ID A0A5N5GWY2_9ROSA Unreviewed; 1008 AA.
AC A0A5N5GWY2;
DT 26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2020, sequence version 1.
DT 27-MAR-2024, entry version 13.
DE RecName: Full=Zinc protease PQQL-like {ECO:0008006|Google:ProtNLM};
GN ORFNames=D8674_014280 {ECO:0000313|EMBL:KAB2618411.1};
OS Pyrus ussuriensis x Pyrus communis.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Pyrus.
OX NCBI_TaxID=2448454 {ECO:0000313|EMBL:KAB2618411.1, ECO:0000313|Proteomes:UP000327157};
RN [1] {ECO:0000313|EMBL:KAB2618411.1, ECO:0000313|Proteomes:UP000327157}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S2 {ECO:0000313|EMBL:KAB2618411.1};
RC TISSUE=Leaf {ECO:0000313|EMBL:KAB2618411.1};
RA Ou C.;
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000327157}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Wang F., Wang J., Li S., Zhang Y., Fang M., Ma L., Zhao Y., Jiang S.;
RT "A de novo genome assembly of a pear dwarfing rootstock.";
RL Submitted (OCT-2019) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:KAB2618411.1, ECO:0000313|Proteomes:UP000327157}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S2 {ECO:0000313|EMBL:KAB2618411.1};
RC TISSUE=Leaf {ECO:0000313|EMBL:KAB2618411.1};
RA Wang F., Wang J., Li S., Zhang Y., Fang M., Ma L., Zhao Y., Jiang S.;
RT "A de novo genome assembly of a pear dwarfing rootstock.";
RL Submitted (NOV-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the peptidase M16 family.
CC {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAB2618411.1}.
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DR EMBL; SMOL01000401; KAB2618411.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5N5GWY2; -.
DR Proteomes; UP000327157; Chromosome 15.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR InterPro; IPR011249; Metalloenz_LuxS/M16.
DR InterPro; IPR011765; Pept_M16_N.
DR InterPro; IPR001431; Pept_M16_Zn_BS.
DR InterPro; IPR007863; Peptidase_M16_C.
DR PANTHER; PTHR43690; NARDILYSIN; 1.
DR PANTHER; PTHR43690:SF34; ZINC PROTEASE PQQL-LIKE; 1.
DR Pfam; PF00675; Peptidase_M16; 1.
DR Pfam; PF05193; Peptidase_M16_C; 2.
DR SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 3.
DR PROSITE; PS00143; INSULINASE; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000327157};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT TRANSMEM 986..1004
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 57..174
FT /note="Peptidase M16 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00675"
FT DOMAIN 212..392
FT /note="Peptidase M16 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05193"
FT DOMAIN 699..881
FT /note="Peptidase M16 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05193"
SQ SEQUENCE 1008 AA; 113806 MW; 7854E9CACC78A76B CRC64;
MDLLPAETTT SKIVKKHGFR SLKLLTVDMD QELGDQPVGV DYGRLDNGLS YYVRCNSKPR
MRAALALAVK VGSVLEEEDE RGVAHIVEHL AFSATKKYTN HDIVRFLESI GAEFGACQNA
VTSADDTVYE LFVPVDKLEL LSEAISVLAE FSSEVRVSKD DLERERGAVM EEYRGNRNAT
GRMQDAHWIL MMEDSKYAER LPIGLEKVIR TVSPETVKQF YKKWYHLSNM AVIAVGDFSD
TQNVVELIRD QFGRKISAPD PPPIPSYPVP SHEEPRFSCF VESEASGSAV IISYKMAADE
LKTVRDYRDL LAESMFLYAL NQRFFKISRK TDPPYFSCSA SADVLVNPLK AYIMTSSCKE
KGTVEALESM LIEVARVRLH GFSEREVSIV RALLMSEIES AYLERDQMQS TSLRDEYLQH
FLRNEPVIGI EYEAQLQKTL LPHITAAEVS KYAVKLQTSC SCVIKTIEPR ASAIANDLKH
VVSKVNRLEE ERIISPWDEE QIPEEIVNTK PNPGNIVQQV EYSNIEATEL ILSNGMRVCY
KCTNFLDDQV IFTGFSYGGL SELPEREYFS CSMGPTIAGE IGVYGYRPSV LMDMLAGKRA
EVSPKLGAYM RSFVGDCSPS DLETALQLVY QLFTTNIIPG EEDVKIVMQM AEEVVRAQDR
DPYTAFANRV KELNYGNSYF FRPIRISDLR KVDPLKACEY FNKCFKDPST FSVVIVGNID
PSIALPLILQ YLGGIPKPPE PLLQFNRDDL KGLPFNFPKT RIREVVRSPM VEEQCSVQLC
FPVELKNGTM VEDIHIVGFL SKLLETKIMQ VLRFKHGQIY TVGVSVFLGG NKPSRTANVR
GDISVNFSCD PEISSKLVDL TLDEILRLQE EGPSDEDVST ILEIEQRAHE NGLQENYYWL
DRILHSYQSR AYSGDVGTCF EIQDEGRSKV RQSLTPTTAQ SALQRILPFP CKKQYTVVIL
MPRTSPFKSL KSFFQSTESS YQRHTTILAG LAGLTVLGLT LWRYSRRT
//
