ID A0A5S5C5V9_9BACL Unreviewed; 553 AA.
AC A0A5S5C5V9;
DT 26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2020, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE SubName: Full=NOL1/NOP2/sun family putative RNA methylase {ECO:0000313|EMBL:TYP73776.1};
GN ORFNames=BCM02_10652 {ECO:0000313|EMBL:TYP73776.1};
OS Paenibacillus methanolicus.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=582686 {ECO:0000313|EMBL:TYP73776.1, ECO:0000313|Proteomes:UP000323257};
RN [1] {ECO:0000313|EMBL:TYP73776.1, ECO:0000313|Proteomes:UP000323257}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BL24 {ECO:0000313|EMBL:TYP73776.1,
RC ECO:0000313|Proteomes:UP000323257};
RA Whitman W.;
RT "Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the genomes of
RT soil and plant-associated and newly described type strains.";
RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|ARBA:ARBA00007494,
CC ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TYP73776.1}.
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DR EMBL; VNHS01000006; TYP73776.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5S5C5V9; -.
DR OrthoDB; 9810297at2; -.
DR Proteomes; UP000323257; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd21147; RsmF_methylt_CTD1; 1.
DR Gene3D; 2.30.130.60; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR InterPro; IPR031341; Methyltr_RsmF_N.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR027391; Nol1_Nop2_Fmu_2.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR031340; RsmF_methylt_CI.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22807:SF30; 28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF17125; Methyltr_RsmF_N; 1.
DR Pfam; PF13636; Methyltranf_PUA; 1.
DR Pfam; PF17126; RsmF_methylt_CI; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS01153; NOL1_NOP2_SUN; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000323257};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 24..315
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT REGION 315..391
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 344..389
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 235
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 113..119
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 137
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 182
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 553 AA; 60445 MW; 1696D1C4818AAE1E CRC64;
MGITLPQSFA DKMKRLLGPE YEAFLASYER PRKYGLRVNG LKLAPEAFLA MSPLKDRLTP
VPWAEGAFYY EEQDRPGKHP HYHAGLYYIQ EPSAMVPAEL LGVAPGDRVL DLCAAPGGKS
TQLAAKLKGE GVLVTNDNAR ERTKALAKNV ELAGIRNAVV LNEEPAAIAR VFPEWFDKIL
VDAPCSGEGM FRKDESMIAE WEKHSVERCS AMQRDILKHA ADMLAPGGTL VYSTCTFSPE
ENETQIAELL AASPELEVVP VPLAYGWAPG RPAWVEAEAA ASLSEPRLAS IAGTVRLWPH
LTEGEGHYAA VLRKSGEPKD GSLDARSGQS SDAVEVGKLN GDDGGRVGNR RSDTGRPAAN
KREERKRDRE QDRALKSAPK AKDFRSKAAK AALAHAGNPS DAWKQFGAAH LHRLAREGYR
IVAYGTRVYL QPAVVPPLDG LTVVRPGWYV GEAGTYRFAP SHPLAMALSA DETRMSLHYE
AEDADLIRYL KGETLHIEER DLRLRGGAEG DESANPLPSG YVLVCVNDYP VGWGKYSGGG
MLKNELPAGW RWM
//
