ID A0A5J9WH24_9POAL Unreviewed; 708 AA.
AC A0A5J9WH24;
DT 11-DEC-2019, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2019, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=tRNA (guanine(26)-N(2))-dimethyltransferase {ECO:0000256|ARBA:ARBA00039099};
DE EC=2.1.1.216 {ECO:0000256|ARBA:ARBA00039099};
DE Flags: Fragment;
GN ORFNames=EJB05_07195 {ECO:0000313|EMBL:TVU47589.1};
OS Eragrostis curvula (weeping love grass).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Chloridoideae; Eragrostideae; Eragrostidinae; Eragrostis.
OX NCBI_TaxID=38414 {ECO:0000313|EMBL:TVU47589.1, ECO:0000313|Proteomes:UP000324897};
RN [1] {ECO:0000313|EMBL:TVU47589.1, ECO:0000313|Proteomes:UP000324897}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Victoria {ECO:0000313|Proteomes:UP000324897};
RC TISSUE=Leaf {ECO:0000313|EMBL:TVU47589.1};
RX PubMed=31308395; DOI=.1038/s41598-019-46610-0;
RA Carballo J., Santos B.A.C.M., Zappacosta D., Garbus I., Selva J.P.,
RA Gallo C.A., Diaz A., Albertini E., Caccamo M., Echenique V.;
RT "A high-quality genome of Eragrostis curvula grass provides insights into
RT Poaceae evolution and supports new strategies to enhance forage quality.";
RL Sci. Rep. 9:10250-10250(2019).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Trm1 family. {ECO:0000256|PROSITE-ProRule:PRU00958}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TVU47589.1}.
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DR EMBL; RWGY01000004; TVU47589.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5J9WH24; -.
DR Proteomes; UP000324897; Chromosome 5.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0160102; F:tRNA (guanine(10)-N2)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006275; P:regulation of DNA replication; IEA:InterPro.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.70.10.10; -; 1.
DR Gene3D; 3.30.56.70; N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR046938; DNA_clamp_sf.
DR InterPro; IPR022649; Pr_cel_nuc_antig_C.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002905; Trm1.
DR InterPro; IPR042296; tRNA_met_Trm1_C.
DR NCBIfam; TIGR00308; TRM1; 1.
DR PANTHER; PTHR10631; N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR10631:SF3; TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE; 1.
DR Pfam; PF02747; PCNA_C; 1.
DR Pfam; PF02005; TRM; 1.
DR SUPFAM; SSF55979; DNA clamp; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51626; SAM_MT_TRM1; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; Reference proteome {ECO:0000313|Proteomes:UP000324897};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00958};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU00958};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU00958}; tRNA processing {ECO:0000256|PROSITE-ProRule:PRU00958};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|PROSITE-
KW ProRule:PRU00958}.
FT DOMAIN 629..708
FT /note="Proliferating cell nuclear antigen PCNA C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02747"
FT REGION 56..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 101..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 555..601
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..77
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 584..599
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 708
FT /evidence="ECO:0000313|EMBL:TVU47589.1"
SQ SEQUENCE 708 AA; 78559 MW; B0E3C38AD2D1D20D CRC64;
MGEVEEKLKD YEIKREGEAE ILMLKSNAVF FNPVQVHNRD MSIAVLRTFV NKRKEEHEAQ
LNKRNKSHQK DKQSETSVPN GEDVSGSQND EMDVRENELN QAVDGTNDPS KEAPKTPSWK
VTRELKPPIV LEALAASGLR SLRYAREVEG LGKVVATDND KASVEACKRN IKFNGASAVS
KVEAHLADAR VYMLTHPKEF DVVDLDPYGS PSIFLDSAVQ AVADGGLLMC TATDMAVLCG
TNGEVCYSKY GSYPVKGKYC HEMALRILLA SIESHANRYK RYIVPVLSVF MDFYVRVFVR
VFTSASEIKN TPLKLSYVYQ CVGCDSFHLQ CLGRTISKNN SVKHAPGIGP VVPQECSDCG
KKFNVGGPIW SAPIHDQDWV VSTLTDVKSM KDRYPAYDKI TSVLTTVSEE LHDIPLFFSL
HNIAGTVKCT SPSLVMFRSA VINAGYRISS THVNPLGVKS DAPWDVIWDI MRCWVKNHPI
KEQPRDSPGT AILSKPPTIE ANFSRAVAAL SKAQAKKVKR FLPNPERHWG PKVRAGRKIT
SKHVSLLGPD AINGVLNGDI SHEDGNGPAP DESAPEPEEI KDDGENEPTT KRQKISDDEP
AKANNTTVYD LWLVDANIKR FQIPDWQVLD SKYQAFVQMP SAEFMRVCKC LSNIGDDGDI
SVTEKGLKFF AKGKSGRVNI KYMQPEATAT VVTMRAPVSM TLDLKYMN
//
