ID A0KKV9_AERHH Unreviewed; 687 AA.
AC A0KKV9;
DT 12-DEC-2006, integrated into UniProtKB/TrEMBL.
DT 12-DEC-2006, sequence version 1.
DT 27-MAR-2024, entry version 107.
DE SubName: Full=Glycogen debranching enzyme GlgX {ECO:0000313|EMBL:ABK39366.1};
DE EC=3.2.1.- {ECO:0000313|EMBL:ABK39366.1};
GN Name=glgX-1 {ECO:0000313|EMBL:ABK39366.1};
GN OrderedLocusNames=AHA_2395 {ECO:0000313|EMBL:ABK39366.1};
OS Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC
OS 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Aeromonas.
OX NCBI_TaxID=380703 {ECO:0000313|EMBL:ABK39366.1, ECO:0000313|Proteomes:UP000000756};
RN [1] {ECO:0000313|EMBL:ABK39366.1, ECO:0000313|Proteomes:UP000000756}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 /
RC KCTC 2358 / NCIMB 9240 / NCTC 8049
RC {ECO:0000313|Proteomes:UP000000756};
RX PubMed=16980456; DOI=10.1128/JB.00621-06;
RA Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.,
RA Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S.,
RA Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades.";
RL J. Bacteriol. 188:8272-8282(2006).
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000256|ARBA:ARBA00004229}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
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DR EMBL; CP000462; ABK39366.1; -; Genomic_DNA.
DR RefSeq; WP_011706229.1; NC_008570.1.
DR RefSeq; YP_856910.1; NC_008570.1.
DR AlphaFoldDB; A0KKV9; -.
DR STRING; 380703.AHA_2395; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR EnsemblBacteria; ABK39366; ABK39366; AHA_2395.
DR KEGG; aha:AHA_2395; -.
DR PATRIC; fig|380703.7.peg.2390; -.
DR eggNOG; COG1523; Bacteria.
DR HOGENOM; CLU_011725_1_1_6; -.
DR OrthoDB; 3236218at2; -.
DR Proteomes; UP000000756; Chromosome.
DR GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR GO; GO:0019156; F:isoamylase activity; IEA:UniProt.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11326; AmyAc_Glg_debranch; 1.
DR CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR011837; Glycogen_debranch_GlgX.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR048650; ISOA1-3-like_C.
DR NCBIfam; TIGR02100; glgX_debranch; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21156; ISOA1-3_C; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW Glycosidase {ECO:0000313|EMBL:ABK39366.1};
KW Hydrolase {ECO:0000313|EMBL:ABK39366.1};
KW Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Reference proteome {ECO:0000313|Proteomes:UP000000756};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DOMAIN 155..558
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 459..483
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 465..481
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 687 AA; 77836 MW; A4C7A0513AB9BFAB CRC64;
MRISAGHCRQ LGATPEHDGV NFAIWARLAS RVELLLFASA DDTTPEVIPL SPRLNRTAYY
WHIHIEGIEL GQRYAYRIQG PWRPYYGTRF DADKVLLDPY GRSIELGANY DRWAAARPGS
NLAECAKNRV VDTRHYDWEG DKLPAHSLSR SVIYELHLGG FTKSPSSGVD PTLRGTYLGL
IEKIPYLQSL GVTAVELLPV FQFDPQDSPK GLSNYWGYSP MSFFAPHAQY ASGEDPLTEF
RDMVKALHRA NIEVILDVVY NHTAEGGDDG PTFSFRGIDN EAYYILDANQ KDTNYSGCGN
TFNGAHPVVL RMIMDSLHFW RQEMHVDGFR FDLAAILSRD ESGQPQANAP TLRTIDTDPR
IADIKLIAEA WDAGGLYQVG SLAGARWREW NGQFRDDVRR FLRGDDNAVM PFVERLCGSP
DIYHYHHADP EKSINFVTCH DGFTLWDWAS YNGKHNDANG EQNRDGCDHN FSWNHGQEGP
SEDPQINALR LRQAKNMMVA TLLSVGSPML LMGDEVLRTQ HGNNNGYCQD NDTCWMQWQP
DAMGQEMFRF MKELIQYRKH LFQRPEQESM PLSLTEILRH SEICWHGVNA AQPDFSPHSH
AIAMSALSSE TRLALYVLFN AYWEPLTFNL PSPPKGVGGY WRRILDTALP SPEDISTFGM
PLEGLTREYL AQPRSSCLFI CGADDFY
//
