ID A0A5J4NIC1_9TREM Unreviewed; 768 AA.
AC A0A5J4NIC1;
DT 11-DEC-2019, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2019, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=tRNA (cytosine(34)-C(5))-methyltransferase {ECO:0000256|ARBA:ARBA00012629};
DE EC=2.1.1.203 {ECO:0000256|ARBA:ARBA00012629};
GN ORFNames=DEA37_0007489 {ECO:0000313|EMBL:KAA3675315.1};
OS Paragonimus westermani.
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda;
OC Digenea; Plagiorchiida; Troglotremata; Troglotrematidae; Paragonimus.
OX NCBI_TaxID=34504 {ECO:0000313|EMBL:KAA3675315.1, ECO:0000313|Proteomes:UP000324629};
RN [1] {ECO:0000313|EMBL:KAA3675315.1, ECO:0000313|Proteomes:UP000324629}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IND2009 {ECO:0000313|EMBL:KAA3675315.1,
RC ECO:0000313|Proteomes:UP000324629};
RX PubMed=30520948;
RA Oey H., Zakrzewski M., Narain K., Devi K.R., Agatsuma T., Nawaratna S.,
RA Gobert G.N., Jones M.K., Ragan M.A., McManus D.P., Krause L.;
RT "Whole-genome sequence of the oriental lung fluke Paragonimus westermani.";
RL Gigascience 8:0-5232231(2019).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|ARBA:ARBA00007494,
CC ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAA3675315.1}.
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DR EMBL; QNGE01002590; KAA3675315.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5J4NIC1; -.
DR Proteomes; UP000324629; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0016428; F:tRNA (cytidine-5-)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProt.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR023270; RCMT_NCL1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22808; NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR22808:SF1; RNA CYTOSINE C(5)-METHYLTRANSFERASE NSUN2; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR PRINTS; PR02011; RCMTNCL1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS01153; NOL1_NOP2_SUN; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000324629};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; tRNA processing {ECO:0000256|ARBA:ARBA00022694};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
FT DOMAIN 42..442
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT REGION 733..768
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 325
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 167..173
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 206
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 233
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 266
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 768 AA; 88020 MW; 34130814F684BCAE CRC64;
MVRKRRPRVG HHQFTPETRQ NFQFEQYYKG QGLIVDSEWP AFMHALQQEL PVTFRVTGFR
EQNRELLRLI KHRYRNEIIK LQFPATDSGE VQQQHISFDS LSWYPNELAW QLDASRFVVR
KTPELKVLHH FLVSETESGN ISRQEAVSML PPLLLDIRSY HSVLDLCAAP GSKSAQLVEL
LHADAETKRT ADWSYTEPTG LVVANDVDQK RCYMMVHQVK RLQSPCTVIT QEDATCFPRL
YVPDPSTLKT DKVEMTQFLF DRVLADVPCS GDGTLRKNPD LWLRWSPNLG IAEHYLQLRI
LRRGLELLRD TTSDGDHGRL VYSTCSFNPL ENEAVIASML HACQGAVRLV GPDRLACVTR
RGPDASRGRF QTRPGLTSWR VMNKKGCWFD KFEDVPQSQQ CNLRPSLFPP PNITELGLDR
CFRVFPQDQN TGGFFLAVLE KVAPLPWMKP TGPRSYTFVG PIVSGICDQE EAEDASDLIE
TDFPPEPNKQ TCENECSSAI ESKKPRVFNE NPFTYIDPSD DKDWANIRDY YGIMETSDHD
AVDPKNGVIR NFCPANLLYR STGDELRRRQ FYYTNKLAKQ IIQSNLCRGI KIVNCGVRMF
CIMEDKQFTG YRLLQDGVDL ADAFLPETGG RRIHLTWMQY SDLKLLLEEE MPLISRMTMT
TQSQWNRLTP GPVLIDYSPS NTEVETEQNL PQCRLVFAGW RGIQSLRHYI GRHERLHLMR
LANLDPKPAI MGHTVLDQKR PRDGASPIST EPDAYESDRD PDVISSAS
//
