ID A0A6A6L395_HEVBR Unreviewed; 302 AA.
AC A0A6A6L395;
DT 17-JUN-2020, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 1.
DT 27-MAR-2024, entry version 13.
DE RecName: Full=feruloyl-CoA 6-hydroxylase {ECO:0000256|ARBA:ARBA00012885};
DE EC=1.14.11.61 {ECO:0000256|ARBA:ARBA00012885};
GN ORFNames=GH714_007824 {ECO:0000313|EMBL:KAF2294139.1};
OS Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Micrandreae;
OC Hevea.
OX NCBI_TaxID=3981 {ECO:0000313|EMBL:KAF2294139.1, ECO:0000313|Proteomes:UP000467840};
RN [1] {ECO:0000313|EMBL:KAF2294139.1, ECO:0000313|Proteomes:UP000467840}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. GT1 {ECO:0000313|Proteomes:UP000467840};
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF2294139.1};
RX PubMed=31838037; DOI=10.1016/j.molp.2019.10.017;
RA Liu J., Shi C., Shi C.C., Li W., Zhang Q.J., Zhang Y., Li K., Lu H.F.,
RA Shi C., Zhu S.T., Xiao Z.Y., Nan H., Yue Y., Zhu X.G., Wu Y., Hong X.N.,
RA Fan G.Y., Tong Y., Zhang D., Mao C.L., Liu Y.L., Hao S.J., Liu W.Q.,
RA Lv M.Q., Zhang H.B., Liu Y., Hu-Tang G.R., Wang J.P., Wang J.H., Sun Y.H.,
RA Ni S.B., Chen W.B., Zhang X.C., Jiao Y.N., Eichler E.E., Li G.H., Liu X.,
RA Gao L.Z.;
RT "The Chromosome-Based Rubber Tree Genome Provides New Insights into Spurge
RT Genome Evolution and Rubber Biosynthesis.";
RL Mol. Plant 13:336-350(2020).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-feruloyl-CoA + 2-oxoglutarate + O2 = (E)-6-
CC hydroxyferuloyl-CoA + CO2 + succinate; Xref=Rhea:RHEA:57856,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810,
CC ChEBI:CHEBI:30031, ChEBI:CHEBI:87305, ChEBI:CHEBI:142390;
CC EC=1.14.11.61; Evidence={ECO:0000256|ARBA:ARBA00000471};
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC Evidence={ECO:0000256|ARBA:ARBA00001961};
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. {ECO:0000256|RuleBase:RU003682}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF2294139.1}.
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DR EMBL; JAAGAX010000013; KAF2294139.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A6A6L395; -.
DR Proteomes; UP000467840; Chromosome 7.
DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProt.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR PANTHER; PTHR10209:SF243; FERULOYL COA ORTHO-HYDROXYLASE 1-RELATED; 1.
DR PANTHER; PTHR10209; OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN; 1.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU003682};
KW Metal-binding {ECO:0000256|RuleBase:RU003682};
KW Oxidoreductase {ECO:0000256|RuleBase:RU003682};
KW Reference proteome {ECO:0000313|Proteomes:UP000467840}.
FT DOMAIN 139..251
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
SQ SEQUENCE 302 AA; 33835 MW; 2481C525415B1AA6 CRC64;
MSNWENDPKI AESVCEAAEK FGFFQLVNHG VPLEVLEGVK DATHRFFGLP AAVKRKYSKE
LSPSNSVRFG TSFSPDSEKA LEWKDYLSLF YVSEEEASAL WPTECKDEVL EYMKKSQVLC
TKLMTALMEK LNVKEIDETK ESLLMGSKRI NLNYYPRCPN PQLTVGVGRH SDVSSLTFLL
QDEIGGLYVR VNEGKGEEDG WVHVPPIEGS LVINVGDALQ ILSNGRYKSV EHCVIASGSK
NRISIPIFVN PKPNDVIGPL PELIAAGEKP KYKNILYSDY VKHFFRKAHD GKKTVAFAEI
QS
//
