ID   A0A6I4TTE7_9SPHN        Unreviewed;      1014 AA.
AC   A0A6I4TTE7;
DT   12-AUG-2020, integrated into UniProtKB/TrEMBL.
DT   12-AUG-2020, sequence version 1.
DT   27-MAR-2024, entry version 12.
DE   SubName: Full=Insulinase family protein {ECO:0000313|EMBL:MXO98490.1};
GN   ORFNames=GRI97_05750 {ECO:0000313|EMBL:MXO98490.1};
OS   Croceibacterium xixiisoli.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC   Erythrobacteraceae; Croceibacterium.
OX   NCBI_TaxID=1476466 {ECO:0000313|EMBL:MXO98490.1, ECO:0000313|Proteomes:UP000469430};
RN   [1] {ECO:0000313|EMBL:MXO98490.1, ECO:0000313|Proteomes:UP000469430}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S36 {ECO:0000313|EMBL:MXO98490.1,
RC   ECO:0000313|Proteomes:UP000469430};
RA   Xu L.;
RT   "Genomic-based taxomic classification of the family Erythrobacteraceae.";
RL   Submitted (DEC-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947};
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:MXO98490.1}.
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DR   EMBL; WTYJ01000001; MXO98490.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A6I4TTE7; -.
DR   Proteomes; UP000469430; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 3.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000469430};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          127..256
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          283..463
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          758..937
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   REGION          73..93
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1014 AA;  110484 MW;  F6ECD3E236BB7690 CRC64;
     MTAARAKCSI CPRTAASCAR ARPSPRSKRF GSNGPRLVVA GSAFLGQTPG MLRLLTAVPL
     IFLTNPALAR DAGQADPMPP VQSQAAAQTA APVQPPVWPM DGSDLPVDPA YRFGVLDNGL
     RYIIRPNGTP AAQGMVQFWI EGGSIDETEA ERGYAHFVEH MAFNGSTRVP EGEMIHLLER
     QGLAFGADTN ASTSFDTTLY KLDLPRNDPA LLDTALMLMR EVASELLFDP AAVEREKGVV
     LSEMRVRDSY AMDNTVERMK FLFPTALFPD RLPIGKAETL QAATAESLRA LWQRLYRPGN
     SALIVVGDYD PDVVEAAIRA HFSSWQAPPA LPPVSEGPID VDLAGKTDIF LHPALSERIT
     ITRHGEWIGE PDTVAYRQRN VRRQIGYGII NRRFQSMARE EDPPFRGAGL GSSEAFQIGR
     STNLVIDAGE GEWKRGLAAA QAEYRRALAF GFTEAEVAEQ LANLRSALEN NVAGADTRGN
     ASFITGALTL LEDGQIPTTP ASGLARFEDH VPLITPQSVL ESLQEELIPL DNPLIRFEGR
     TAPEGGADAL RAAWNAGMDI QPTRGDRAET AEFAYSDFGP AGTVVSDQVD PRYGIRKLVF
     ANGLMLNIKR TNLQRGRVGV ELNVDGGQML NTREKPLATA MAGVLPMGGL GRHSNDELQS
     VLAGRTVGFN FETDERSFRL AAQTTPRDLL LQLQLLTAGL TDPGYRPQGE AQYRRNIRNV
     FARRTATPDN ALSASLGEIV SDGDPRFTRP EAEAYLALDF EQLRDTISDR LDHGALELAL
     VGDLDEEAAI AAVASTLGAL PTREASFRPY SENRERSFTA DRSVRTLYHD GTAQQALLRM
     SWPTTDDTDL RTMVTLNLLE RIVRLELTAK LREELGQTYS PSVSSSQSST YRGYGLFTIA
     AAVDVSQVAA AREAMLETIG ATLAGPLDDD LLLRARAPLL ERYDSALKSN GGWMNLTRRA
     QSRPEIVDRF TQARDILAGL TLEDVKTVAA RFLDTQNQLE VDVLPRGMAS SAAE
//