, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2019, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Molybdopterin-dependent oxidoreductase {ECO:0000313|EMBL:TXF13038.1};
GN ORFNames=FR698_02885 {ECO:0000313|EMBL:TXF13038.1};
OS Pelomicrobium methylotrophicum.
OC Bacteria; Pseudomonadota; Hydrogenophilia;
OC Hydrogenophilalia incertae sedis; Pelomicrobium.
OX NCBI_TaxID=2602750 {ECO:0000313|EMBL:TXF13038.1, ECO:0000313|Proteomes:UP000321201};
RN [1] {ECO:0000313|EMBL:TXF13038.1, ECO:0000313|Proteomes:UP000321201}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SM250 {ECO:0000313|EMBL:TXF13038.1,
RC ECO:0000313|Proteomes:UP000321201};
RA Slobodkina G.B., Merkel A.Y., Slobodkin A.I.;
RT "Pelomicrobium methylotrophicum gen. nov., sp. nov. a moderately
RT thermophilic, facultatively anaerobic, lithoautotrophic and methylotrophic
RT bacterium isolated from a terrestrial mud volcano.";
RL Submitted (AUG-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Nitrogen metabolism. {ECO:0000256|ARBA:ARBA00004909}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. NasA/NapA/NarB subfamily.
CC {ECO:0000256|ARBA:ARBA00008747}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TXF13038.1}.
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DR EMBL; VPFL01000003; TXF13038.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5C7EXH5; -.
DR InParanoid; A0A5C7EXH5; -.
DR OrthoDB; 9803192at2; -.
DR Proteomes; UP000321201; Unassembled WGS sequence.
DR GO; GO:1990204; C:oxidoreductase complex; IEA:UniProt.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0045333; P:cellular respiration; IEA:UniProt.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd02791; MopB_CT_Nitrate-R-NapA-like; 1.
DR CDD; cd02754; MopB_Nitrate-R-NapA-like; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.40.50.740; -; 1.
DR Gene3D; 2.20.25.90; ADC-like domains; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.40.228.10; Dimethylsulfoxide Reductase, domain 2; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR041957; CT_Nitrate-R-NapA-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR PANTHER; PTHR43105:SF9; NITRATE REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G15190)-RELATED; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF04879; Molybdop_Fe4S4; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000321201}.
FT DOMAIN 1..57
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 903 AA; 98168 MW; 2123C0D857029B66 CRC64;
MPKVKSTCCY CGVGCGVVIE TDDGRITAIA GDADHPANFG RLCSKGATLH LTAGTAGRAL
YPELRRSRSE PRRRVEWDQA LDHLAARVAR VILEHGPDAV GFYISGQLLT EDYYVFNKLV
KGLIGTNNID TNSRLCMSSA VAGYKATLGA DSVPTCYADI EHAHCLFIAG SNTAYAHPVL
FRRIEEARRA NPAVKMIVVD PRRTDTAASA DLHLPILPGT DVALFNGMLH VMVWEGRLDR
AFIEAHTTGF DALKEAVQEY TPKTVAGLCG VPAEDIVQAA RWFADSPATL SLYCQGLNQS
SHGTDKNAAL INLHLATGQI GRPGAGPFSL TGQPNAMGGR EVGGMANLLS GHRDLANPAH
RAEVARLWGV AEVPATPGKT AVEMFEAARA GEIKVLWIAC TNPAQSMPDQ ALVRAALETA
ELVVVQEAYR DTETAAYADV LLPAATWGEK EGTVTNSERR ISRVRAAVPP PGEARPDWVI
AVDFARRLEW RLRFGNRGEH FSTLFPYRAP EEVFNEHRET TRGRDLDITG LSYALLDALG
PQQWPFPAGA ARGAERLYAD GVFPTADGRA RFVATRYRTP AESPDARYPF RLNTGRLRDQ
WHGMSRTGRV ARLFSHEEEP LLAMNPEDMA RRGLRDGDLA RVASRRGAIV VRVKASEEQR
PGQVFLPMHW GSRFMAGHGT NALTVSVFDP TSKQPELKHA AVRVEKAALP WQLVAMAVGD
VQARLSSLQP LLERFDYASC GLFGHEEEGV ILRAAAASAV DTELVAELDR IFGLEPEAGV
MSYMDAHRGV AKRVRIANGR LVAARLTGET AAQAWLKEWM TGGMPVDELR RFLLAPVPAA
PSGFSPRGRV VCNCLNVSER EIVAAIDAGA NLQTLQQTLQ CGTQCGSCVP ELKRLLIARR
QAA
//
