ID A0LIT0_SYNFM Unreviewed; 669 AA.
AC A0LIT0;
DT 12-DEC-2006, integrated into UniProtKB/TrEMBL.
DT 12-DEC-2006, sequence version 1.
DT 27-MAR-2024, entry version 98.
DE RecName: Full=protein-glutamate methylesterase {ECO:0000256|ARBA:ARBA00039140};
DE EC=3.1.1.61 {ECO:0000256|ARBA:ARBA00039140};
GN OrderedLocusNames=Sfum_1645 {ECO:0000313|EMBL:ABK17332.1};
OS Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB).
OC Bacteria; Thermodesulfobacteriota; Syntrophobacteria; Syntrophobacterales;
OC Syntrophobacteraceae; Syntrophobacter.
OX NCBI_TaxID=335543 {ECO:0000313|EMBL:ABK17332.1, ECO:0000313|Proteomes:UP000001784};
RN [1] {ECO:0000313|EMBL:ABK17332.1, ECO:0000313|Proteomes:UP000001784}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 10017 / MPOB {ECO:0000313|Proteomes:UP000001784};
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E.G.,
RA Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Kim E., Boone D.R., Brockman F., Culley D., Ferry J., Gunsalus R.,
RA McInerney M.J., Morrison M., Plugge C., Rohlin L., Scholten J., Sieber J.,
RA Stams A.J.M., Worm P., Henstra A.M., Richardson P.;
RT "Complete sequence of Syntrophobacter fumaroxidans MPOB.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L-
CC glutamyl-[protein] + methanol; Xref=Rhea:RHEA:23236, Rhea:RHEA-
CC COMP:10208, Rhea:RHEA-COMP:10311, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:29973,
CC ChEBI:CHEBI:82795; EC=3.1.1.61;
CC Evidence={ECO:0000256|ARBA:ARBA00000941};
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00050}.
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DR EMBL; CP000478; ABK17332.1; -; Genomic_DNA.
DR RefSeq; WP_011698502.1; NC_008554.1.
DR AlphaFoldDB; A0LIT0; -.
DR STRING; 335543.Sfum_1645; -.
DR KEGG; sfu:Sfum_1645; -.
DR eggNOG; COG2201; Bacteria.
DR HOGENOM; CLU_410445_0_0_7; -.
DR InParanoid; A0LIT0; -.
DR OrthoDB; 9793421at2; -.
DR Proteomes; UP000001784; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
DR GO; GO:0008984; F:protein-glutamate methylesterase activity; IEA:UniProtKB-EC.
DR GO; GO:0006935; P:chemotaxis; IEA:InterPro.
DR CDD; cd17541; REC_CheB-like; 1.
DR Gene3D; 3.40.50.2300; -; 1.
DR Gene3D; 3.40.50.180; Methylesterase CheB, C-terminal domain; 1.
DR InterPro; IPR035909; CheB_C.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR000673; Sig_transdc_resp-reg_Me-estase.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR PANTHER; PTHR42872; PROTEIN-GLUTAMATE METHYLESTERASE/PROTEIN-GLUTAMINE GLUTAMINASE; 1.
DR PANTHER; PTHR42872:SF6; PROTEIN-GLUTAMATE METHYLESTERASE_PROTEIN-GLUTAMINE GLUTAMINASE; 1.
DR Pfam; PF01339; CheB_methylest; 1.
DR Pfam; PF00072; Response_reg; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF52172; CheY-like; 1.
DR SUPFAM; SSF52738; Methylesterase CheB, C-terminal domain; 1.
DR PROSITE; PS50122; CHEB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:ABK17332.1};
KW Phosphoprotein {ECO:0000256|PROSITE-ProRule:PRU00169};
KW Reference proteome {ECO:0000313|Proteomes:UP000001784}.
FT DOMAIN 314..431
FT /note="Response regulatory"
FT /evidence="ECO:0000259|PROSITE:PS50110"
FT DOMAIN 474..660
FT /note="CheB-type methylesterase"
FT /evidence="ECO:0000259|PROSITE:PS50122"
FT MOD_RES 365
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 669 AA; 74294 MW; 2AFE1AD2A8F932CB CRC64;
MNVLLLDPQQ KYAERLRDLS SVVEGANVIS ADEIRICPSD SANIDIIVAG YDRANPRTLD
ALLEWRSHPH TYLVPCWILA DARSFEAVCQ WPRLSVDRFD EQLDIPAFCS WLETVAEWQQ
NRMQLPGTNR FISHSPLELT TSLALRKASG KLSLFDEEGG EGAFLLRDGY LTGSTLKHLS
GTEAFLEFLT WSKGSYLWEP LDTIEFTDQS SPIEFMFHEA LKLFREANLL YHFVPDLNRS
LTKTESESAL DDGAVYYFAE LKVLYNLIDG KATVRQVVEA SPLSQLRTLC CLSKWFSLGD
VAVVPEESPI PRRGLLIVDD SHLMCKALRD VFAKDPRFEV LGFAHDGLQA LELIEQLKPD
VLTLDMQMPR MDGLTALKHI MIRNPRPVVV VSAFTKETSL LTYQSFKYGA VDVLTKPSGG
NPGSREHESR LIRDRVAQAS CVRIEAAQYI RSGGRSKPAE KDGAFSPEAR DRAEHASNGL
AVILCGSGGF PSLLKMLFSI ENTDRLPTLL GGIALSKKAV ESMVPNMEKD SSMRMTELTP
PGGILLPNSS YLFSCEDSFH LVRENDQTMA RCDQDSAAEH RPFDRLLFSL GDGFGEHAMA
VLLSGTGHDG IEGMEHVRRA GGKTYVLSPE ACLEPDLPRR ILQYGFAQEV KSASELASLL
QNVGSENGK
//
