ID A0A5R8KEM1_9BACT Unreviewed; 484 AA.
AC A0A5R8KEM1;
DT 26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2020, sequence version 1.
DT 08-NOV-2023, entry version 13.
DE RecName: Full=Chromosomal replication initiator protein DnaA {ECO:0000256|HAMAP-Rule:MF_00377, ECO:0000256|RuleBase:RU000577};
GN Name=dnaA {ECO:0000256|HAMAP-Rule:MF_00377,
GN ECO:0000313|EMBL:TLD70711.1};
GN ORFNames=FEM03_10390 {ECO:0000313|EMBL:TLD70711.1};
OS Phragmitibacter flavus.
OC Bacteria; Verrucomicrobiota; Verrucomicrobiae; Verrucomicrobiales;
OC Verrucomicrobiaceae; Phragmitibacter.
OX NCBI_TaxID=2576071 {ECO:0000313|EMBL:TLD70711.1, ECO:0000313|Proteomes:UP000306196};
RN [1] {ECO:0000313|EMBL:TLD70711.1, ECO:0000313|Proteomes:UP000306196}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MG-N-17 {ECO:0000313|EMBL:TLD70711.1,
RC ECO:0000313|Proteomes:UP000306196};
RA Szuroczki S., Abbaszade G., Szabo A., Felfoldi T., Schumann P., Boka K.,
RA Keki Z., Toumi M., Toth E.;
RT "Verrucobacter flavum gen. nov., sp. nov. a new member of the family
RT Verrucomicrobiaceae.";
RL Submitted (MAY-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays an important role in the initiation and regulation of
CC chromosomal replication. Binds to the origin of replication; it binds
CC specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.
CC {ECO:0000256|HAMAP-Rule:MF_00377, ECO:0000256|RuleBase:RU000577}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_00377}.
CC -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000256|ARBA:ARBA00006583,
CC ECO:0000256|HAMAP-Rule:MF_00377, ECO:0000256|RuleBase:RU004227}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TLD70711.1}.
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DR EMBL; VAUV01000007; TLD70711.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5R8KEM1; -.
DR OrthoDB; 9807019at2; -.
DR Proteomes; UP000306196; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003688; F:DNA replication origin binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006270; P:DNA replication initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd00009; AAA; 1.
DR CDD; cd06571; Bac_DnaA_C; 1.
DR Gene3D; 1.10.1750.10; -; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.30.300.180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_00377; DnaA_bact; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR001957; Chromosome_initiator_DnaA.
DR InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR013159; DnaA_C.
DR InterPro; IPR024633; DnaA_N_dom.
DR InterPro; IPR038454; DnaA_N_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010921; Trp_repressor/repl_initiator.
DR NCBIfam; TIGR00362; DnaA; 1.
DR PANTHER; PTHR30050; CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA; 1.
DR PANTHER; PTHR30050:SF2; CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA; 1.
DR Pfam; PF00308; Bac_DnaA; 1.
DR Pfam; PF08299; Bac_DnaA_C; 1.
DR Pfam; PF11638; DnaA_N; 1.
DR PRINTS; PR00051; DNAA.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00760; Bac_DnaA_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF48295; TrpR-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00377};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00377};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_00377};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00377};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00377}; Reference proteome {ECO:0000313|Proteomes:UP000306196}.
FT DOMAIN 178..309
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 391..460
FT /note="Chromosomal replication initiator DnaA C-terminal"
FT /evidence="ECO:0000259|SMART:SM00760"
FT BINDING 186..193
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00377"
SQ SEQUENCE 484 AA; 54795 MW; 582780817029BEDA CRC64;
MEESSAGWYQ GGSKHHLEMM WERISHLLKE RVGGLLYERW LADLQLLSLQ GGKVVFEVPS
IMHECWILDN FMPVLDGVLH EVLGTRCEVE FVAAEAKDRR SSPMPVMEAA RSMESAPVVK
EVVELTAGPL FQGDQRLLKS ISTAGINHRN SFDTFVEGPN CSYSYAVARA VAEKPGKTYN
PLFLHGPVGL GKTHLMQAIG QEILRTKSRK VVRYVTSESF TNEYIDALRH GSVTDFRKKY
RKVDVLLIDD IQFFAGKGST QEEFFHTFND LFNNFKQIVL TSDRAPSEIR NLESRLVSRF
EWGMATQIES PDLETRMAIL KQKTSYWSVP VEDWVLTFLA ENIRTNIRRL EGALMRVAAH
VSLNTGKPLT PPVLEGLLRD ILEEADAKNI TIDWIQKVVA EHFDVRLADM TGKRRPANIA
GPRQLAMYLA RELTKSSLVE IGEAFGGRDH GTVIHACKAV VKRLEQEEFR RLVGQLTEKI
KRIG
//
