ID A0A5F5Y5B9_FELCA Unreviewed; 1089 AA.
AC A0A5F5Y5B9;
DT 11-DEC-2019, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2019, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=MCF.2 cell line derived transforming sequence like {ECO:0000313|Ensembl:ENSFCAP00000061571.1};
GN Name=MCF2L {ECO:0000313|Ensembl:ENSFCAP00000061571.1,
GN ECO:0000313|VGNC:VGNC:63411};
OS Felis catus (Cat) (Felis silvestris catus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Feliformia; Felidae; Felinae; Felis.
OX NCBI_TaxID=9685 {ECO:0000313|Ensembl:ENSFCAP00000061571.1, ECO:0000313|Proteomes:UP000011712};
RN [1] {ECO:0000313|Ensembl:ENSFCAP00000061571.1, ECO:0000313|Proteomes:UP000011712}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Abyssinian {ECO:0000313|Ensembl:ENSFCAP00000061571.1,
RC ECO:0000313|Proteomes:UP000011712};
RX PubMed=17975172; DOI=10.1101/gr.6380007;
RA Pontius J.U., Mullikin J.C., Smith D.R., Lindblad-Toh K., Gnerre S.,
RA Clamp M., Chang J., Stephens R., Neelam B., Volfovsky N., Schaffer A.A.,
RA Agarwala R., Narfstrom K., Murphy W.J., Giger U., Roca A.L., Antunes A.,
RA Menotti-Raymond M., Yuhki N., Pecon-Slattery J., Johnson W.E., Bourque G.,
RA Tesler G., O'Brien S.J.;
RT "Initial sequence and comparative analysis of the cat genome.";
RL Genome Res. 17:1675-1689(2007).
RN [2] {ECO:0000313|Ensembl:ENSFCAP00000061571.1, ECO:0000313|Proteomes:UP000011712}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Abyssinian {ECO:0000313|Ensembl:ENSFCAP00000061571.1,
RC ECO:0000313|Proteomes:UP000011712};
RA Hillier L.W., Warren W., Obrien S., Wilson R.K.;
RT "Sequence assembly of the Felis catus genome version 6.2.";
RL Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Ensembl:ENSFCAP00000061571.1}
RP IDENTIFICATION.
RC STRAIN=breed Abyssinian {ECO:0000313|Ensembl:ENSFCAP00000061571.1};
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; AANG04001781; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; A0A5F5Y5B9; -.
DR Ensembl; ENSFCAT00000078434.1; ENSFCAP00000061571.1; ENSFCAG00000014242.6.
DR VGNC; VGNC:63411; MCF2L.
DR GeneTree; ENSGT00940000157874; -.
DR Proteomes; UP000011712; Chromosome A1.
DR Bgee; ENSFCAG00000014242; Expressed in prefrontal cortex and 9 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR CDD; cd01227; PH_Dbs; 1.
DR CDD; cd00160; RhoGEF; 1.
DR CDD; cd00170; SEC14; 1.
DR CDD; cd11857; SH3_DBS; 1.
DR CDD; cd00176; SPEC; 1.
DR Gene3D; 1.20.58.60; -; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 2.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR Gene3D; 2.30.30.40; SH3 Domains; 1.
DR InterPro; IPR001251; CRAL-TRIO_dom.
DR InterPro; IPR036865; CRAL-TRIO_dom_sf.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR035534; DBS_PH.
DR InterPro; IPR035532; DBS_SH3.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR001331; GDS_CDC24_CS.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR InterPro; IPR018159; Spectrin/alpha-actinin.
DR InterPro; IPR002017; Spectrin_repeat.
DR PANTHER; PTHR22826:SF115; GUANINE NUCLEOTIDE EXCHANGE FACTOR DBS; 1.
DR PANTHER; PTHR22826; RHO GUANINE EXCHANGE FACTOR-RELATED; 1.
DR Pfam; PF13716; CRAL_TRIO_2; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00621; RhoGEF; 2.
DR Pfam; PF00435; Spectrin; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SMART; SM00516; SEC14; 1.
DR SMART; SM00150; SPEC; 1.
DR SUPFAM; SSF52087; CRAL/TRIO domain; 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR SUPFAM; SSF50044; SH3-domain; 1.
DR SUPFAM; SSF46966; Spectrin repeat; 1.
DR PROSITE; PS50191; CRAL_TRIO; 1.
DR PROSITE; PS00741; DH_1; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50002; SH3; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000011712};
KW SH3 domain {ECO:0000256|ARBA:ARBA00022443, ECO:0000256|PROSITE-
KW ProRule:PRU00192}.
FT DOMAIN 44..216
FT /note="CRAL-TRIO"
FT /evidence="ECO:0000259|PROSITE:PS50191"
FT DOMAIN 623..772
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
FT DOMAIN 790..906
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 1016..1076
FT /note="SH3"
FT /evidence="ECO:0000259|PROSITE:PS50002"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 547..617
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 916..993
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 592..617
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1089 AA; 122187 MW; 1800562997C9F74D CRC64;
MAAKGASRGA PGGPQRRLWL LPRRRRGTSR RPQPVEAGRG GTTRDAGADE IMHQDITPLC
AADIQDQLKK RFAYLSGGRG QDGSPVITFP DYPAFSDVPD KEFQNVMTYL TSIPSLQDAG
IGFILVIDRR QDKWTSVKAS VLRIAASFPA NLQLVLVLRP AGFFQRTLSD LAFKFNRDDF
KMKVPVIMLS SVPELHGYID KSQLTEDLGG TLDYCHTRWL CHRTAIESFA LMVKQTAQML
QSFGTELAET ELPNDVQSTS SVLCAHTEKK DKAKEDMRAA LDEGQSLLQS VREPLAKGGG
RSLSQDQLDN QTTVQRLLAQ LNETEAAFDE FWAKHQQKLQ QCLQLRHFEQ DFREVKASLD
ALAQKIATFT DVGNSLAHAG HLLKDLTSFE EKSSVSVERA LALALEGEKL IDSRHYAVDS
IRPKCHELQH LCNQFAAEVE RRRELLSKSL ELHSLLEASM KWCDEGIYLL ASQPVDKCQS
QEGAEAALQE IEKFLETGAE NKIQELDRIY QDYESVLTTD LMEHVQKVYQ KQEGTEEMFH
RRQASLKKLA AKQTRPVQPV APRPEALTKS PCPSPGSRRG SENSPEGSML RRGPYRRAKS
EVTESRQGRG SSTGDEEESL AILRRHVMNE LLDTERVYVE ELLCVLEGYA AELDNPLMAH
LVSPGLQNKK DILFGNMEEI YHFHNRIFLR ELENYTDCPE LVGRCFLERE CQRKLDHKLS
LDSYLLKPVQ RITKYQLLLK EMLKYSKSCE GAEDLQEALS SILGILKAVN DSMHLIAITG
YDGNLSDLGR LLMQGSFSVW TDHKKGHTKV KDLARFKPMQ RHLFLHEKAV LFCKKREENG
EGYEKAPSYS YKQSLNMAAV GITENVKGDV RKFEIWYNAR EEVYIIQAPT VEIKAAWVSE
IRKVLTSQLQ ACREASQHRA LEQSQSLPLP TPPSTSPSKG NTRNIKKLEE RKTDPLSLEG
YVSSTPLPKP PEKGRGWSKT SHPSEAPEDD GWSSAEELIN SSDAEEEGRV GPRKLVPGKY
TVVLGDEKES PEALAVRSGD VVEVVQEGDE GLWYVRNLTS SKESWVPASS LSTLLGTSGS
AQCLSSSVW
//
