ID A0A5N5NLT4_PANHP Unreviewed; 1112 AA.
AC A0A5N5NLT4;
DT 26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2020, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE RecName: Full=Solute carrier family 12 member 2 {ECO:0008006|Google:ProtNLM};
GN ORFNames=PHYPO_G00233710 {ECO:0000313|EMBL:KAB5567521.1};
OS Pangasianodon hypophthalmus (Striped catfish) (Helicophagus hypophthalmus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC Pangasiidae; Pangasianodon.
OX NCBI_TaxID=310915 {ECO:0000313|EMBL:KAB5567521.1, ECO:0000313|Proteomes:UP000327468};
RN [1] {ECO:0000313|EMBL:KAB5567521.1, ECO:0000313|Proteomes:UP000327468}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Indonesia {ECO:0000313|EMBL:KAB5567521.1,
RC ECO:0000313|Proteomes:UP000327468};
RC TISSUE=Blood {ECO:0000313|EMBL:KAB5567521.1};
RA Wen M., Zahm M., Roques C., Cabau C., Klopp C., Donnadieu C., Jouanno E.,
RA Avarre J.-C., Campet M., Ha T.T.T., Dugue R., Lampietro C., Louis A.,
RA Herpin A., Echchiki A., Berthelot C., Parey E., Roest-Crollius H.,
RA Braasch I., Postlethwait J., Bobe J., Montfort J., Bouchez O., Begum T.,
RA Schartl M., Guiguen Y.;
RT "A chromosome-scale genome assembly of the striped catfish, Pangasianodon
RT hypophthalmus.";
RL Submitted (JUN-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 chloride(out) + K(+)(out) + Na(+)(out) = 2 chloride(in) +
CC K(+)(in) + Na(+)(in); Xref=Rhea:RHEA:72395, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72396;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAB5567521.1}.
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DR EMBL; VFJC01000009; KAB5567521.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5N5NLT4; -.
DR OrthoDB; 5490251at2759; -.
DR Proteomes; UP000327468; Chromosome 8.
DR GO; GO:0098590; C:plasma membrane region; IEA:UniProt.
DR GO; GO:0015377; F:chloride:monoatomic cation symporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR002444; NKCC1.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002443; SLC12A1/SLC12A2.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF105; SOLUTE CARRIER FAMILY 12 MEMBER 2-LIKE; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 1.
DR PRINTS; PR01207; NAKCLTRNSPRT.
DR PRINTS; PR01208; NAKCLTRSPRT1.
PE 3: Inferred from homology;
KW Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000327468};
KW Sodium {ECO:0000256|ARBA:ARBA00023053};
KW Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT TRANSMEM 228..258
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 319..338
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 347..366
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 397..416
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 428..447
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 509..533
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 566..586
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 592..610
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 631..660
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 112..163
FT /note="Amino acid permease N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08403"
FT DOMAIN 201..703
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 712..1112
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1112 AA; 121630 MW; FDD559BDCB84DA06 CRC64;
MSDPPSSPPS GQKPTLKTTG TTPSRFQVDR VAEAAAAASG APKTPPGPRS AKAAEGSAGS
GEEAKGRFRV VNFLDPSCAP ADGASDVAFD GDTVRSEASL HSSTGGLSHF SDTHSSTYYL
RTFGHNTIDA VPNIDFYRQT EAPLGEKLIR PSLSELHDEL DKEPFEDGLA NGEEPAAADE
AALKDAPESK GGTVKFGWIK GVLVRCMLNI WGVMLFIRMS WIVGQAGIAL SCAIILIAVL
VTTITGLSTS AIATNGFVRG GGAYYLISRS LGPEFGGSIG LIFAFANAVA VAMYVVGFAE
TVVELLDSVD ALITDEINDI RIVGTLTIIL LLGISMAGME WEAKAQIVLL IILVAAVFNY
FIGSFIPMES KEPKGFFGYN AAIMMENMGP DFRDEETFFS VFAIFFPAAT GILAGANISG
DLSDPQSAIP KGTLLAILIT GLVYVAIAVS NGSCIVRDAT GDANDTVIGS LENCTDAACT
LGYDFSICKE GGCKYGLQND FQVMSLVSAF GPLITAGIFS ATLSSALASL VSAPKVFQAL
CKDNIYPGLR VFAKGYGKNN EPLRGYVLTF IIGLAFILIA ELNVIAPIIS NFFLASYALI
NFSVFHASLA NSPGWRPSFK YFNKWVSLAG AVLCCVVMFV INWWAALLTN VIVLALYIYV
SYKKPDVNWG SSTQALMYNQ ALSHCLHLTG IEDHIKNFRP QCLVMIGAPN ARPALLHLVH
AFTKNMGLMV CGHVRMASRR PNHKEIMNDQ VRYQRWLLKK RIKAFYTPVF AEDLRHGAQY
LLQASGLGRL KPNTMILGFK NNWRDGEMKD VEIYINTIHD AFDIQYGVVI LRLKEGLDIS
HIQGQDEFLS STEKAPGMKE LLVAINIGKD ADDSLKPSSK TTSSQSSPLI IRDTKSPPVQ
LSIADEKLLA ASQQFKKKQC KGTIDVWWLF DDGGLTLLIP YLLTNKKKWK DCKIRVFVGG
KINRIDHDRR AMATLLSKFR IDFSDINVLG DINIKPKKHN KEKFEEMIEP YRLKEDDMEQ
EDAERLKAEE PWRITDNELE LYKAKTNRQI RLNELLKEHS STANLIVMSM PLARKGTVSS
ALYMCWLETL SNGLPPVLLV RGNHQSVLTF YS
//
