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Database: UniProt
Entry: A0AL18
LinkDB: A0AL18
Original site: A0AL18 
ID   ADDA_LISW6              Reviewed;        1235 AA.
AC   A0AL18;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2006, sequence version 1.
DT   10-OCT-2018, entry version 76.
DE   RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE   AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   Name=addA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   OrderedLocusNames=lwe2282;
OS   Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 /
OS   SLCC5334).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=386043;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35897 / DSM 20650 / SLCC5334;
RX   PubMed=16936040; DOI=10.1128/JB.00758-06;
RA   Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R.,
RA   Chatterjee S.S., Domann E., Kaerst U., Goesmann A., Bekel T.,
RA   Bartels D., Kaiser O., Meyer F., Puehler A., Weisshaar B., Wehland J.,
RA   Liang C., Dandekar T., Lampidis R., Kreft J., Goebel W.,
RA   Chakraborty T.;
RT   "Whole-genome sequence of Listeria welshimeri reveals common steps in
RT   genome reduction with Listeria innocua as compared to Listeria
RT   monocytogenes.";
RL   J. Bacteriol. 188:7405-7415(2006).
CC   -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA
CC       helicase and an ATP-dependent, dual-direction single-stranded
CC       exonuclease. Recognizes the chi site generating a DNA molecule
CC       suitable for the initiation of homologous recombination. The AddA
CC       nuclease domain is required for chi fragment generation; this
CC       subunit has the helicase and 3' -> 5' nuclease activities.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC   -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC       Rule:MF_01451}.
CC   -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
DR   EMBL; AM263198; CAK21700.1; -; Genomic_DNA.
DR   RefSeq; WP_011703031.1; NC_008555.1.
DR   SMR; A0AL18; -.
DR   STRING; 386043.lwe2282; -.
DR   PRIDE; A0AL18; -.
DR   EnsemblBacteria; CAK21700; CAK21700; lwe2282.
DR   KEGG; lwe:lwe2282; -.
DR   eggNOG; ENOG4107R2I; Bacteria.
DR   eggNOG; COG1074; LUCA.
DR   HOGENOM; HOG000015621; -.
DR   KO; K16898; -.
DR   OMA; KQSIYRW; -.
DR   OrthoDB; POG091H004Y; -.
DR   BioCyc; LWEL386043:G1GJF-2348-MONOMER; -.
DR   Proteomes; UP000000779; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.320.10; -; 1.
DR   HAMAP; MF_01451; AddA; 1.
DR   InterPro; IPR014152; DNA_helicase_suAddA.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR011604; Exonuc_phg/RecB_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038726; PDDEXK_AddAB-type.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   InterPro; IPR034739; UvrD/AddA_N.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   PANTHER; PTHR11070:SF14; PTHR11070:SF14; 1.
DR   Pfam; PF12705; PDDEXK_1; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 4.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding;
KW   Exonuclease; Helicase; Hydrolase; Nuclease; Nucleotide-binding.
FT   CHAIN         1   1235       ATP-dependent helicase/nuclease subunit
FT                                A.
FT                                /FTId=PRO_0000379299.
FT   DOMAIN       12    482       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   DOMAIN      509    800       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   NP_BIND      33     40       ATP. {ECO:0000255|HAMAP-Rule:MF_01451}.
SQ   SEQUENCE   1235 AA;  142654 MW;  376BBA73D4D3034A CRC64;
     MGLNIPEKPV NTLWTDDQWK AIQANGNNIL VAAAAGSGKT AVLVTRIIEK LVDETGNLNV
     DELLIVTFTN ASAAEMKYRI GKSLEEALSQ NPDSSHLKKQ VALLNYASIS TLHSFCLEII
     RKHYFEADID PNFRLIEPIE SSMIRDEVLE ELLEKEYSIA NNEAFFHLVE SFTGDRTDAE
     LHMLISKLYD FSRANPNPDL WLEQMVNFYD TKDINSITEL PYFPIIKEDI ELRINQAKSY
     LLNAIEYASE NNGPAPYLET LENDLAQINT LSNISWTNWQ DVKLRVESMD FKRIPSLKNK
     SDYDEEYVEE TKRFRDAAKK EIKNVLVDWF SREETNYLAD LEKMKPDIKT ISELVKNFAN
     NFFEEKQRRG VLDFNDLEHL ALKILLKNDV PSDVAKSYQK QFKEVLIDEY QDTNMVQETI
     LLLVTNSEES KGNLFMVGDV KQSIYRFRLA EPTLFMTKYQ EYQQNGEGEG IRIDLSQNFR
     SRKEVLDATN FIFHQLMDKH VAEIDYDEAA ELTLGANFPK SNHMATELLL IDMKSNENES
     EDELSPQELQ KNQVEARAIA TKIREMIDNK FPIYDKKLQQ NRSIQYRDIV ILSRAMTSAP
     DMEEAMKVQD IPFYASNNSG YFETTEVATM IALLKVIDNP YQDIPLAAVL RSPIVGLNEE
     ELGQIRMAKK KGYFFDALLA YKDITVSAAA DRISDFITQL NNWRELSIRE NLTALIWQIY
     QETNFYEFVG GLPGGKQRQA NLRALYDRAN QYEKTAFRGL FRFVRFVERL EVRGDDLGTA
     KTLGEKEDVV RMMTIHASKG LEFPVVIISG LSKKFNMRDI YSKTLLDKDY GFASNYRDIE
     KMIVYPTIMQ QAIKQKKSRE MIAEEMRVLY VALTRAEEKL ILTATVPDFE KTSKNWLQVS
     NQKETILPAS IRAKAKCYLD WIGNTIIRHT SFKDLLCEER IQTLPTEMKL QIEIKTKEMF
     LTTELEEHKA DNWLENVKAH EPVPVQSAYK DEIERFMNYK YKDVAATEIR AKQSVTELKR
     QFSLQDSWSD TSILKEFQKV SLDRPKFLQQ NKLSATEIGT AMHTLMQAVS LTYKPSEKDL
     TSLLQSMQEK DILTEAQIKA INIKQIMGFF DSPLGETVLQ KSDQVKREVP FSYLLPVAKL
     YKQSDLEEHV LIQGVVDSMI EEEDAITLID YKTDKIEGRY ANWEAAEKVM KERYQIQIKL
     YAEAIQAITG KKVSNAYLYF FDGQHICQIN IEEGF
//
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