ID A0A671XH42_SPAAU Unreviewed; 1087 AA.
AC A0A671XH42;
DT 17-JUN-2020, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE SubName: Full=Solute carrier family 12 member 2 {ECO:0000313|Ensembl:ENSSAUP00010050409.1};
OS Sparus aurata (Gilthead sea bream).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Spariformes; Sparidae; Sparus.
OX NCBI_TaxID=8175 {ECO:0000313|Ensembl:ENSSAUP00010050409.1, ECO:0000313|Proteomes:UP000472265};
RN [1] {ECO:0000313|Ensembl:ENSSAUP00010050409.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 chloride(out) + K(+)(out) + Na(+)(out) = 2 chloride(in) +
CC K(+)(in) + Na(+)(in); Xref=Rhea:RHEA:72395, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72396;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR AlphaFoldDB; A0A671XH42; -.
DR Ensembl; ENSSAUT00010053022.1; ENSSAUP00010050409.1; ENSSAUG00010013884.1.
DR GeneTree; ENSGT00940000155742; -.
DR Proteomes; UP000472265; Unplaced.
DR GO; GO:0098590; C:plasma membrane region; IEA:UniProt.
DR GO; GO:0015377; F:chloride:monoatomic cation symporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR002444; NKCC1.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002443; SLC12A1/SLC12A2.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF58; SOLUTE CARRIER FAMILY 12 MEMBER 2; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 2.
DR PRINTS; PR01207; NAKCLTRNSPRT.
DR PRINTS; PR01208; NAKCLTRSPRT1.
PE 3: Inferred from homology;
KW Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000472265};
KW Sodium {ECO:0000256|ARBA:ARBA00023053};
KW Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT TRANSMEM 203..222
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 234..263
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 283..304
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 325..344
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 350..371
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 402..420
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 432..451
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 571..591
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 597..614
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 626..644
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 90..142
FT /note="Amino acid permease N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08403"
FT DOMAIN 206..708
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 717..860
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT DOMAIN 856..1087
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 1..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 851..871
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1087 AA; 119181 MW; E3262AE55062B5B1 CRC64;
EASSSDASTT VPPSDKATPT APPDGPGADP GAGGDEAKGR FRVNFADPSE AGSAASPDGA
QAEGLQNGDT VMSETSLHSS TGGQHHYHYD THTNTYYLRT FGHNTIDAVP NIDFYRQTAA
PLGEKLIRPT LSELHDELDK VSRHTWLCPN VSTLCSSHEP DFICCVFQEP FEDGFANGDE
LTPAEEAAAK EAAESKGVVK FGWVKGVLVR CMLNIWGVML FIRMSWIVGQ AGIALSCLIV
GMATVVTTIT GLSTSAIATN GFVRGGGAYY LISRSLGPEF GGSIGLIFAF ANAVAVAMYV
VGFAETVVEL LAGVDAIMTD EINDIRIIGT ITVILLLGIS LAGMEWEAKA QIFLLIVLIT
AICNYFIGSF IPLESKKSMG FFGYDGSIMW ENMGPDFRGE TFFSVFAIFF PAATGILAGA
NISGDLADPQ LAIPRGTLLA ILITGIVYLG VAVSTGSCIV RDASGVLNDT ITQAVGNCSD
AACGLGFDFS SCKSANDCNY GLHNDFQVMS LVSGFGPIIS AGIFSATLSS ALASLVSAPK
VFQALCKDNI YPGLSMFAKG YGKNNEPLKG YILTFVIALA FILIAELNII APIISNFFLA
SYALINFSVF HASLANSPGW RPSFKYYNMW ISLAGAILCC VVMFVINWWA ALLTNVIVLG
LFIYVSYKKP DVNWGSSTQA LTYHQALTHT LHLSGVEDHI KNFRPQCLVM TGYPNSRPAI
IDLVHTFTKN VGLMICGHIR TGYRRPNFKE LATDQARYQR WLLKNECKAF YTPVFAEDMR
QGTQYLLQAA GLGRLKPNTL VLGFKNDWRD GDMMNVETYI SMIHDAFDSQ FGAVILRLKE
GLDVSHIQGQ GMKHMNKHPK HDSRRGFNKK SPTVPLNVSD QRLLEASQQF QKKQGKGTVD
VWWLFDDGGL TLLIPYLLTN KKRWKDCKIR VFIGGKINRI DHDRRAMATL LSKFRIDFSD
ITVLGDINTK PKKEHVSAFE EMIEPYRLKE DDMEQEAAER LKDSEPWRIT DNELELYRAK
TNRQIRLNEL LKEHSSTANL IVMSLPLARK GAVSSALYMA WLEGLSKDLP PMLLVRGNHQ
SVLTFYS
//
