ID A0CUD3_PARTE Unreviewed; 972 AA.
AC A0CUD3;
DT 28-NOV-2006, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2006, sequence version 1.
DT 27-MAR-2024, entry version 81.
DE RecName: Full=Glycine cleavage system P protein {ECO:0000256|RuleBase:RU364056};
DE EC=1.4.4.2 {ECO:0000256|RuleBase:RU364056};
GN ORFNames=GSPATT00010600001 {ECO:0000313|EMBL:CAK74400.1};
OS Paramecium tetraurelia.
OC Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC Oligohymenophorea; Peniculida; Parameciidae; Paramecium.
OX NCBI_TaxID=5888 {ECO:0000313|EMBL:CAK74400.1, ECO:0000313|Proteomes:UP000000600};
RN [1] {ECO:0000313|EMBL:CAK74400.1, ECO:0000313|Proteomes:UP000000600}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Stock d4-2 {ECO:0000313|EMBL:CAK74400.1,
RC ECO:0000313|Proteomes:UP000000600};
RX PubMed=17086204; DOI=10.1038/nature05230;
RG Genoscope;
RA Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M.,
RA Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A.,
RA Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R.,
RA Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F.,
RA Le Moue A., Lepere C., Malinsky S., Nowacki M., Nowak J.K., Plattner H.,
RA Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M.,
RA Weissenbach J., Scarpelli C., Schachter V., Sperling L., Meyer E.,
RA Cohen J., Wincker P.;
RT "Global trends of whole-genome duplications revealed by the ciliate
RT Paramecium tetraurelia.";
RL Nature 444:171-178(2006).
CC -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC glycine. {ECO:0000256|RuleBase:RU364056}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glycine + H(+) + N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage
CC complex H protein] = CO2 + N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-
CC L-lysyl-[glycine-cleavage complex H protein]; Xref=Rhea:RHEA:24304,
CC Rhea:RHEA-COMP:10494, Rhea:RHEA-COMP:10495, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:57305, ChEBI:CHEBI:83099,
CC ChEBI:CHEBI:83143; EC=1.4.4.2;
CC Evidence={ECO:0000256|ARBA:ARBA00043839,
CC ECO:0000256|RuleBase:RU364056};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000256|ARBA:ARBA00001933,
CC ECO:0000256|PIRSR:PIRSR603437-50, ECO:0000256|RuleBase:RU364056};
CC -!- SUBUNIT: The glycine cleavage system is composed of four proteins: P,
CC T, L and H. {ECO:0000256|RuleBase:RU364056}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|RuleBase:RU364056}.
CC -!- SIMILARITY: Belongs to the GcvP family. {ECO:0000256|ARBA:ARBA00010756,
CC ECO:0000256|RuleBase:RU364056}.
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DR EMBL; CT868185; CAK74400.1; -; Genomic_DNA.
DR RefSeq; XP_001441797.1; XM_001441760.1.
DR AlphaFoldDB; A0CUD3; -.
DR STRING; 5888.A0CUD3; -.
DR EnsemblProtists; CAK74400; CAK74400; GSPATT00010600001.
DR GeneID; 5027582; -.
DR KEGG; ptm:GSPATT00010600001; -.
DR eggNOG; KOG2040; Eukaryota.
DR HOGENOM; CLU_004620_3_2_1; -.
DR InParanoid; A0CUD3; -.
DR OMA; DEHCHPQ; -.
DR Proteomes; UP000000600; Partially assembled WGS sequence.
DR GO; GO:0005960; C:glycine cleavage complex; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0016594; F:glycine binding; IBA:GO_Central.
DR GO; GO:0004375; F:glycine dehydrogenase (decarboxylating) activity; IBA:GO_Central.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IBA:GO_Central.
DR GO; GO:0019464; P:glycine decarboxylation via glycine cleavage system; IBA:GO_Central.
DR CDD; cd00613; GDC-P; 1.
DR Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 2.
DR Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 2.
