ID A0A5R8QIK1_9FIRM Unreviewed; 474 AA.
AC A0A5R8QIK1;
DT 26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2020, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE RecName: Full=Chromosomal replication initiator protein DnaA {ECO:0000256|ARBA:ARBA00018893, ECO:0000256|HAMAP-Rule:MF_00377};
GN Name=dnaA {ECO:0000256|HAMAP-Rule:MF_00377,
GN ECO:0000313|EMBL:TLG77510.1};
GN ORFNames=FEZ08_00735 {ECO:0000313|EMBL:TLG77510.1};
OS Culicoidibacter larvae.
OC Bacteria; Bacillota; Culicoidibacteria; Culicoidibacterales;
OC Culicoidibacteraceae; Culicoidibacter.
OX NCBI_TaxID=2579976 {ECO:0000313|EMBL:TLG77510.1, ECO:0000313|Proteomes:UP000306912};
RN [1] {ECO:0000313|EMBL:TLG77510.1, ECO:0000313|Proteomes:UP000306912}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CS-1 {ECO:0000313|EMBL:TLG77510.1,
RC ECO:0000313|Proteomes:UP000306912};
RA Neupane S., Ghosh A., Gunther S., Martin K., Zurek L.;
RT "Culicoidintestinum kansasii gen. nov., sp. nov. from the gastrointestinal
RT tract of the biting midge, Culicoides sonorensis.";
RL Submitted (MAY-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays an important role in the initiation and regulation of
CC chromosomal replication. Binds to the origin of replication; it binds
CC specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.
CC {ECO:0000256|HAMAP-Rule:MF_00377, ECO:0000256|RuleBase:RU000577}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_00377}.
CC -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000256|ARBA:ARBA00006583,
CC ECO:0000256|HAMAP-Rule:MF_00377, ECO:0000256|RuleBase:RU004227}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TLG77510.1}.
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DR EMBL; VBWP01000001; TLG77510.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5R8QIK1; -.
DR InParanoid; A0A5R8QIK1; -.
DR OrthoDB; 9807019at2; -.
DR Proteomes; UP000306912; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003688; F:DNA replication origin binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006270; P:DNA replication initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd00009; AAA; 1.
DR CDD; cd06571; Bac_DnaA_C; 1.
DR Gene3D; 1.10.1750.10; -; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.30.300.180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_00377; DnaA_bact; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR001957; Chromosome_initiator_DnaA.
DR InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR013159; DnaA_C.
DR InterPro; IPR024633; DnaA_N_dom.
DR InterPro; IPR038454; DnaA_N_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010921; Trp_repressor/repl_initiator.
DR NCBIfam; TIGR00362; DnaA; 1.
DR PANTHER; PTHR30050; CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA; 1.
DR PANTHER; PTHR30050:SF2; CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA; 1.
DR Pfam; PF00308; Bac_DnaA; 1.
DR Pfam; PF08299; Bac_DnaA_C; 1.
DR Pfam; PF11638; DnaA_N; 1.
DR PRINTS; PR00051; DNAA.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00760; Bac_DnaA_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF48295; TrpR-like; 1.
DR PROSITE; PS01008; DNAA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00377};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00377};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_00377};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00377};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00377}; Reference proteome {ECO:0000313|Proteomes:UP000306912}.
FT DOMAIN 169..322
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 384..453
FT /note="Chromosomal replication initiator DnaA C-terminal"
FT /evidence="ECO:0000259|SMART:SM00760"
FT BINDING 177..184
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00377"
SQ SEQUENCE 474 AA; 54925 MW; C43B77B7CC783D0C CRC64;
MIDYAKKTWN MAIEYLLSNQ KITQTSYENW LKEVTPLSMD SQTIIIATRD EFVLQWLSDK
YTNIIENAIE EVIGEKLNVQ FVLENTEPQI EEPSFVTNQN HIQNDFSEVD NINTAYSQTP
QQQFTSQVAP NYELSNLNEF YTFDNFIVGD GNQFSYNISL SVAQNPGKKY NPLFLYGGSG
LGKTHLMQAI GHYILQQNPN TKITYVTCET FLNDFINNII REKNNNEFRR KYRDVDVLLV
DDIQFLAGKE KIQEEFFHTF NHLYSLNKQI VITSDAPPTE LSKLTERLIS RFSWGIITDM
QPLDIETRIA ILNKKVEMLN SDEYFPSDVI EYVASQFELN VRELEGGLNR VLAYASIYGA
RDINLDLAVE ALKGFSKKSS EKMSILRIQK IVAKYFQISV EDLQSKTRKQ PLTTYRQIAI
YLCKELTDNS LQKIGQNFGK RDHTTIMHAV DKIEELRKKD KEIDALIHEL KNQL
//
