ID   A0A653C3X2_CALMS        Unreviewed;       462 AA.
AC   A0A653C3X2;
DT   22-APR-2020, integrated into UniProtKB/TrEMBL.
DT   22-APR-2020, sequence version 1.
DT   27-MAR-2024, entry version 15.
DE   RecName: Full=SAM-dependent MTase RsmB/NOP-type domain-containing protein {ECO:0000259|PROSITE:PS51686};
GN   ORFNames=CALMAC_LOCUS5989 {ECO:0000313|EMBL:VEN42541.1};
OS   Callosobruchus maculatus (Southern cowpea weevil) (Pulse bruchid).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia;
OC   Chrysomeloidea; Chrysomelidae; Bruchinae; Bruchini; Callosobruchus.
OX   NCBI_TaxID=64391 {ECO:0000313|EMBL:VEN42541.1, ECO:0000313|Proteomes:UP000410492};
RN   [1] {ECO:0000313|EMBL:VEN42541.1, ECO:0000313|Proteomes:UP000410492}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Sayadi A.;
RL   Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01023}.
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DR   EMBL; CAACVG010006915; VEN42541.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A653C3X2; -.
DR   Proteomes; UP000410492; Unassembled WGS sequence.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR   InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR   InterPro; IPR049561; NSUN5_7_fdxn-like.
DR   InterPro; IPR048889; NSUN5_RCM1_N.
DR   InterPro; IPR023267; RCMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR22807:SF4; 28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR   Pfam; PF01189; Methyltr_RsmB-F; 1.
DR   Pfam; PF21148; NSUN5_fdxn-like; 1.
DR   Pfam; PF21153; NSUN5_N; 1.
DR   PRINTS; PR02008; RCMTFAMILY.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000410492};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW   ProRule:PRU01023};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01023};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}.
FT   DOMAIN          127..432
FT                   /note="SAM-dependent MTase RsmB/NOP-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51686"
FT   ACT_SITE        362
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         265
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         292
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         310
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ   SEQUENCE   462 AA;  52269 MW;  C0D8A19E835DBDB2 CRC64;
     MFENKQPKRR LARLYKVAAI IAKEYSEGLG SIKQLVYAKR DKHPRIQALY ALVNTLYQRK
     DEIQRLLKKT QLLESESRAD PWVIKILITE LLYGKKVLSG QTKIEQTIKG YEQIFKAHLS
     DSIEVPVKDE TVSTKPKYVR VNTLKKTVEE AIEAFTDEGW HLIRHSDKTD YNGFIEKVAA
     LKDGEFMVDM HVPRLLIFPP RTHFYNHPAY KNGTVILQDK ASCLPVHLLA PPPGSTVLDM
     CAAPGMKTTQ LAAALTNQGV VYAVERDTKR VGILKHIVGS SEATCVKIIN KDVLDCAKKD
     FPGVEYILVD PSCSGSGMTE RSTEAENKDL ARLEKLAGFQ ILILRSALTR YPDAKRVAYS
     TCSLHPEENE EVVRQVLETN YNFKLVDAKK LLNSAWNSFG SSDYGEMGEF CLYARPDEDL
     TNGFFVAIFE RLEEGEQNKF LNNRISNFKN TYMRQTSGKS VG
//