ID A0A6I1G0Q4_9MICO Unreviewed; 1245 AA.
AC A0A6I1G0Q4;
DT 12-AUG-2020, integrated into UniProtKB/TrEMBL.
DT 12-AUG-2020, sequence version 1.
DT 08-NOV-2023, entry version 13.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN ORFNames=GA707_10220 {ECO:0000313|EMBL:KAB7743858.1};
OS Tetrasphaera sp. F2B08.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC Tetrasphaera.
OX NCBI_TaxID=2653936 {ECO:0000313|EMBL:KAB7743858.1, ECO:0000313|Proteomes:UP000437955};
RN [1] {ECO:0000313|EMBL:KAB7743858.1, ECO:0000313|Proteomes:UP000437955}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F2B08 {ECO:0000313|EMBL:KAB7743858.1,
RC ECO:0000313|Proteomes:UP000437955};
RA Feng X.;
RT "Tetrasphaera sediminis sp.nov.";
RL Submitted (OCT-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAB7743858.1}.
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DR EMBL; WESE01000004; KAB7743858.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A6I1G0Q4; -.
DR Proteomes; UP000437955; Unassembled WGS sequence.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000313|EMBL:KAB7743858.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000437955}.
FT DOMAIN 52..116
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 1245 AA; 138496 MW; DC86CCCD4A36EA33 CRC64;
MNSPRIDGPL SEALVGTRRY FTRGEVSDDQ RTMHLEGGRQ GDSFYRDRWS HDKVVRSTHG
VNCTGSCSWK VYVKDGIITW EAQQTDYPSA GADRPEYEPR GCPRGAAFSW YTYSPTRVRY
PYVRGVLLEM FREAKAAVGG DPVVAWASIV QDPEKAHRYK SARGKGGLVR STWDEVVELV
AAAHVYTVKR WGPDRIAGFS PIPAMSPVSY ASGARFLELI GAPMLSFYDW YADLPNASPQ
MFGDQTDVPE SGDWWDAGYL MMWGSNVPLT RTPDAHWMTE ARYRGQKVVA VAPDYAENVK
FADEWVTCNP GADGALALAM GHVILKEYFV DRQVQFFVDY NRRYTDLPFL VTLDEVSEGS
EDGTDGTTAY RPGKNLVAAD FDNRQRESEN AVFKPVVFDG ATGRPAVPNG SIGFRYGDEG
WGKWNLDLEG IEPVLTMYAG GTSTSDPASG VAEVVLPRFD TADGHVTYVR RGVPTRRVGG
RLVTTVLDLM LAQYGVSRPG LPGEWPADYD DASTPATPAW QEPITGVPAQ QVTRLAREWA
QNAIDTTGRG MILMGAGTNH WFHSDQIYRA MLVLTTITGC QGRNGGGWAH YVGQEKIRPI
MGFQHMAFAL DWHRPPRHMN QTAYWYVNTS QYRYDTFTAD DLDAGTGVFA DKGVMDLLAQ
SVRLGWTPSY PTFTRSSLVL ADEARAAGQE PIPYVVDQLK SGELKFAVED PEAEENHPRI
LSLWRANLLG SSAKGNEYFL KHLLGADNAV SAAEAPPEKR PAGIVWPEHA PEGKLDLLTT
LDFRMTSSTL FSDVVLPAAT WYEKHDLNTT DMHPFVHAFS PAIAPPWQSK TDWDTWKAVA
KKFSELAVDH LGTRTDVVAK PLWHDTPEAM ATPHGIVKDW RTGEVEPVPG RTLPVLALAE
RDYPAVYDKM TSIGPLLEKV GMLTKGVPYD VTREVDILRG RNGVRNGGAG DGQPRLETDI
HMADAILHLS GTANGHLATH GFRNVEKRTG TQLHDLAAEH EGKQITFADT QVQPVPVITS
PEWSGSESGG RRYSPFTINV ERLKPFHTLT GRQQFYLDHD WILGMGEALP VYRPPLNMTT
LFGESEIGVQ NELGVSVRYL TPHNKWSIHS EYQDNLFMLS LSRGGQTIWM SDRDAAKVGI
KDNDWVEAVN RNGVVAARAI VSHRMPEGTI FMHHAQDRLI DVPLTETNQR RGGIHNSLTR
ILIKPSHIIG GYAQLAYFFN YIGPTGNNRD EVTMIRRRTD QNVTY
//
