ID A0KGK7_AERHH Unreviewed; 480 AA.
AC A0KGK7;
DT 12-DEC-2006, integrated into UniProtKB/TrEMBL.
DT 12-DEC-2006, sequence version 1.
DT 27-MAR-2024, entry version 87.
DE RecName: Full=Aspartate ammonia-lyase {ECO:0000256|ARBA:ARBA00016146, ECO:0000256|RuleBase:RU362017};
DE Short=Aspartase {ECO:0000256|RuleBase:RU362017};
DE EC=4.3.1.1 {ECO:0000256|ARBA:ARBA00012992, ECO:0000256|RuleBase:RU362017};
GN Name=aspA {ECO:0000313|EMBL:ABK36406.1};
GN OrderedLocusNames=AHA_0856 {ECO:0000313|EMBL:ABK36406.1};
OS Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC
OS 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Aeromonas.
OX NCBI_TaxID=380703 {ECO:0000313|EMBL:ABK36406.1, ECO:0000313|Proteomes:UP000000756};
RN [1] {ECO:0000313|EMBL:ABK36406.1, ECO:0000313|Proteomes:UP000000756}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 /
RC KCTC 2358 / NCIMB 9240 / NCTC 8049
RC {ECO:0000313|Proteomes:UP000000756};
RX PubMed=16980456; DOI=10.1128/JB.00621-06;
RA Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.,
RA Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S.,
RA Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades.";
RL J. Bacteriol. 188:8272-8282(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-aspartate = fumarate + NH4(+); Xref=Rhea:RHEA:16601,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:29806, ChEBI:CHEBI:29991; EC=4.3.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001494,
CC ECO:0000256|RuleBase:RU362017};
CC -!- SIMILARITY: Belongs to the class-II fumarase/aspartase family.
CC Aspartase subfamily. {ECO:0000256|ARBA:ARBA00005596,
CC ECO:0000256|RuleBase:RU362017}.
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DR EMBL; CP000462; ABK36406.1; -; Genomic_DNA.
DR RefSeq; WP_011704801.1; NC_008570.1.
DR RefSeq; YP_855398.1; NC_008570.1.
DR AlphaFoldDB; A0KGK7; -.
DR STRING; 380703.AHA_0856; -.
DR EnsemblBacteria; ABK36406; ABK36406; AHA_0856.
DR GeneID; 77272838; -.
DR KEGG; aha:AHA_0856; -.
DR PATRIC; fig|380703.7.peg.859; -.
DR eggNOG; COG1027; Bacteria.
DR HOGENOM; CLU_021594_4_1_6; -.
DR OrthoDB; 9802809at2; -.
DR Proteomes; UP000000756; Chromosome.
DR GO; GO:0008797; F:aspartate ammonia-lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0006531; P:aspartate metabolic process; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR CDD; cd01357; Aspartase; 1.
DR Gene3D; 1.10.40.30; Fumarase/aspartase (C-terminal domain); 1.
DR Gene3D; 1.20.200.10; Fumarase/aspartase (Central domain); 1.
DR Gene3D; 1.10.275.10; Fumarase/aspartase (N-terminal domain); 1.
DR InterPro; IPR004708; ApsA.
DR InterPro; IPR024083; Fumarase/histidase_N.
DR InterPro; IPR018951; Fumarase_C_C.
DR InterPro; IPR020557; Fumarate_lyase_CS.
DR InterPro; IPR000362; Fumarate_lyase_fam.
DR InterPro; IPR022761; Fumarate_lyase_N.
DR InterPro; IPR008948; L-Aspartase-like.
DR NCBIfam; TIGR00839; aspA; 1.
DR PANTHER; PTHR42696; ASPARTATE AMMONIA-LYASE; 1.
DR PANTHER; PTHR42696:SF2; ASPARTATE AMMONIA-LYASE; 1.
DR Pfam; PF10415; FumaraseC_C; 1.
DR Pfam; PF00206; Lyase_1; 1.
DR PRINTS; PR00145; ARGSUCLYASE.
DR PRINTS; PR00149; FUMRATELYASE.
DR SUPFAM; SSF48557; L-aspartase-like; 1.
DR PROSITE; PS00163; FUMARATE_LYASES; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|RuleBase:RU362017, ECO:0000313|EMBL:ABK36406.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000756}.
FT DOMAIN 15..347
FT /note="Fumarate lyase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00206"
FT DOMAIN 413..465
FT /note="Fumarase C C-terminal"
FT /evidence="ECO:0000259|Pfam:PF10415"
SQ SEQUENCE 480 AA; 52338 MW; 6B580E9C473BBE2D CRC64;
MSDIKNVRIE EDLLGTKEVS NDLYYGVHTL RAVENFKIST QKISDVPEFV RGMVLTKKAA
ALANGELGTI RPEIADKIVE ACDLMLNTGK CFDQFPVDVY QGGAGTSVNM NTNEVLANIA
LEIMGLAKGR YDVINPNDHV NKCQSTNDAY PTGFRVAVVN STDTTLHALE ALIAAFDAKA
AEFKGILKMG RTQLQDAVPM TLGQEFHAFA VTLREEIKSI KRCQELLLEV NLGATAIGTG
LNTPPEYSAL AIKRLAEITG RAYVPAEDLI EATSDCGAYV MLHGAIKRMA MKLSKICNDL
RLLSSGPRTA LKEINLPEMQ AGSSIMPAKV NPVIPEVVNQ SCFKVFGNDV TITFAAEAGQ
LQLNVMEPVI GQAMFESLSL MEKACISLRE KCVEGITANP EICMNHVLNS IGIVTYLNPF
IGHHEGDIVG KICAQTGKNV REVVLERGLL TAEQLDEILS IENLMNPQYK AKRYTREATV
//
