ID A0A6A5CCF9_NAEFO Unreviewed; 1048 AA.
AC A0A6A5CCF9;
DT 17-JUN-2020, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=SAM-dependent MTase RsmB/NOP-type domain-containing protein {ECO:0000259|PROSITE:PS51686};
GN ORFNames=FDP41_010365 {ECO:0000313|EMBL:KAF0983300.1};
OS Naegleria fowleri (Brain eating amoeba).
OC Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia;
OC Vahlkampfiidae; Naegleria.
OX NCBI_TaxID=5763 {ECO:0000313|EMBL:KAF0983300.1, ECO:0000313|Proteomes:UP000444721};
RN [1] {ECO:0000313|EMBL:KAF0983300.1, ECO:0000313|Proteomes:UP000444721}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 30894 {ECO:0000313|EMBL:KAF0983300.1,
RC ECO:0000313|Proteomes:UP000444721};
RX PubMed=31690847;
RA Liechti N., Schurch N., Bruggmann R., Wittwer M.;
RT "Nanopore sequencing improves the draft genome of the human pathogenic
RT amoeba Naegleria fowleri.";
RL Sci. Rep. 9:0-16040(2019).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF0983300.1}.
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DR EMBL; VFQX01000006; KAF0983300.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A6A5CCF9; -.
DR VEuPathDB; AmoebaDB:FDP41_010365; -.
DR VEuPathDB; AmoebaDB:NF0126250; -.
DR VEuPathDB; AmoebaDB:NF0126260; -.
DR VEuPathDB; AmoebaDB:NF0126270; -.
DR VEuPathDB; AmoebaDB:NfTy_011600; -.
DR OrthoDB; 5478193at2759; -.
DR Proteomes; UP000444721; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22808; NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR22808:SF1; RNA CYTOSINE C(5)-METHYLTRANSFERASE NSUN2; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 2.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000444721};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 299..738
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT REGION 1..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 251..271
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 545..573
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..61
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 71..105
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 613
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 401..407
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 427
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 484
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 1048 AA; 118548 MW; 9C5BBE341C03AD2D CRC64;
MSSQNTLYDP SDVSVLGKRK TVSGDDSSLN EEEGSSLNNN TTTTTPSDPS QKKVKSSSLS
TNDEQKFDDT RGASSMNNTI NNNNTITDNT NNNTINNNIT NNTNKRTNYD EIIPENEMFY
KYYYEIQKIV QSKEEMDQMM QYFQQPLPLT FRINKSLPLE LIQKIKSQLS EFKTQLNHYK
YDEETEKVRS MTDIESKGVQ IDIIHLNHIN DLFYQLCPNI SKRKLRRQNS ACGMSVVDVG
KVNSSVNDNT TFNATPNSTT TPNVTAATTP TRTDSGAAEY VNLDEINGQD LNDLQDDMIM
TTTLDESEGS TLLTSFRDYL VKLTTIGYIS RQELVSMIPP LCFTPAIMTT TTTTTIPTTT
TIPTLDTTTT TTTTQQQQLE SKELMETEMA MTWNDGYVDM CSAPGSKSAQ MVEYYLNGKD
KFLICNDIDR KRLTTLFSNL NREPQQLTSN IVVTHHNAVT LHQVLASSTI QGHALSHVHH
VLCDAVCSGD GTLRKSRDLW ERWNYEHGHV NHMTQVSILV SAMNIIQTSF NQEQEEKAVL
VTTTTSTTTT IEKSSNQSSG GDSSQSGSIQ NDSIGSIQND SIGSIGSIGS GDSGSAILSN
TYHGEYEIVY STCSLNPIEN EAVVAEAIRR TNHRFELVNL EETFSNSISY RKGMNDWKVV
DRNGQVVNSF EECILEGDFI PRSGSKSDNR PKKETCSRIS ASMFPKEDVK SMNLHYTMRI
LPHLNNSGGF YVAKLRLKKE FLMNDEEFKT RRQRKFEKKL EKKKHLQQKQ TPNKKNVIYV
KALKEHVQPL LDFYQIDLTR MMNHSNNTTD QVRIDQVRID QVRIDQVRTD SDTLIRNHFL
VQTTPESKYA QAILKLSQVN PTNSDTITNS STTTSATTST TIAPPKKFTF MKNSYLCEYL
RTNFSPEESL NMKIFHSGIV AFEDSKDSKV FQITLGGALS LIDCMDPNST QLMEITKMQM
KHLLTDGKLP LGDSRFIQNE ELEKKQLEKL KEMKMGGCVL KCMMVDSSDH KKYLLAVGLK
YNANTIFNRT EREEAANLLI KLDYDFYY
//