DR InterPro; IPR003437; GcvP.
DR InterPro; IPR049316; GDC-P_C.
DR InterPro; IPR049315; GDC-P_N.
DR InterPro; IPR020581; GDC_P.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR NCBIfam; TIGR00461; gcvP; 1.
DR PANTHER; PTHR11773:SF1; GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL; 1.
DR PANTHER; PTHR11773; GLYCINE DEHYDROGENASE, DECARBOXYLATING; 1.
DR Pfam; PF21478; GcvP2_C; 1.
DR Pfam; PF02347; GDC-P; 2.
DR SUPFAM; SSF53383; PLP-dependent transferases; 2.
PE 3: Inferred from homology;
KW Mitochondrion {ECO:0000256|RuleBase:RU364056};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU364056};
KW Pyridoxal phosphate {ECO:0000256|PIRSR:PIRSR603437-50,
KW ECO:0000256|RuleBase:RU364056};
KW Reference proteome {ECO:0000313|Proteomes:UP000000600};
KW Transit peptide {ECO:0000256|RuleBase:RU364056}.
FT DOMAIN 40..464
FT /note="Glycine cleavage system P-protein N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02347"
FT DOMAIN 478..755
FT /note="Glycine cleavage system P-protein N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02347"
FT DOMAIN 792..912
FT /note="Glycine dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF21478"
FT MOD_RES 726
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000256|PIRSR:PIRSR603437-50"
SQ SEQUENCE 972 AA; 108867 MW; EEE5FEBD92A12AF3 CRC64;
MLRSIRLIQR VANFSRLANP HEVNPKVPDW FKSSDYMEPR FIGSESQQVN EMLKAVEAKS
LDELVDKIIP KEIRSEAAFQ SPDNFPDAIP ESAMVQHLQS LANKNKLYKN YIGQGFYGTH
TPYVILRNVL EDPGWYTSYT PYQAEISQGR LEALLNYQTV ITELTGMDVS NASLLDEATA
AGEAMFLANS WFEKKKFFVD NHVFPQSIDH IKTKAYYLGI DIVVGDAKTY DFKDADQYCG
VLVQSPDNLG EVHDWSDLFK HTLKDAKLLK VIGTDLLSLA INKTPKDQGA NVTYGNSQRF
GVPMGFGGPH AAFFAVEDEF KRKMPGRIIG ISKDANGKSA YRMSLQTREQ HIRREKATSN
ICTAQALLAN MAGFYATYHG PQGLQKIANR VNCLARSFAK LAKSLGLVVK EGRIFDTVVL
HNTETLQEYL HYNAQTNVRK IGQDTIFSFD ETHTVQDVED LFNHLQHYTK KKADFMSVIQ
KVIPYKSERA PFLQQKVFNS LHSETEMLRY INYLRQKDVS LTKSMISLGS CTMKLNPTSF
MLPVSFQGFS QLHPFSPLSC TQGYQELTEN VEKWLCDITQ LEAVSLMPNS GAQGEYTGLL
CIRKYHIMNG QKDRNICLIP ISAHGTNPAS AVLAGLTVVP VNVVDGYVDL NDLNKKIKEN
EKSLACIMIT YPSTYGVYED QTKKIIQLIH EHGGLVYMDG ANMNAQVGYT SPGYLGADVC
HLNLHKTFSI PHGGGGPGLG PIAVNKKLAP YLPGREHSLG SVASSLFSSA SILPIPYSYF
GQLGRQGAKK CTAMAMLNAN YLMKSLKDDY KVLFTGQNGM CAHEFIIDIR PIKQESGITE
EDIAKRLMDY GFHAPTMSFP VPGTLMIEPT ESESKSELDR FIEAMKNIKL EIEKVKNGQY
DKNDNPLKNA PHTQDQVINS GWSHKYSREE AAFPLPYVLQ RGKVWPTVSR INNAFGDRNL
ICQCPSVSDY QQ
//
