ID   A0A6I0FGN0_9CORY        Unreviewed;      1104 AA.
AC   A0A6I0FGN0;
DT   12-AUG-2020, integrated into UniProtKB/TrEMBL.
DT   12-AUG-2020, sequence version 1.
DT   24-JAN-2024, entry version 12.
DE   SubName: Full=CRISPR-associated protein {ECO:0000313|EMBL:KAB3538337.1};
GN   ORFNames=F8390_08650 {ECO:0000313|EMBL:KAB3538337.1};
OS   Corynebacterium sp. 366.
OC   Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
OC   Corynebacteriaceae; Corynebacterium.
OX   NCBI_TaxID=2652251 {ECO:0000313|EMBL:KAB3538337.1, ECO:0000313|Proteomes:UP000439070};
RN   [1] {ECO:0000313|EMBL:KAB3538337.1, ECO:0000313|Proteomes:UP000439070}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=366 {ECO:0000313|EMBL:KAB3538337.1,
RC   ECO:0000313|Proteomes:UP000439070};
RA   Zhang C.;
RT   "Corynebacterium sp novel species isolated from the respiratory tract of
RT   Marmot.";
RL   Submitted (OCT-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KAB3538337.1}.
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DR   EMBL; WBZK01000003; KAB3538337.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A6I0FGN0; -.
DR   Proteomes; UP000439070; Unassembled WGS sequence.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.30.50; -; 1.
DR   Gene3D; 3.30.70.3520; -; 1.
DR   Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 3.
DR   InterPro; IPR040619; Cas9_alpha-helical_lobe.
DR   InterPro; IPR040796; Cas9_b_hairpin.
DR   InterPro; IPR041217; Cas9_C.
DR   InterPro; IPR041225; Cas9_Topo.
DR   InterPro; IPR002711; HNH.
DR   InterPro; IPR033114; HNH_CAS9.
DR   InterPro; IPR003615; HNH_nuc.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR041383; RuvC_III.
DR   Pfam; PF18470; Cas9_a; 1.
DR   Pfam; PF17893; Cas9_b_hairpin; 1.
DR   Pfam; PF18525; Cas9_C; 1.
DR   Pfam; PF17894; Cas9_Topo; 1.
DR   Pfam; PF01844; HNH; 1.
DR   Pfam; PF18541; RuvC_III; 1.
DR   SMART; SM00507; HNHc; 1.
DR   PROSITE; PS51749; HNH_CAS9; 1.
PE   4: Predicted;
KW   Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PROSITE-
KW   ProRule:PRU01085}; Endonuclease {ECO:0000256|PROSITE-ProRule:PRU01085};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU01085}; Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Nuclease {ECO:0000256|PROSITE-ProRule:PRU01085};
KW   Reference proteome {ECO:0000313|Proteomes:UP000439070};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT   DOMAIN          520..681
FT                   /note="HNH Cas9-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51749"
SQ   SEQUENCE   1104 AA;  124318 MW;  CD37D07C8B919C9F CRC64;
     MNAVQSTKPK NYRVGIDVGL RSIGFAAIEV DEQGSPLQIL NAMSLIHDAG LDPDSQKTAQ
     TRLMVSGVAR RTRRLYRQRK RRLKELDEFL AKNGYPLPDL TTSEEKIGWK SRADLATAKI
     EDPEELKLAL SHAARHIARH RGWRNPYTTV KSMMNKAGEH SDGFLAVEET FRELIGEQKH
     AGQSLTLAQM VMALPNGTTK MRGTGGLLSE RLQQKDFVKE MLLIGEVQGL DEEFLEKLIE
     RIFYAKSPKG SAAGRVGKDL LDPQEFRAWR ATRAFQEYRI ISLLANVRIQ ERVGTSLGEI
     RPLSVEERQR VFTLLNTWTD SPLPTWANVA EELGVDRGDL RGTASSNDDG ERVSANPPVN
     QVEVVMCSTV IKEFKKFWET ANNPSKDALI RELSNVEAPS EDSPEAIAAS SLVRSLPPKS
     LEKLESLKLP DGRAAYSVKT LEKLRDYMLN NVADLYTARQ SLFDLPNDWA PPAEPIGAPT
     GNPAVDRVLK ATNRWLLLAE KRWGAPLSIN IESLRDGFKS ESAAREIQRE QETRAKNNQA
     IVQTMMQKLG VDGRPSRNDV LRYQSIQRQN GQCAYCGTTI TMKTSEMDHI VPRAGVGSTN
     SRYNLLAVCA NCNRSKGKLP FAVWAKKSNN PQVSLEAAIE RVRHWPTDAG MRQKQMNDFK
     QQVIFRLKRV SEDEEIDARS KESVSWMANE LRHRVSHHYQ EAVKVRVFRG SVTAGARRAS
     GIEDRLKLIG GRSGKNRLDR RHHAVDAAVI ALMQATVAQV LAERDSLRSE YRTTRQVDTT
     YGHWRDYTGK TIADQRTFKV WTQRMLTLVD LLQTAIDEDR IPVTENIRLK LGNGLAHEET
     IKPLKKVLLK SELSMEDIDR AATPALWAAL TRLPDFDWKK GLPENPDREI SVNGTHIGPN
     DHIELFGVKA AALALNGGYV ELGASFHHAR LYRIKGAKQD SYAMMRVYTN DLLRYRHEDL
     FSVELPPHTM SMRQAEPKLR TALRAGKAEY VTWFVVGDEL IFDTEKLATG QISRYLKEFG
     PTKHWRIRGL NTDIQLRLRP AQLSAEGLTS ETSSDSKKII DNPGWRPTVN KIFSAGNVTL
     IRRDIHGRVR ITDHNGLPTS VKIS
//