ID   A0A5M3VUZ9_9ACTN        Unreviewed;       673 AA.
AC   A0A5M3VUZ9;
DT   26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT   26-FEB-2020, sequence version 1.
DT   24-JAN-2024, entry version 12.
DE   SubName: Full=Endonuclease {ECO:0000313|EMBL:GES00396.1};
GN   ORFNames=Acor_24600 {ECO:0000313|EMBL:GES00396.1};
OS   Acrocarpospora corrugata.
OC   Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC   Streptosporangiaceae; Acrocarpospora.
OX   NCBI_TaxID=35763 {ECO:0000313|EMBL:GES00396.1, ECO:0000313|Proteomes:UP000334990};
RN   [1] {ECO:0000313|EMBL:GES00396.1, ECO:0000313|Proteomes:UP000334990}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 13972 {ECO:0000313|EMBL:GES00396.1,
RC   ECO:0000313|Proteomes:UP000334990};
RA   Ichikawa N., Kimura A., Kitahashi Y., Komaki H., Oguchi A.;
RT   "Whole genome shotgun sequence of Acrocarpospora corrugata NBRC 13972.";
RL   Submitted (OCT-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:GES00396.1}.
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DR   EMBL; BLAD01000044; GES00396.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A5M3VUZ9; -.
DR   Proteomes; UP000334990; Unassembled WGS sequence.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   CDD; cd00091; NUC; 1.
DR   Gene3D; 3.40.570.10; Extracellular Endonuclease, subunit A; 1.
DR   Gene3D; 2.40.10.10; Trypsin-like serine proteases; 2.
DR   InterPro; IPR001604; DNA/RNA_non-sp_Endonuclease.
DR   InterPro; IPR044929; DNA/RNA_non-sp_Endonuclease_sf.
DR   InterPro; IPR020821; Extracellular_endonuc_su_A.
DR   InterPro; IPR044925; His-Me_finger_sf.
DR   InterPro; IPR040255; Non-specific_endonuclease.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   PANTHER; PTHR13966:SF5; ENDONUCLEASE G, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR13966; ENDONUCLEASE RELATED; 1.
DR   Pfam; PF01223; Endonuclease_NS; 1.
DR   Pfam; PF13365; Trypsin_2; 1.
DR   SMART; SM00892; Endonuclease_NS; 1.
DR   SMART; SM00477; NUC; 1.
DR   SUPFAM; SSF54060; His-Me finger endonucleases; 1.
DR   SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
PE   4: Predicted;
KW   Endonuclease {ECO:0000313|EMBL:GES00396.1};
KW   Hydrolase {ECO:0000313|EMBL:GES00396.1};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR640255-2};
KW   Nuclease {ECO:0000313|EMBL:GES00396.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000334990}.
FT   DOMAIN          437..651
FT                   /note="DNA/RNA non-specific endonuclease"
FT                   /evidence="ECO:0000259|SMART:SM00892"
FT   DOMAIN          438..651
FT                   /note="Extracellular Endonuclease subunit A"
FT                   /evidence="ECO:0000259|SMART:SM00477"
FT   REGION          379..400
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          474..494
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        500
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR640255-1"
FT   BINDING         537
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR640255-2"
SQ   SEQUENCE   673 AA;  73411 MW;  F8567BCBF7BE1969 CRC64;
     MDSAGGPATG LRDAAGVRDA AVMADGDGVR AAASARVAGH SQQRRRNIAV LAEPGGMARA
     DEPIRVATRI DRLSRHYRDV RPVSPRAIAA GDPAAIASAE AVLERIIQTD DLLAVGYLEG
     GVAAARAVGR IVIRDQRGRV AGFGTGSLVS PELLLTNHHV LGDAGIAATS GVEFDFQDGV
     DGRPLPLREF GLDPGRFFFA DRELDFALVA VKAGPGELAR FGFNPLVEAE GKAIVGDFVT
     IIQHPRGEKK QIALRENRIV DVLDLFLHYR TDTEPGSSGS PVFNDQWEVV ALHHAGVPAP
     DQAGGVINEG VRVSRLLAML RARPFPPAQQ ALIERLFTGQ AAPSMIPPLP DQPAADGMSP
     QTISLTVPFD VRLRCDRDDA SAPGRTTNGE AVRIDPDYTS REGYDPDFLS PQHQVPLPVL
     HPDLVPLAAG GPGYVLPYHH FSVVINKKRR LAFYTAVNID GATSQRLRRE PDRWTFDPRL
     PEDEQTGESV YRDNPLDRGH LVRRLDPAWG GSRTVAKSAN DDTFHFTNCT PQHQDFNQNQ
     TTWAGLEDYI LENADNRDLR VNVFTGPILA ADDDPYRGVQ LPRQFWKVVA MVTQAGELSA
     TAYLLSQQEL IDGLEIAPER FSYGAYQTYQ VPVRRVEELT RLAFGPLAEA DPFTRTEADA
     PAKEISHFHQ LVL
//