ID A0A5P1EML2_ASPOF Unreviewed; 526 AA.
AC A0A5P1EML2;
DT 26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2020, sequence version 1.
DT 27-MAR-2024, entry version 14.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0008006|Google:ProtNLM};
GN ORFNames=A4U43_C06F1010 {ECO:0000313|EMBL:ONK65791.1};
OS Asparagus officinalis (Garden asparagus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Asparagaceae;
OC Asparagoideae; Asparagus.
OX NCBI_TaxID=4686 {ECO:0000313|EMBL:ONK65791.1, ECO:0000313|Proteomes:UP000243459};
RN [1] {ECO:0000313|Proteomes:UP000243459}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. DH0086 {ECO:0000313|Proteomes:UP000243459};
RX PubMed=29093472; DOI=10.1038/s41467-017-01064-8;
RA Harkess A., Zhou J., Xu C., Bowers J.E., Van der Hulst R., Ayyampalayam S.,
RA Mercati F., Riccardi P., McKain M.R., Kakrana A., Tang H., Ray J.,
RA Groenendijk J., Arikit S., Mathioni S.M., Nakano M., Shan H.,
RA Telgmann-Rauber A., Kanno A., Yue Z., Chen H., Li W., Chen Y., Xu X.,
RA Zhang Y., Luo S., Chen H., Gao J., Mao Z., Pires J.C., Luo M., Kudrna D.,
RA Wing R.A., Meyers B.C., Yi K., Kong H., Lavrijsen P., Sunseri F.,
RA Falavigna A., Ye Y., Leebens-Mack J.H., Chen G.;
RT "The asparagus genome sheds light on the origin and evolution of a young Y
RT chromosome.";
RL Nat. Commun. 8:1279-1279(2017).
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
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DR EMBL; CM007386; ONK65791.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5P1EML2; -.
DR EnsemblPlants; ONK65791; ONK65791; A4U43_C06F1010.
DR Gramene; ONK65791; ONK65791; A4U43_C06F1010.
DR OMA; LNCVECP; -.
DR OrthoDB; 2943236at2759; -.
DR Proteomes; UP000243459; Chromosome 6.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004523; F:RNA-DNA hybrid ribonuclease activity; IEA:InterPro.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR CDD; cd22582; BRcat_RBR_unk; 1.
DR CDD; cd22584; Rcat_RBR_unk; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR002156; RNaseH_domain.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR11685:SF466; RING_U-BOX SUPERFAMILY PROTEIN; 1.
DR Pfam; PF01485; IBR; 2.
DR Pfam; PF13456; RVT_3; 1.
DR SMART; SM00647; IBR; 2.
DR SUPFAM; SSF57850; RING/U-box; 2.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000243459};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 203..422
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 207..255
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..56
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 526 AA; 59101 MW; 50B44DF8899575A5 CRC64;
MGSHHDDVEE EFRSCCEDEE WQDTEECLAE LLSSDEEVEE DADDDDDDDG ASSGESEDED
GVSLRVFFKG VSVSEGGGGL GAKASGIGVV METSGGDSVF KVQKKLDFFV DVLVAEHLAL
LDGLIEAQRN GVQRIVALTD NEEVYHQIAE AVPHEDQLLV ALGYRILELS NDFEAFVLKL
VCRSELVRPL KLAREAVGIL DASINQCLVC CCEEKQQSQI IKLKCSHSFC LNCMIVHVEG
KLQALQVPIK CPQVRCKYHI SKNAFKSFLP ISCYQSLERA TMEAKVLNLD RVYCPFPDCS
VLFSPGQHSA SRASSSNQYE INCVECQECC RLLCSSCGVP WHSSMSCEEY QSLSVEERDA
GDANLLRLAQ NNNWRRCQQC RRMVELTQGC FRVTCWCGHE FCYSCSAGYH SGTQTCQCTF
WGDEQESSEP STPSNHEADQ WRWEPFDPLP SITDNYSEQE RAQLALIQRF LAGGFGINDD
HHHNQIQSPP RCSDSYLDAI KDLNQLPWLE RFVSVISDSY HEDYIQ
//
